二次回归正交设计在微型月季组织培养中的应用

doi:10.11707/j.1001-7488.20040434

Scientia Silvae Sinicae ›› 2004, Vol. 40 ›› Issue (4): 189-192.doi: 10.11707/j.1001-7488.20040434

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Application of Quadratic Regressive Factorial Experiment to in vitro Culture of Miniature Rose

Han Xiuhui,Yin Weilun,Wang Huafang

College of Biological sciences and Biotechnology, Beijing Forestry University Beijing100083

Received:2002-11-11 Revised:1900-01-01 Online:2004-07-25 Published:2004-07-25

Abstract

Abstract:

Quadratic regressive factorial experiment was used to optimize concentrations of 6-BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L^-1, NAA 0.33 mg·L^-1into MS midum. The 6-BA and NAA concentrations were in a range of 1.68～2.75 mg·L^-1 and 0.07～0.54 mg·L^-1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.

Key words: Quadratic regression factorial experiment, Miniature rose, in vitro culture, 6_BA, NAA

Han Xiuhui;Yin Weilun;Wang Huafang. Application of Quadratic Regressive Factorial Experiment to in vitro Culture of Miniature Rose[J]. Scientia Silvae Sinicae, 2004, 40(4): 189-192.

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Application of Quadratic Regressive Factorial Experiment to in vitro Culture of Miniature Rose

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