欢迎访问林业科学,今天是

林业科学 ›› 2020, Vol. 56 ›› Issue (7): 194-203.doi: 10.11707/j.1001-7488.20200720

• 研究简报 • 上一篇    下一篇

6个竹种叶色与光合色素含量及叶片结构比较

苏佳露1,2,3,史无双1,2,3,杨雅运1,2,3,王星1,2,3,丁雨龙1,2,林树燕1,2,*   

  1. 1. 南京林业大学 南方现代林业协同创新中心 南京 210037
    2. 南京林业大学竹类研究所 南京 210037
    3. 南京林业大学生物与环境学院 南京 210037
  • 收稿日期:2019-06-09 出版日期:2020-07-25 发布日期:2020-08-11
  • 通讯作者: 林树燕
  • 基金资助:
    江苏省林业科技创新与推广项目(LYKJ[2019]29);‘十三五’重点研发计划课题(2016YFD0600901);江苏高校优势学科建设工程项目(PAPD)

Comparison of Leaf Color and Pigment Content and Observation of Leaf Structure at Different Growth Stages from Six Bamboo Species

Jialu Su1,2,3,Wushuang Shi1,2,3,Yayun Yang1,2,3,Xing Wang1,2,3,Yulong Ding1,2,Shuyan Lin1,2,*   

  1. 1. Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry University Nanjing 210037
    2. Bamboo Research Institute, Nanjing Forestry University Nanjing 210037
    3. College of Biology and Environment, Nanjing Forestry University Nanjing 210037
  • Received:2019-06-09 Online:2020-07-25 Published:2020-08-11
  • Contact: Shuyan Lin

摘要:

目的: 探究6个彩叶竹种在不同分区的叶色与光合色素,以及与细胞显微超微结构之间的关系,为明确6个彩叶竹种的叶色变异机制提供细胞学依据,为揭示竹种叶色变异机制提供参考。方法: 以七彩红竹、菲白竹、靓竹、黄条金刚竹、锦竹、花叶唐竹6个彩叶竹种叶片为材料,将叶片按叶色分为白区和绿区,对叶片不同分区的叶色、叶绿素a、叶绿素b、类胡萝卜素含量进行测定,比较不同生长阶段的叶片显微结构和成熟叶片不同分区超微结构进行观察比较。结果: 6个竹种不同叶色分区中光合色素含量、显微结构、超微结构之间存在明显差异。1)绿区各光合色素含量显著高于白区,其中绿区叶绿素a含量最高,白区类胡萝卜素含量最高。2)黄条金刚竹在叶片成熟后出现白区,七彩红竹、菲白竹、靓竹、锦竹、花叶唐竹在叶片发育中期出现白区,且七彩红竹与黄条金刚竹成熟叶片白区细胞内溶物缺失严重,其余4个竹种白区细胞内容物部分缺失。3)绿区指状臂细胞与梅花状细胞内部结构完整,叶绿体发育正常;白区内部细胞器较少,叶绿体结构异常,不同竹种质体形态不同。结论: 6个彩叶竹种叶片白(黄)色条纹的出现与细胞的排列方式无关;黄条金刚竹黄色条纹的出现,与叶片成熟后一部分叶肉细胞内部光合色素降解和叶绿体结构破坏有关,七彩红竹、菲白竹、靓竹、锦竹和花叶唐竹叶片不同叶色的呈现与一部分细胞内叶绿体发育异常和光合色素含量较低有关;白区类胡萝卜素的含量占比影响叶片黄色深浅度。

关键词: 竹叶, 叶色变异, 光合色素, 叶绿体, 显微结构, 超微结构

Abstract:

Objective: To explore the mechanism of leaf color variation of six bamboo species, we investigated the relationship among leaf color, chlorophyll and carotenoids, and cell ultrastructure in different color stripe of six colorful bamboo species. Method: We collected leaves from six bamboo species. In this study, 6 colorful bamboo species were targeted, and they are Indosasa hispida 'Rainbow' (IH), Pleioblastus fortunei (PF), Sasaella glabra f. albostriata (SG), S. kogasenis 'Aureostriatus' (SK), Hibanobambusa tranquillans f. shiroshima (HT) and Sinobambusa tootsik var. luteoloalbostriata (ST). The leaves were separated into albino stripe and green stripe regions according to leaf color. The pixel value of leaf color and the contents of chlorophyll a, chlorophyll b and carotenoids in different zones of leaves were tested. Meanwhile, the microstructure of leaves in different development stages and the ultrastructure of different color zones of mature leaves were observed and analyzed. Result: There were significant differences in photosynthetic pigment content, microstructure and ultrastructure among different leaf color zones of six bamboo species. The photosynthetic pigment content in green stripe was significantly higher than that in albino stripe. The content of chlorophyll a was the highest in green stripe, and that of carotenoid was the highest in albino stripe. Microstructure of leaves at different developmental stages showed that albino stripe appeared in mature leaves for SK, while albino stripe appeared in the leaves at middle developmental stage for IH, PF, SG, HT, ST. The albino stripe of mature leaves in IH and SK showed the serious deficiency of cytoplasmic substances, while albino stripe in the other four bamboo species showed partial deficiency. The ultrastructure of the leaves of six bamboo species showed that chloroplast distributed in two types of mesophyll cells which are arm cells and rosette cell cells in green stripe. While there were a few organelles and abnormal chloroplasts in albino stripe. And the plastid morphology of six bamboo species were different. Conclusion: The appearance of white (yellow) stripes in leaves of six colorful bamboo species is not related the arrangement of cells. The appearance of yellow stripes in SK is caused by the degradation of photosynthetic pigment and the destruction of chloroplast structure after leaves matured. While the different leaves color in IH, PF, SG, HT and ST are related to the chloroplast dysplasia and low content of photosynthetic pigment in some cells. The proportion of carotenoids in albino stripe affects the yellow degree of leaves.

Key words: bamboo leaves, leaf color variation, photosynthetic pigment, chloroplast, microstructure, ultrastructure

中图分类号: