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林业科学 ›› 2026, Vol. 62 ›› Issue (3): 161-170.doi: 10.11707/j.1001-7488.LYKX20250492

• 研究论文 • 上一篇    下一篇

基于表型及功能成分的果用银杏优良栽培品系综合评价

王语1,2,汪贵斌1,2,*(),张源辉1,2,杨玉霞1,2,唐玮1,2,国靖1,2,刘玉华3,余鹏飞4   

  1. 1. 林木遗传育种全国重点实验室 南方现代林业协同创新中心 南京林业大学 南京 210037
    2. 江苏省农业种质资源保护与利用平台 南京 210014
    3. 江苏农林职业技术学院现代林业技术创新研究院 句容 212499
    4. 睢宁县润企投资有限公司 睢宁 221200
  • 收稿日期:2025-08-05 修回日期:2025-10-21 出版日期:2026-03-15 发布日期:2026-03-12
  • 通讯作者: 汪贵斌 E-mail:gbwang@njfu.com.cn
  • 基金资助:
    林木遗传育种国家重点实验室自主研究课题(SKLTGB-NJ2024-005);江苏省高等学校优势学科建设项目(PAPD)。

Comprehensive Evaluation of Superior Ginkgo biloba Cultivars for Fruit Production Based on Phenotype and Functional Components

Yu Wang1,2,Guibin Wang1,2,*(),Yuanhui Zhang1,2,Yuxia Yang1,2,Wei Tang1,2,Jing Guo1,2,Yuhua Liu3,Pengfei Yu4   

  1. 1. State Key Laboratory of Tree Genetics and Breeding Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing 210037
    2. Jiangsu Provincial Platform for Conservation and Utilazation of Agricultural Germplasm Nanjing 210014
    3. School of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry Jurong 212499
    4. Suining Runqi Investment Co., Ltd. Suining 221200
  • Received:2025-08-05 Revised:2025-10-21 Online:2026-03-15 Published:2026-03-12
  • Contact: Guibin Wang E-mail:gbwang@njfu.com.cn

摘要:

目的: 探究银杏种仁主要药用成分(黄酮和内酯)在发育过程中的动态积累规律,分析不同栽培品系间表型性状、种仁营养成分、药用成分和致敏性成分差异,筛选药用成分含量高、综合品质优良的银杏果用栽培品系。方法: 选取4个丰产品系,动态测定种仁发育过程中的药用成分含量变化,并于成熟期系统测定36个栽培品系的种核表型特征、种仁营养成分、药用成分和致敏性成分含量,综合应用变异系数分析、多样性指数分析、相关性分析、主成分分析和模糊隶属函数法,评价各品系优劣,筛选目标品系。结果: 银杏种仁总黄酮和总内酯含量在发育期均呈先升高后降低的变化趋势,峰值分别出现在7月或9月,10月成熟期最低。36个供试品系表型性状变异系数为10.46%~36.88%,多样性指数为3.54~3.58,化学成分变异系数为9.91%~36.93%,多样性指数范围为3.52~3.58。相关性分析显示,淀粉含量与银杏酸含量呈极显著负相关(P < 0.01),与总黄酮含量呈显著负相关(P < 0.05);药用成分(黄酮和内酯)与形态指标(核形系数和核宽、核厚、单核质量)均呈显著负相关(P < 0.05)。基于主成分分析和模糊隶属函数法构建综合评价模型,筛选出25和33号2个药用成分含量显著较高的果用品系,总黄酮含量均值为2.32 mg·g–1,总内酯含量均值为3.66 mg·g–1结论: 本研究阐明银杏种仁药用成分在发育过程中呈先升高后降低的动态积累规律,证实银杏栽培品系在表型性状、营养成分、药用成分等方面表现较高的变异系数,且各性状间存在显著相关性;综合评价出25和33号2个优良栽培品系,尤其是25号药用成分含量突出,且致敏成分相对较低,表现出优异的综合品质,可为“高药用、低致敏”银杏果用良种的定向选育、功能型食品开发及产业化推广提供材料基础。

关键词: 银杏, 优良品系选育, 药用成分, 致敏成分, 综合评价

Abstract:

Objective: This study aims to investigate the dynamic accumulation patterns of the main medicinal components (flavonoids and terpene lactones) in the seed kernel of Ginkgo biloba during development, to analyze the phenotypic traits, seed kernel nutrients, medicinal components, and allergenic components among different cultivars, so as to screen ginkgo fruit cultivars with high medicinal content and superior overall quality. Method: Four productive cultivars were selected to dynamically determine the changes in the content of medicinal components during development of the seed kernel. At maturity, the phenotypic characteristics of the nuts and the nutritional, medicinal, and allergenic components of the kernels in 36 cultivars were systematically determined. The coefficient of variation analysis, diversity index analysis, correlation analysis, principal component analysis, and fuzzy subordinate function method were comprehensively applied to evaluate the advantages and disadvantages of each cultivar and to screen the target cultivars. Result: The total flavonoid and total lactone contents in ginkgo seed kernels exhibited a development pattern of first increasing and then decreasing with the peaks occurring in July or September and the lowest in October during the maturation period. The coefficients of variation of the phenotypic traits across the 36 tested cultivars ranged from 10.46% to 36.88%, with diversity indices ranging from 3.54 to 3.58, while the coefficients of variation of the chemical composition ranged from 9.91% to 36.93%, with diversity indices ranging from 3.52 to 3.58. Correlation analyses revealed that starch content was highly significantly negatively correlated with ginkgolic acid content (P<0.01) and significantly negatively correlated with total flavonoid content (P<0.05). Medicinal constituents (flavonoids and terpene lactones) were significantly negatively correlated with the morphological indexes (nut shape coefficients and nut widths, nut thicknesses, and single nut weights) (P<0.05). Based on principal component analysis and the fuzzy subordinate function method, a comprehensive evaluation model was constructed for screening out two fruit cultivars (No. 25 and No. 33) with significantly higher medicinal content. The two cultivars exhibited an average total flavonoid content of 2.32 mg·g–1, and an average total lactone content of 3.66 mg·g–1. Conclusion: This research has systematically elucidated the dynamic accumulation pattern of medicinal components in ginkgo seed kernels, which exhibit an first increasing and then decreasing during development. It is confirmed that cultivated ginkgo cultivars exhibit high coefficients of variation in phenotypic traits, nutritional components, and medicinal constituents, and there are significant correlations between these traits. Two superior cultivars, No. 25 and No. 33, have been comprehensively evaluated. Especially, cultivar No. 25 has outstanding medicinal component content with relatively low allergenic components, demonstrating excellent comprehensive quality. The findings provide a material foundation for the targeted breeding of “high-medicinal, low-allergenic” ginkgo cultivars, and for the development of functional foods and industrial promotion.

Key words: Ginkgo biloba, breeding of superior cultivars, medicinal components, allergenic components, comprehensive evaluation

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