Abstract:

The biomasses and the phenylalanine ammonia lyase (PAL) activities were tested in suspension-cultured cells of Rhodiola fastigiata under different light treatments, and relationship between development of the secondary metabolism of R. fastigiata and the environment factors were investigated. The results showed that: 1) Biomasses of the suspension-cultured cells changed in a typical S curve. The biomasses reached a linear growth phase for 6 to 9 d, and the PAL activity also showed the similar change. The PAL activity increased rapidly at the exponential growth phase of the suspension-cultured cells, and reached the maximum at the time of maximum dry-mass and decreased to initial level when growth of the cells stopped; 2) Biomasses of the suspension-cultured cells of R. fastigiata showed difference under different light treatments, and the activity of PAL also changed at the same timing. Among these light treatments, the continuous red light treatment was of advantage to the increase of the PAL activity. However, the biomass under red light was obviously lower than that under white light.

Key words: Rhodiola fastigiata, cell suspension culture, light quality, biomass, PAL activity