火炬松胚性愈伤组织诱导和植株再生的研究*

林业科学 ›› 1998, Vol. 34 ›› Issue (3): 115-119.

火炬松胚性愈伤组织诱导和植株再生的研究^*

唐巍¹,欧阳藩¹,郭仲琛²

1. 中国科学院化工冶金研究所生化工程国家重点实验室北京 100080
2. 中国科学院植物研究所北京 100093

收稿日期:1996-10-28 出版日期:1998-05-25 发布日期:1998-05-25

STUDIES ON EMBRYOGENIC CALLUS INDUCTION AND PLANT REGENERATION IN LOBLOLLY PINE

Wei Tang¹,Fan Ouyang¹,Zhongchen Guo²

1. State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Academia Sinica Beijing 100080
2. Institute of Botany, Academia Sinica Beijing 100093

Received:1996-10-28 Online:1998-05-25 Published:1998-05-25

摘要/Abstract

关键词: 火炬松, 体细胞胚胎发生, 植株再生

Abstract:

White translucent gloosy mucilaginous embryogenic callus was induced from mature zygotic embryos of loblolly pine on callus induction medium containing 2, 4-D and BA. Embryonal suspensor masses were formed from embryonic callus cultured on DCR medium supplemented with 2, 4-D, IBA and BA. Early stage somatic embryos developed into late stage somatic embryos on the high osmatic press medium. Late stage somatic embryos developed into cotyledonary somatic embryos on the medium containing ABA, PEG and activiated charcoal. Cotyledonary somatic embryos can germinate and develop furture intergeneration plant when they were isolated and cultured on a hormone-free DCR medium. The highest frequency of plantlet regeneration from somatic embryos is 18.4%.

Key words: Pinus taeda L., Somatic embryogenesis, Plant regeneration

唐巍,欧阳藩,郭仲琛. 火炬松胚性愈伤组织诱导和植株再生的研究^*[J]. 林业科学, 1998, 34(3): 115-119.

Wei Tang,Fan Ouyang,Zhongchen Guo. STUDIES ON EMBRYOGENIC CALLUS INDUCTION AND PLANT REGENERATION IN LOBLOLLY PINE[J]. Scientia Silvae Sinicae, 1998, 34(3): 115-119.

图/表 5

参考文献 17

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