桑椹发育中花青素、叶绿素含量变化及相关基因的表达分析

doi:10.11707/j.1001-7488.20140908

摘要/Abstract

摘要：

以新选育果叶兼用品系‘嘉陵40号’桑椹为材料,测定不同发育时期桑椹的花青素和叶绿素的含量,并分析花青素合成相关酶基因、Rubisco编码基因和脱镁叶绿酸a加氧酶基因(PaO)的表达差异。花青素含量随着桑椹发育逐渐上升,叶绿素的含量先下降后上升。与花青素合成有关的酶基因随着桑椹发育呈现不同的表达式,Rubisco编码基因RBCL和RBCS表达量逐渐降低,PaO1和PaO2的表达量都呈先下降后逐渐上升,然后再下降的表达模式。使用ABA、乙烯利和1-MCP等试剂处理桑椹,经分析在生化上ABA和乙烯利能促进花青素合成,抑制叶绿素的合成,在基因表达上能促进花青素合成相关基因的表达,而抑制Rubisco编码基因的表达； 1-MCP能抑制花青素的合成,并对花青素合成基因的表达也具抑制作用。

关键词: 桑椹, 花青素, 叶绿素, 乙烯, ABA, 1-MCP

Abstract:

The contents of anthocyanins and chlorophyll of fruits of Morus alba ‘Changjiang 1’ at the different developmental stages were measured, and the expression levels of anthocyanin biosynthesis related genes, the encoding genes of Rubisco and pheophorbide a oxygenase (PaO) were analyzed. The concentration of anthocyanins increased gradually, while the content of chlorophyll first significantly reduced and then increased during the fruit development process. The anthocyanin related genes showed different patterns, the expression levels of RBCL and RBCS were gradually decreasing, and PaO1 and PaO2 showed a reduction in the expression levels at early stage and increase in the following stage, and then the coding mRNA disappeared at stage S5. ABA, ethephon and 1-MCP were used to treat the mulberry fruit. ABA and ethephon induced the biosynthesis of anthocyanins and chlorophyll, improved the expression levels of anthocyanin related genes and downregulated the expression of Rubisco encoded genes. The 1-MCP can inhibited the biosynthesis of anthocyanins by downregulating the expression levels of anthocyanin related genes.

Key words: mulberry fruit, anthocyanin, chlorophyll, ethylene, ABA, 1-MCP

中图分类号:

S718.43

刘长英, 李军, 赵爱春, 王茜龄, 吕蕊花, 王晓红, 鲁成, 余茂德. 桑椹发育中花青素、叶绿素含量变化及相关基因的表达分析[J]. 林业科学, 2014, 50(9): 59-66.

Liu Changying, Li Jun, Zhao Aichun, Wang Xiling, Lü Ruihua, Wang Xiaohong, Lu Cheng, Yu Maode. Changes of Anthocyanin and Chlorophyll Content, and Expression Levels of Related Genes during Development Process of Mulberry Fruit[J]. Scientia Silvae Sinicae, 2014, 50(9): 59-66.

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