关键词: 鹅掌楸, 北美鹅掌楸, 杂交鹅掌楸, SSR分子标记, 特异性鉴定

Abstract:

This paper reported that speciesspecific SSR markers were used to identify Liriodendron tulipifera, L. chinense and hybrid Liriodendron. Overall, 90 genotypes were taken as DNA amplification templates, of which 30 came from 20 provenances of L. chinense, 30 from 5 provenances of L. tulipifera, and 30 from 30 mating combinations of hybrid Liriodendron. Upon two round amplifying and screening, one speciesspecific pair primers for Liriodendron were screened from 176 pair primers. With the pair primers, a 190 bp fragment from L. chinense, 180 bp fragment from L. tulipifera, and a 190 bp and a 180 bp fragment from hybrid Liriodendron were respectively amplified. To testify the veracity of these pair primers, 10 individuals from L. chinense, 5 from L. tulipifera, and 10 from hybrid Liriodendron were used as amplification templates, and the results showed 100% veracity for species identification. Therefore, the development of the pair primers provided an effective, stable and reliable method for speciesspecific identification in Liriodendron at the molecular level.

Key words: Liriodendron tulipifera, Liriodendron chinense, hybrid Liriodendron, SSR marker, specific identification