Abstract:

The bud tips of Fraxinus mandshurica were used as explants to screen the optimum hormone combinations for in vitro plant regeneration and shoot preservation. The results showed that the proper hormone combination for shoot regeneration of Fraxinus mandshurica from bud tips was 2-ip 11.46 μmol·L^-1+IAA 0.45 μmol·L^-1 with N₆ as the basic medium, and the induction rate could reach 91.8%. The proper hormone combination for rooting of the regenerated shoots was IBA 0.29 μmol·L^-1+NAA 0.10 μmol·L^-1 with 1/4N₆ as the basic medium, the rooting rate could reach 99.5%. The optimum hormone combination for shoot in vitro preservation was 2-ip 15.80 μmol·L^-1+KT 4.61 μmol·L^-1 with N₆ as the basic medium, its differentiation rate was lower than 0.5% and the in vitro preservation period could reached to 42 months under room temperature.

Key words: Fraxinus mandshurica, bud tips, in vitro culture, in vitro preservation, hormone combination