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林业科学 ›› 2012, Vol. 48 ›› Issue (10): 18-23.doi: 10.11707/j.1001-7488.20121004

• 论文 • 上一篇    下一篇

茶条槭悬浮培养的动力学

董杰, 詹亚光, 任健   

  1. 东北林业大学生命科学学院 哈尔滨 150040
  • 收稿日期:2011-08-26 修回日期:2012-08-17 出版日期:2012-10-25 发布日期:2012-10-25
  • 通讯作者: 詹亚光

Kinetics in Suspension Culture of Acer ginnala

Dong Jie, Zhan Yaguang, Ren Jian   

  1. College of Life Sciences, Northeast Forestry University Harbin 150040
  • Received:2011-08-26 Revised:2012-08-17 Online:2012-10-25 Published:2012-10-25

摘要:

对茶条槭细胞培养动力学进行研究,在培养周期内不同的培养阶段测定茶条槭细胞生长和培养基中碳源、氮源、磷源的消耗,电导率的变化,以及细胞的鲜质量与干质量的变化,从而了解细胞生长、营养消耗与次生代谢产物积累的基本规律,为建立结构化动力学模型奠定基础。研究结果表明: 1)茶条槭细胞悬浮培养周期约为15天,经过15天的悬浮培养,最大生物量和没食子酸含量分别达到了11.3 g·L-1和0.49%。细胞的最大比生长速率和没食子酸的最大比生成速率分别为0.541 d-1和0.682 d-1。没食子酸的比生成速率当细胞比生长速率在0.3~0.4 d-1时较高。没食子酸的积累和茶条槭细胞的生长模型为部分生长偶联型。2)培养过程中培养基的电导率逐渐下降,到第21天时达到最低点,之后电导率略微上升。3) 经过15天的培养后,培养基中磷源和蔗糖,消耗殆尽; 在细胞培养前期细胞吸收铵盐较快, 12天时已消耗殆尽,而细胞吸收硝酸盐开始速度较慢,6天细胞开始大量吸收硝酸盐,15天时培养基中的硝酸盐达到最低值。

关键词: 茶条槭, 悬浮培养, 没食子酸, 细胞生长, 动力学

Abstract:

In this paper we investigated kinetics parameters during the cell culture of Acer ginnala, such as cell growth, consumption of the carbon, nitrogen and phosphorus, and change in the pH and electrical conductivity in the medium, as well as changes in cell fresh weight and dry weight at the various stages in the culture procedure. 1) The cell suspension culture cycle lasted about 15 days by which the maximum biomass in dry weight and the gallic acid content reached to 11.3 g·L-1 and 0.49%, and the maximum specific rates of cell growth and gallic acid synthesis were 0.541 d-1 and 0.682 d-1, respectively. The specific gallic acid production rate was relatively high when the cell growth rate was 0.3-0.4 d-1. Gallic acid accumulation was partially-growth-associated. 2) The electrical conductivity of the culture medium gradually descended during culture procedure, reached to the lowest point on the 21st day, and then slightly increased. 3) After 15 days of the cell culture, sucrose and phosphate of the medium almost all were consumed. Ammonium was rapidly adsorbed at the early stage and was consumed on the 12th day. Compared with ammonium, the absorption of nitrate was slow, it was not absorbed fastly until the 6th day and the absorption rate reached to the lowest point on the 15th day.

Key words: Acer ginnala, suspension culture, gallic acid, cell growth, kinetics

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