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林业科学 ›› 2014, Vol. 50 ›› Issue (8): 60-67.doi: 10.11707/j.1001-7488.20140809

• 论文与研究报告 • 上一篇    下一篇

火炬松基因资源的育种值预测与选择

刘天颐1, 杨会肖2, 刘纯鑫1, 王金榜3, 黄少伟1   

  1. 1. 广东省森林植物种质创新与利用重点实验室 华南农业大学林学院 广州 510642;
    2. 广东省林业科学研究院 广州 510520;
    3. 英德市林业科学研究所 英德 513055
  • 收稿日期:2014-02-17 修回日期:2014-04-30 出版日期:2014-08-25 发布日期:2014-07-31
  • 基金资助:

    “十二五”农村领域国家科技计划专题“国外松高世代育种和种苗繁育关键技术研究与示范”(2012BAD01B0203);广东省林业科技创新专项资金项目“松杉良种选育和高效栽培技术研究与示范——松树短周期采脂林良种选育、优质栽培及采收加工技术研究与示范”(2011KJCX013-02)。

Prediction of Breeding Values and Selection to the Gene Resources of Loblolly Pine

Liu Tianyi1, Yang Huixiao2, Liu Chunxin1, Wang Jinbang3, Huang Shaowei1   

  1. 1. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm College of Forestry, South China Agricultural University Guangzhou 510642;
    2. Guangdong Academy of Forestry Guangzhou 510520;
    3. Yingde Institute of Forestry Yingde 513055
  • Received:2014-02-17 Revised:2014-04-30 Online:2014-08-25 Published:2014-07-31
  • Contact: 黄少伟

摘要:

以引自美国的258个不同改良程度的火炬松家系作为试验材料,利用基于混合线性模型的 BLUP 方法对258个家系14年生树高和胸径的育种值进行预测。首先以不同的模型分别预测半同胞家系和全同胞家系的育种值,再通过构建亲缘关系矩阵预测所有个体的育种值,对各类群家系和个体育种值的差异进行分析。14年生时,在参试的各个类群中,入选率同为15%的情况下,各类群根据胸径育种值共选出优良家系39个;全同胞家系类群入选家系的胸径遗传增益最高,高于群体平均值1.40 cm,达8.56%。以配合选择方式选出优良单株77个,入选率为1%。入选的优良家系可从美国重复引种,为火炬松良种生产服务。根据火炬松核心-主群体育种系统的需要,入选的优良单株可用以充实第2代育种群体,其中10个进入核心育种群体,其余67个进入主群体。精确的BLUP预测值和基于个体育种值的选择将提高遗传改良进程中的选择效率和遗传增益,但在利用这些先进的统计分析方法提升林木良种选育效率的同时,必须对育种方案与试验设计进行改进完善。当参试家系数较多时,可采用平衡不完全区组设计,把所有参试家系统一在一个试验中,科学安排田间试验,以获得最大的成效。

关键词: 火炬松, 基因资源, 育种值, 选择

Abstract:

This study tested 258 families of Loblolly pine (Pinus taeda) with different genetically improved levels introduced from the USA. A mixed linear model in terms of restricted maximum likelihood estimate (REML) and best linear unbiased prediction (BLUP) was used to predict breeding values of height and diameter at breast height (DBH) for the tested families and single trees at the age of 14 years. The phylogenetic relationship matrix was used to promote the accurate of the prediction. The control-pollinated families had 1.40 cm of average gain for DBH, being the highest gain in the whole trail. Thirty nine excellent families, accounting for 15% of all the tested families, were selected based on the predicted breeding values. These families could be re-introduced from the USA and used for afforestation. By using combined selection 77 individuals, accounting for 1% of all the tested trees, were selected to enrich the second generation breeding population. Ten individuals among them were selected into the second generation nucleus breeding population, remaining 67 into the second generation main breeding population. The accurate prediction based on BLUP and the selection based on individual tree breeding values in the advanced-generation breeding program would improve the accuracy and the genetic gain of selection. The breeding program and the trail design should be improved to achieve the maximum effect when using the advanced statistics method such as ASReml. The balanced incomplete block design (BIB) could be used to conduct the field experiment design when the families involved in the trail were too many, which would help to evaluate the factors in the trail and award the maximum achievement.

Key words: Pinus taeda, gene resource, breeding value, selection

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