欢迎访问林业科学,今天是

›› 2013, Vol. 49 ›› Issue (2): 33-38.doi: 10.11707/j.1001-7488.20130206

• 论文 • 上一篇    下一篇

植物磺肽素在杂交鹅掌楸体胚发生中的作用

陈金慧, 张艳娟, 吴亚云, 王鹏凯, 王光萍, 施季森   

  1. 南京林业大学森林资源与环境学院 林木遗传与生物技术省部共建教育部重点实验室 南京 210037
  • 收稿日期:2012-07-25 修回日期:2012-11-15 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 施季森

Effects of Phytosulfokine on the Somatic Embryogenesis of Liriodendron hybrids(L. chinense×L. tulipifera)

Chen Jinhui, Zhang Yanjuan, Wu Yayun, Wang Pengkai, Wang Guangping, Shi Jisen   

  1. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education College of Forest Resources and Environment, Nanjing Forestry University Nanjing 210037
  • Received:2012-07-25 Revised:2012-11-15 Online:2013-02-25 Published:2013-02-25

摘要: 植物磺肽素(PSK),是近年来发现的一种新型植物肽类生长调节物质,具有促进单细胞的分裂增殖等生理功能。以杂交鹅掌楸未成熟胚来源的体胚发生体系为材料,探索PSK-α在植物体胚发生中的作用。研究发现PSK-α能够促进细胞分裂和增殖,有利于胚性细胞的形成。在杂交鹅掌楸体胚发生初期添加0.1 mg·L-1的PSK-α可提高未成熟胚的脱分化率并能有效改善培养细胞的状态; 0.5 mg·L-1的PSK-α能够促进胚性愈伤组织的形成和增殖; 体胚形成后期添加0.8 mg·L-1的PSK-α利于其正常发育以及再生植株的培养。

关键词: 杂交鹅掌楸, 体细胞胚胎发生, 胚性愈伤组织, 再生植株, 植物磺肽素

Abstract: Phytosulfokine (PSK),a new type of plant growth regulator, has multiple biological activities besides promoting single cell proliferation. In this paper, we imvestigated the role of PSK-α during somatic embryogenesis of Liriodendron hybrids(L. chinense×L. tulipifera) using immature zygotic embryos as explants. The result showed that PSK-α was able to improve cell division and proliferation and induce embryogenic cells formation. At the early stage of somatic embryogenesis, 0.1 mg·L-1 PSK-α increased the ratio of callus formation from explants and improved cell state; 0.5 mg·L-1 PSK-α induced embryogenic callus formation; and 0.8 mg·L-1 PSK-α was in favor of somatic embryo development and growth of regeneration plantlets.

Key words: Liriodendron hybrids, somatic embryogenesis, embryogenic callus, plantlet regeneration, PSK

中图分类号: