林业科学 ›› 2023, Vol. 59 ›› Issue (6): 141-148.doi: 10.11707/j.1001-7488.LYKX20210766
邹春阳,吴文娟*
收稿日期:
2021-10-13
出版日期:
2023-06-25
发布日期:
2023-08-08
通讯作者:
吴文娟
基金资助:
Chunyang Zou,Wenjuan Wu*
Received:
2021-10-13
Online:
2023-06-25
Published:
2023-08-08
Contact:
Wenjuan Wu
摘要:
目的: 以稻草、芦草和竹子中不同结构单元木质素为研究对象,借助耗散型石英晶体微天平(QCM-D)技术,原位、实时探究不同结构单元分离木质素与酶吸附的动态行为,为木质素对生物质酶水解的影响提供理论基础、对木质纤维素的高效利用进行定向调控。方法: 原料化学成分参照标准方法测定,分离木质素结构经红外、凝胶渗透色谱和碱性硝基苯氧化表征,采用原子力显微镜观察木质素薄膜表面形貌,借助QCM-D技术分析纤维素酶在木质素薄膜上的吸附行为。结果: 分离木质素均为GSH型木质素,纯度高,含糖量约10%,分子质量接近,约5 000;硝基苯氧化结果显示,稻草、芦草和竹子中木质素结构单元存在差异,竹子中木质素未缩合单元得率最高,为444.4 g·kg?1,S/G比为1∶0.6,芦草、稻草中木质素S/G比均为1∶1.1;经QCM-D分析,竹子木质素薄膜对纤维素酶吸附速率最快、吸附量最大,芦草和稻草木质素薄膜对纤维素酶的吸附速率和最大吸附量相近;停止通酶后,用缓冲液冲洗,纤维素酶附着在木质素上难以被洗脱。酶吸附过程中3种木质素表面均具有较好的黏弹性,且黏弹性差异不大。结论: 稻草、芦草和竹子中木质素对纤维素酶均有明显吸附作用,来源不同的木质素但结构单元相同,对纤维素酶吸附能力相同;木质素结构单元不同,对纤维素酶吸附能力不同。纤维素酶在木质素薄膜表面上结合紧密,难以脱附,S/G比较高的木质素,其纤维素酶吸附能力较强,在纤维素酶水解中产生更多无效吸附,导致葡聚糖转化效率降低。
中图分类号:
邹春阳,吴文娟. 木质素结构单元对纤维素酶吸附的影响[J]. 林业科学, 2023, 59(6): 141-148.
Chunyang Zou,Wenjuan Wu. Effect of Lignin Structural Unit on Cellulase Adsorption[J]. Scientia Silvae Sinicae, 2023, 59(6): 141-148.
表3
木质素硝基苯氧化得率及结构单元比例①"
木质素 Lignin | 结构单元比例Molar ratio of structural units | 结构单元得率 Yield of structural unit / (g·kg?1) | 总得率 Total yield/ (g·kg?1) | |||||
S | G | H | S | G | H | |||
芦草 Phragmites australis | 1 | 1.1 | 0.7 | 180.9±1.1 | 158.2±1.2 | 81.1±1.1 | 420.2±1.1 | |
稻草 Oryza sativa | 1 | 1.1 | 0.6 | 138.8±1.2 | 124.3±1.2 | 53.1±1.3 | 316.2±1.1 | |
竹子 Bambusoideae | 1 | 0.6 | 0.4 | 248.2±1.2 | 129.9±1.3 | 67.3±1.3 | 444.4±1.2 |
吴文娟, 闫雪晴, 邹春阳, 等. 基于全溶体系的毛竹竹材木质素分离方法. 浙江农林大学学报, 2020, 37 (2): 335- 342.
doi: 10.11833/j.issn.2095-0756.2020.02.019 |
|
Wu W J, Yan X Q, Zou C Y, et al. A isolation method of lignin from bamboo based on complete dissolution. Journal of Zhejiang A & F University, 2020, 37 (2): 335- 342.
doi: 10.11833/j.issn.2095-0756.2020.02.019 |
|
黄丽菁, 吴彩文, 邹春阳, 等. 木质素与酶的作用机制及其在纤维素酶水解中的影响研究进展. 西北林学院学报, 2021, 36 (2): 142- 148.
doi: 10.3969/j.issn.1001-7461.2021.02.21 |
|
Huang L J, Wu C W, Zou C Y, et al. The action mechanism of lignin-enzyme and research progress of its influence on enzymatic hydrolysis. Journal of Northwest Forestry University, 2021, 36 (2): 142- 148.
doi: 10.3969/j.issn.1001-7461.2021.02.21 |
|
Achinas S, Euverink G J W. Consolidated briefing of biochemical ethanol production from lignocellulosic biomass. Electronic Journal of Biotechnology, 2016, 23, 44- 53.
doi: 10.1016/j.ejbt.2016.07.006 |
|
Berg I H, Lindh L, Arnebrant T J B. Intraoral lubrication of PRP-1, statherin and mucin as studied by AFM. Biofouling, 2004, 20 (1): 65- 70.
doi: 10.1080/08927010310001639082 |
|
Bin Y, Hongzhang C. Effect of the ash on enzymatic hydrolysis of steam-exploded rice straw. Bioresource Technology, 2010, 101 (23): 9114- 9119.
doi: 10.1016/j.biortech.2010.07.033 |
|
Bonawitz N D, Kim J I, Tobimatsu Y, et al. Disruption of mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant . Nature, 2014, 509 (7500): 376- 380.
doi: 10.1038/nature13084 |
|
Dos Santos A C, Ximenes E, Kim Y, et al. Lignin-enzyme interactions in the hydrolysis of lignocellulosic biomass. Trends in Biotechnology, 2019, 37 (5): 518- 531.
doi: 10.1016/j.tibtech.2018.10.010 |
|
Dzhumanova Z K, Dalimova G N. Nitrobenzene oxidation of lignins from several plants of the family Gramineae. Chemistry of Natural Compounds, 2011, 47 (3): 419- 421.
doi: 10.1007/s10600-011-9948-7 |
|
Feiler A A, Sahlholm A, Sandberg T, et al. Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D). Journal of Colloid and Interface Science, 315(2): 475-481. | |
Guo F F, Shi W J, Sun W, et al. Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism. Biotechnology for Biofuels, 2014, 7 (1): 1- 10.
doi: 10.1186/1754-6834-7-1 |
|
Jiang C, Cao T Y, Wu W J, et al. Novel approach to prepare ultrathin lignocellulosic film for monitoring enzymatic hydrolysis process by quartz crystal microbalance. ACS Sustainable Chemistry & Engineering, 2017, 5 (5): 3837- 3844. | |
Jin Y C, Jameel H, Chang H M, et al. Green liquor pretreatment of mixed hardwood for ethanol production in a repurposed kraft pulp mill. Journal of Wood Chemistry and Technology, 2010, 30 (1): 86- 104.
doi: 10.1080/02773810903578360 |
|
Ko J K, Ximenes E, Kim Y, et al. Adsorption of enzyme onto lignins of liquid hot water pretreated hardwoods. Biotechnology & Bioengineering, 2015, 112 (3): 447- 456. | |
Li M, Pu Y Q, Ragauskas A J. Current understanding of the correlation of lignin structure with biomass recalcitrance. Frontiers in Chemistry, 2016, 4, 45. | |
Li X, Li M, Pu Y, et al. Inhibitory effects of lignin on enzymatic hydrolysis: the role of lignin chemistry and molecular weight. Renewable Energy, 2018, 123, 664- 674.
doi: 10.1016/j.renene.2018.02.079 |
|
Li X, Zheng Y. Lignin-enzyme interaction: mechanism, mitigation approach, modeling, and research prospects. Biotechnology Advances, 2017, 35 (4): 466- 489.
doi: 10.1016/j.biotechadv.2017.03.010 |
|
Liao J J, Latif N H A, Trache D, et al. Current advancement on the isolation, characterization and application of lignin. International Journal of Biological Macromolecules, 2020, 162, 985- 1024.
doi: 10.1016/j.ijbiomac.2020.06.168 |
|
Nakagame S, Chandra R P, Saddler J N. The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis. Biotechnology and Bioengineering, 2010, 105 (5): 871- 879. | |
Rastogi M, Shrivastava S. Recent advances in second generation bioethanol production: an insight to pretreatment, saccharification and fermentation processes. Renewable and Sustainable Energy Reviews, 2017, 80, 330- 340.
doi: 10.1016/j.rser.2017.05.225 |
|
Sanchez O J, Cardona C A. Trends in biotechnological production of fuel ethanol from different feedstocks. Bioresource Technology, 2008, 99 (13): 5270- 5295.
doi: 10.1016/j.biortech.2007.11.013 |
|
Shi Z J, Xu G F, Deng J, et al. Structural characterization of lignin from D.sinicusby FTIR and NMR techniques . Green Chemistry Letters and Reviews, 2019, 12 (3): 235- 243..
doi: 10.1080/17518253.2019.1627428 |
|
Shuba Eyasu S, Kifle D. Microalgae to biofuels: ‘Promising’ alternative and renewable energy, review. Renewable and Sustainable Energy Reviews, 2018, 81, 743- 755.
doi: 10.1016/j.rser.2017.08.042 |
|
Siqueira G, Arantes V, Saddler J N, et al. Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin. Biotechnology for Biofuels, 2017, 10 (1): 1- 12.
doi: 10.1186/s13068-016-0693-9 |
|
Sluiter A, Hames B, Ruiz R, et al. 2008. Determination of structural carbohydrates and lignin in biomass. National Renewable Energy Laboratory: NREL Report No. TP−510−42618. | |
Studer M H, DeMartini J D, Davis M F, et al. Lignin content in natural populus variants affects sugar release. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (15): 6300- 6305.
doi: 10.1073/pnas.1009252108 |
|
Tan L P, Sun W, Li X Z, et al. Bisulfite pretreatment changes the structure and properties of oil palm empty fruit bunch to improve enzymatic hydrolysis and bioethanol production. Biotechnology Journal, 2015, 10 (6): 915- 925.
doi: 10.1002/biot.201400733 |
|
Tribot A, Amer G, Abdou Alio M, et al. Wood-lignin: supply, extraction processes and use as bio-based material. European Polymer Journal, 2019, 112, 228- 240.
doi: 10.1016/j.eurpolymj.2019.01.007 |
|
Turon X, Rojas O J, Deinhammer R S, et al. Enzymatic kinetics of cellulose hydrolysis: a QCM-D study. Langmuir the ACS Journal of Surfaces & Colloids, 2008, 24 (8): 3880- 3887. | |
Wang W, Zhu Y, Du J, et al. Influence of lignin addition on the enzymatic digestibility of pretreated lignocellulosic biomasses. Bioresour Technol, 2015, 181, 7- 12.
doi: 10.1016/j.biortech.2015.01.026 |
|
Wang Z G, Yokoyama T, Chang H M, et al. Dissolution of beech and spruce milled woods in LiCl/DMSO. Journal of Agricultural and Food Chemistry, 2009, 57 (14): 6167- 6170.
doi: 10.1021/jf900441q |
|
Wu S F, Chang H M, Jameel H, et al. Novel green liquor pretreatment of loblolly pine chips to facilitate enzymatic hydrolysis into fermentable sugars for ethanol production. Journal of Wood Chemistry and Technology, 2010, 30 (3): 205- 218.
doi: 10.1080/02773811003746717 |
|
Wu W J, Wang Z G, Jin Y C, et al. Isolation of cellulolytic enzyme lignin from rice straw enhanced by LiCl/DMSO dissolution and regeneration. BioRresources, 2014, 9 (3): 4382- 4391. | |
Xu C, Liu F, Alam M A, et al. Comparative study on the properties of lignin isolated from different pretreated sugarcane bagasse and its inhibitory effects on enzymatic hydrolysis. International Journal of Biological Macromolecules, 2020, 146, 132- 140.
doi: 10.1016/j.ijbiomac.2019.12.270 |
|
Xu G F, Shi Z, Zhao Y, et al. Structural characterization of lignin and its carbohydrate complexes isolated from bamboo (Dendrocalamus sinicus) . International Journal of Biological Macromolecules, 2019, 126, 376- 384.
doi: 10.1016/j.ijbiomac.2018.12.234 |
|
Yasuda S, Fukushima K, Kakehi A. Formation and chemical structures of acid-soluble lignin I: sulfuric acid treatment time and acid-soluble lignin content of hardwood. Journal of Wood Science, 2001, 47 (1): 69- 72.
doi: 10.1007/BF00776648 |
|
Yoo C G, Meng X, Pu Y, et al. The critical role of lignin in lignocellulosic biomass conversion and recent pretreatment strategies: a comprehensive review. Bioresource Technology, 2020, 301, 122784.
doi: 10.1016/j.biortech.2020.122784 |
|
Zanchetta A, dos Santos A C F, Ximenes E, et al. Temperature dependent cellulase adsorption on lignin from sugarcane bagasse. Bioresource Technology, 2018, 252, 143- 149.
doi: 10.1016/j.biortech.2017.12.061 |
|
Zheng W Q, Lan T Q, Li H, et al. Exploring why sodium lignosulfonate influenced enzymatic hydrolysis efficiency of cellulose from the perspective of substrate-enzyme adsorption. Biotechnology for Biofuels, 2020, 13 (1): 1- 12.
doi: 10.1186/s13068-019-1642-1 |
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