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Scientia Silvae Sinicae ›› 2003, Vol. 39 ›› Issue (1): 69-74.doi: 10.11707/j.1001-7488.20030111

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RAPID PROPAGATION FROM SPROUT CULTURE IN VITRO OF EUCALYPTUS GRANDIS

Shi Daxing,Wang Mili,Shi Yisong,Zeng Ping'an,Liu Biying   

  1. College of Forestry and Horticulture, SAU Ya'an625014；College of Resource and Environment, Chongqing University Chongqing400044；The Forestry Seedling Station of Sichuan Province Chengdu610081；Forestry Bureau of Leshan District, Sichuan Province Leshan614000
  • Received:2002-03-07 Revised:1900-01-01 Online:2003-01-25 Published:2003-01-25

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Abstract:

This paper mainly dealt with the study on shoot organogenesis culture in vitro and plantlet regeneration from sprout explants of Eucalyptus grandis. The organogenesis types of regeneration shoots and clump shoots were discussed. The optimal culture conditions were established by orthogonal tests and single factor tests as follows: (1)Initial media: S+0 5 mg·L-1BA+0.05 mg·L-1 NAA+3%sucrose;(2)Clump bud induction media: S+1.0 mg·L-1BA+0.05 mg·L-1 NAA+3% sucrose;(3)Efficiency seedling induction media: S+0.5 mg·L-1BA+0.1 mg·L-1 NAA+2.0 mg·L-1 biotin+3%sucrose;(4)Robust seedling media: MS(1.5 strength of Fe and organic compound)+4%sucrose;(5)Rooting media:1/2MS+1.0 mg·L-1 ABT+2%sucrose.

Key words: Eucalyptus grandis, Sprout, In vitro culture, Rapid propagation