植物促生微生物对枫香叶色素组成和根际微生物群落的影响

doi:10.11707/j.1001-7488.LYKX20230447

摘要/Abstract

摘要：

目的: 在枫香根际接种植物促生微生物（PGPM），研究PGPM对土壤理化性质和枫香叶色的影响以及对枫香根际微生物群落组成结构和多样性特征的改变，探明外源PGPM对植物和土壤微生物的影响。方法: 以句容市句容林场长势一致的7年生枫香为对象，通过田间接种试验并利用Illumina MiSeq高通量测序技术研究接种黏质沙雷氏菌（NJ2D）、摩西斗管囊霉（BJ04）对枫香根际土壤理化性质、叶色参数及根际土壤微生物群落结构和多样性特征的影响。结果: 1）处理组的枫香根际土壤全氮、全磷、土壤有机质、有效磷含量、土壤含水率（%）和土壤pH较对照均显著提高（P < 0.05）；2）单接种BJ04菌剂的枫香叶片中叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量均较对照显著提高（P < 0.05），而双接种NJ2D+BJ04菌剂枫香叶片的花青素含量显著提高（P < 0.05）；3）对照与处理组的枫香根际微生物多样性也存在显著差异（P < 0.05），其中，单接种NJ2D、BJ04和双接种NJ2D+BJ04菌剂的枫香根际细菌的OTU（操作分类单元）数量较对照均显著增加（P < 0.05），而丛枝菌根真菌（AMF）的OTU数量较对照均显著降低（P < 0.05）；4）根际微生物高通量测序结果显示，放线菌门、变形菌门、绿弯菌门、酸杆菌门和芽单胞菌门为枫香根际土壤中主要优势细菌菌群；球囊霉属、根生囊霉属和Dominikia为枫香根际土壤中主要AMF的优势菌群。接种处理后，枫香根际的酸杆菌门、绿弯菌门和浮霉菌门的物种丰度较对照均显著减小（P < 0.05），而变形菌门和放线菌门的物种丰度较对照则显著增加（P < 0.05）。结论: 接种解磷菌、丛枝菌根真菌以及复合菌剂NJ2D+BJ04均可改善土壤微环境，并对枫香秋季叶色变化产生影响。

关键词: 根际微生物群, 土壤理化性质, 叶色素, 微生物互作, 枫香

Abstract:

Objective: In this study, plant growth-promoting microorganisms (PGPM) were used to inoculate into the rhizosphere of Liquidambar formosana to investigate effects of PGPM on soil physicochemical properties and the leaf color of L. formosana, as well as changes in the composition and diversity characteristics of the rhizosphere microbial community, in order to study the effect of PGPM on plants and soil microorganisms. Method: A field inoculation experiment was conducted on 7-year-old L. formosana seedlings with consistent growth in Jurong Forest Farm of Jurong City with phosphate solubilizing bacteria Serratia marcescens subsp. marcescens (NJ2D) and Funneliformis mosseae (BJ04), and the effects on soil physical and chemical properties, leaf color parameters in autumn and soil microbial community were analyzed. Result: 1) The total nitrogen, total phosphorus, soil organic matter, available phosphorus content, soil moisture content (%), and soil pH of the rhizosphere soil of L. formosana in the treatment group were significantly increased compared to the control group (P<0.05). 2) The content of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in leaves of L. formosana inoculated once with BJ04 significantly increased (P<0.05), while NJ2D+BJ04 inoculation significantly increased the anthocyanin level in the leaves (P<0.05). 3) There were significant differences in the rhizosphere soil microbial community diversity between control group (CK), NJ2D, BJ04 and NJ2D+BJ04 treatments (P<0.05). The number of OTUs (operating taxonomic units) of rhizosphere bacteria in L. formosana trees inoculated with NJ2D, BJ04, and NJ2D+BJ04 was significantly increased compared to the control group (P<0.05), while the number of OTUs of arbuscular mycorrhizal fungi (AMF) was significantly reduced compared to the control group (P<0.05). 4) The high-throughput sequencing results of rhizosphere microorganisms showed that Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria and Gemmatimonadetes dominated the bacterial communities on the rhizosphere of L. formosana. Within the AMF community, Glomus, Rhizophagous and Dominika were dominant genera. However, after inoculation, the species abundances of Acidobacteria, Chloroflexi and Planctomycetes were significantly reduced within the rhizosphere of L. formosana (P<0.05), whereas Proteobacteria and Actinobacteria were significantly increased (P<0.05). Conclusion: Inoculation with phosphorus solubilizing bacteria, arbuscular mycorrhizal fungi, and composite microbial agents NJ2D+BJ04 can all influence the soil microenvironment and have an impact on the autumn colour of L. formosana leaves.

Key words: rhizosphere microbiome, soil physicochemical properties, leaf pigment, biological interaction, Liquidambar formosana

中图分类号:

S725
Q938

吴祝华,宋娟,朱树林,赵邢,杨学祥,任嘉红,陈凤毛. 植物促生微生物对枫香叶色素组成和根际微生物群落的影响[J]. 林业科学, 2023, 59(12): 125-136.

Zhuhua Wu,Juan Song,Shulin Zhu,Xing Zhao,Xuexiang Yang,Jiahong Ren,Fengmao Chen. Effects of Plant Growth-Promoting Microorganisms on Rhizosphere Microbial Community and the Leaf Pigment Composition of Liquidambar formosana[J]. Scientia Silvae Sinicae, 2023, 59(12): 125-136.

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处理Treatment	全氮含量 Total nitrogen content /(g·kg^?1)	全磷含量 Total phosphorus content /(g·kg^?1)	全钾含量 Total potassium content /(g·kg^?1)	有机质含量 Organic matter content /(g·kg^?1)	有效磷含量 Available phosphorus content /(mg·kg^?1)	含水率 Water content （%）	pH
CK	0.82±0.03b	0.114±0.00 b	14.25±0.09a	7.14 ±0.18c	0.22±0.02d	2.5±0.2b	6.88±0.12d
NJ2D	0.94±0.05ab	0.116 ±0.002ab	13.99±0.65a	9.78 ±0.26b	0.78 ±0.02b	2.8±0.1ab	8.40±0.08a
BJ04	0.88±0.06b	0.120 ±0.001a	14.93±0.42a	13.21±0.99a	1.05 ±0.03a	3.0±0.1a	7.22 ±0.03c
NJ2D+BJ04	1.09±0.07a	0.119 ±0.001ab	13.96±0.62a	13.88 ±0.67a	0.31 ±0.03c	3.1±0.5a	7.88±0.05b

项目Item	多样性指数Diversity	CK	NJ2D	BJ04	NJ2D+BJ04
细菌 Bacteria	chao1	3 345.01 ±105.89b	4 335.96 ±158.30a	4 239.23 ±78.57 a	4 379.43 ±266.44 a
	Shannon	9.24 ±0.05b	9.69 ±0.09a	9.54 ±0.10 a	9.68 ±0.11 a
	goods_coverage	0.987 ±0.000a	0.981 ±0.001b	0.982 ±0.001 b	0.986 ±0.001a
AMF	chao1	286.93 ±1.08a	227.25 ±10.42c	251.07 ±5.06 b	232.32 ±8.20 bc
	Shannon	3.62 ±0.03b	3.40 ±0.06c	4.03 ±0.04 a	3.89 ±0.02 a
	goods_coverage	0.999 ±0.000a	0.999 ±0.000a	0.999 ±0.000 a	0.999 ±0.000 a

指标Item	pH	含水率 Water content	有效磷含量 Available phosphorus	有机质含量 Organic matter	全钾含量 Total potassium
土壤全磷含量 Total phosphorus content	?0.323	?0.072	0.214	0.495	0.235
土壤全氮含量 Total nitiogen content	?0.037	0.296	?0.351	0.632**	0.497
类胡萝卜素含量 Carotenoids content	?0.683**	0.174	0.662**	0.160	0.168
花青素含量 Anthocyanin content	0.087	0.128	?0.491	0.477	?0.203
叶绿素a含量 Chlorophyll a content	?0.654*	0.207	0.649**	0.242	0.136
叶绿素b含量 Chlorophyll b content	?0.637**	0.211	0.654*	0.252	0.128
总叶绿素含量 Total chlorophyll content	?0.650**	0.208	0.650*	0.245	0.134
chao1(Bacteria)	0.538	0.014	0.073	0.042	0.030
chao1(AMF)	?0.789**	?0.125	0.175	?0.298	0.011

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