关键词: 茶条槭, 悬浮培养, 没食子酸, 细胞生长, 动力学

Abstract:

In this paper we investigated kinetics parameters during the cell culture of Acer ginnala, such as cell growth, consumption of the carbon, nitrogen and phosphorus, and change in the pH and electrical conductivity in the medium, as well as changes in cell fresh weight and dry weight at the various stages in the culture procedure. 1) The cell suspension culture cycle lasted about 15 days by which the maximum biomass in dry weight and the gallic acid content reached to 11.3 g·L^-1 and 0.49%, and the maximum specific rates of cell growth and gallic acid synthesis were 0.541 d^-1 and 0.682 d^-1, respectively. The specific gallic acid production rate was relatively high when the cell growth rate was 0.3-0.4 d^-1. Gallic acid accumulation was partially-growth-associated. 2) The electrical conductivity of the culture medium gradually descended during culture procedure, reached to the lowest point on the 21^st day, and then slightly increased. 3) After 15 days of the cell culture, sucrose and phosphate of the medium almost all were consumed. Ammonium was rapidly adsorbed at the early stage and was consumed on the 12^th day. Compared with ammonium, the absorption of nitrate was slow, it was not absorbed fastly until the 6^th day and the absorption rate reached to the lowest point on the 15^th day.

Key words: Acer ginnala, suspension culture, gallic acid, cell growth, kinetics