Abstract:

In this study, experimental materials were taken from thirty-year-old trees of Fraxinus mandshurica Rupr. growing on arboretum of Shenyang Agriculture University. In July and August of 2000 respectively, immature pterodia of F. mandshurica were taken, then surface chemical treatment was made to them with 0.1% mercury bichloride, finally, under aseptic condition, immature embryos were taken out from pterodium and cultivated on initial media. After emergence of seedlings, shoot multiplication culture and rooting culture were made. The result of immature embryo culture showed that it was difficult to obtain sprouts from proembryos culture, however, survival rate and plant percent of immature embryo culture both were high, when embryo root and cotyledon had developed, and immature embryos cultivated on agar-solidified WPM media containing no plant growth regulators were highest, up to 100% and 88.5% respectively. And seedling cultivated on WPM media grew prolifically and average height of seedling was higher compared with that on other media. The shoot multiplication rate of embryo culture of F. mandshurica was low, only 2.125 seedlings per month, when explants were cultivated on WPM+ BA 1 mg·L^-1 +NAA 0.1 mg·L^-1. Rooting of F. mandshurica was easy. Rooting percentage of explants cultivated on QL and WPM media was 66% and 60% respectively.

Key words: Fraxinus mandshurica, Immature embryos, In vitro culture