欢迎访问林业科学,今天是

林业科学 ›› 2003, Vol. 39 ›› Issue (6): 33-39.doi: 10.11707/j.1001-7488.20030607

• 论文及研究报告 • 上一篇    下一篇

核桃子叶不定根发生调控的研究

裴东 袁丽钗 谷瑞升 蒋湘宁   

  1. 北京林业大学定向博士生;中国林业科学研究院林业研究所,北京100091,邯郸农业高等专科学校;葡萄牙里斯本大学理学院,里斯本1749-016;北京林业大学,北京100083
  • 收稿日期:2003-06-11 修回日期:1900-01-01 出版日期:2003-11-25 发布日期:2003-11-25

REGULATION OF JUGLANS REGIA COTYLEDON RHIZOGENESIS

Pei Dong,Yuan Lichai,Gu Ruisheng,Jiang Xiangning   

  1. The Research Institute of Forestry, CAF Beijing100091;Faculty of Science, Lisbon University, Portugal Lisbon 1749-016;Beijing Forestry University Beijing100083
  • Received:2003-06-11 Revised:1900-01-01 Online:2003-11-25 Published:2003-11-25

摘要:

以核桃成熟或近成熟种实子叶为试材,在1/4DKW基本培养基上其顶部(近胚端)的生根率达100% ,而中部和基部(远胚端)的生根率为0。在生根过程中顶部内源吲哚乙酸(IAA)表现为初期上升,其后稳定在一定水平,当根原基开始生长时(培养4d)出现一个高峰;而中、基部内源IAA水平在培养初期下降,而后维持在一定的水平;蛋白凝胶电泳分析表明:62.8和38.4kDa是与子叶不定根发生相关的蛋白条带,在不定根发生过程中,该带在子叶顶部表达增强,而中部、基部则消失。当培养基中附加一定水平的吲哚丁酸(IBA) (0.5~10mg·L-1)时,子叶中、基部被诱导产生不定根;附加2mg·L-1 IBA ,其内源IAA的变化与子叶顶部在1/4DKW基本培养基上发根时有相似的趋势;同时该处理启动表达了62.8kDa蛋白条带和抑制表达38.4kDa蛋白条带。反之当培养基中附加6-苄氨基腺嘌呤(BAP)又抑制子叶生根,也抑制了与生根相关的IAA变化和蛋白表达。这一结果和良好的研究模式为从分子水平上深入认识木本植物不定根发生机制奠定了基础。

关键词: 核桃, 离体培养, 子叶, 不定根发生, 激素调控, 蛋白条带

Abstract:

In vitro rhizogenesis of Juglans regia cotyledons from mature or near mature fruit was investigated. The top, middle and basal sections of the cotyledon showed different rooting capacity. On 1/4 DKW basal medium supplemented no plant growth regulators, the rooting ratio of the top section was 100%, while the middle and basal had no rhizogeneesis. The endogenous indoleacetic acid (IAA) level in the rooting top sections presented as increasing at the culture beginning, then maintaining at a level during the differentiation of root primordia, and increasing again to form a peak when the primordia started to grow (4 days after culture). However, the IAA in the middle and basal sections without rhizogenesis showed decreasing dramatically at the culture beginning, then maintaining at a certain level. SDS-PAGE demonstrated that 62.8 and 38.4 kDa were the rooting related protein bands in walnut cotyledon. Former up_expressed and later down-expressed in the rhizogenesis. Application of exogenous indolebutyric acid (IBA)(0.5~10 mg·L-1)in the medium had effects to induce the middle and basal sections to root and to regulate their endogenous IAA changes similar to that happened in the rooting of the top section. Meanwhile it up-regulated the expression of the 62.8 kDa proteins and down-regulated 38.4 kDa protein bands. Exogenous 6-benzylaminopurine (BAP) inhibited the rooting of cotyledon top section and restrained the rooting-related changes on IAA and proteins. The system that IBA induced J.regia cotyledon rooting by regulating rooting-related IAA and proteins could be taken as a good research model for the further investigations on molecular rhizogenesis mechanism of woody plants.

Key words: Juglans regia, In vitro culture, Cotyledon, Rhizogenesis, Hormone regulation, Protein band