关键词: 丽江云杉, 体细胞胚胎发生, 胚性愈伤组织

Abstract:

Mature zygotic embryos of Picea likiangensis were used as explants to investigate effects of explant genotype, basic media and plant growth regulators on the somatic embryogenesis. The optimal media for the somatic embryogenesis at four stages were screened out: 1) P₆+0.5 mg·L^-1 6-BA+1.5 mg·L^-1 2,4-D was optimal medium for initiating somatic callus; 2) P₆+0.5 mg·L^-1 6-BA+1.0 mg·L^-1 2,4-D was optimal medium for maintaining and proliferation; 3) P₆+20 mg·L^-1 ABA was optimal medium for mature somatic embryos; 4) P₆+5 g·L^-1 activated charcoal+5 g·L^-1 sugar was optimal medium for germination.

Key words: Picea likiangensis, somatic embryogenesis, embryogenic callus