欢迎访问林业科学,今天是

林业科学 ›› 2012, Vol. 48 ›› Issue (10): 24-29.doi: 10.11707/j.1001-7488.20121005

• 论文 • 上一篇    下一篇

沙冬青细胞对MeJA处理的初始生理响应

高海波1,2   

  1. 1. 临沂大学生命科学学院 临沂 276005;2. 北京林业大学生物科学与技术学院 北京 100083
  • 收稿日期:2012-04-25 修回日期:2012-06-06 出版日期:2012-10-25 发布日期:2012-10-25

Primary Physiological Responses of Ammopiptanthus mongolicus Cells Induced by Methyl Jasmonate

Gao Haibo1,2   

  1. 1. College of Life Science, Linyi University Linyi 276005;2. College of Biological Science and Biotechnology, Beijing Forestry University Beijing 100083
  • Received:2012-04-25 Revised:2012-06-06 Online:2012-10-25 Published:2012-10-25

摘要:

研究沙冬青细胞经茉莉酸甲酯(MeJA)瞬时处理后发生的Ca2+离子流、H2O2含量及质膜电位的变化情况。结果表明: MeJA引起沙冬青细胞H+质子内流、H2O2积累及膜电位去极化。胞内钙库抑制剂钌红预处理细胞后,抑制了MeJA处理引起的H+质子内流、H2O2的积累及膜电位去极化; 表明MeJA瞬时处理后,胞内钙库中Ca2+的释放是细胞随后产生的响应 。H2O2清除剂预处理彻底抑制MeJA引起的H+质子内流及质膜电位的去极化反应,证明H2O2的积累位于H+质子内流的上游,H+质子内流是沙冬青细胞膜电位去极化的主要原因。

关键词: 茉莉酸甲酯, 沙冬青, Ca2+离子流, H2O2, 膜电位

Abstract:

This study investigated changes in Ca2+ flux, hydrogen peroxide content and membrane potential of Ammopiptanthus mongolicus cells in response to methyl jasmonate treatment. Results showed that methyl jasmonate caused proton influx, hydrogen peroxide production and membrane depolarization, which were completely inhibited by pretreatment with intracellular calcium store release inhibitor ruthenium red, demonstrating that calcium release from intracellular calcium store was necessary for proton influx, hydrogen peroxide production and membrane depolarization. Both proton influx and membrane depolarization, triggered by treatment with methyl jasmonate, were inhibited by pretreatment with a hydrogen peroxide scavenger, indicating that hydrogen peroxide production was also necessary for proton influx which was the main reason of membrane depolarization.

Key words: methyl jasmonate, Ammopiptanthus mongolicus, Ca2+ ion flux, hydrogen peroxide, membrane potential

中图分类号: