苦竹叶化学成分对细菌及3种肿瘤细胞的抑制活性

doi:10.11707/j.1001-7488.20150510

摘要/Abstract

摘要：

【目的】评价苦竹叶提取物及其化学成分对细菌和3种肿瘤细胞的离体抑制生长活性,为竹叶化学成分的开发利用奠定基础。【方法】采用95%乙醇提取和柱色谱分离,从苦竹叶中获得提取物流分及单体化合物; 以大肠杆菌、金黄色葡萄球菌及枯草芽孢杆菌为研究对象,采用抑菌圈法评价苦竹叶提取物各流分及单体化合物的离体抑菌活性; 采用MTT法和小鼠H22肝癌细胞腹水瘤模型,评价木犀草素-6-C-洋地黄毒糖苷-4'-O-葡萄糖苷的体内外抗肿瘤活性。【结果】苦竹叶乙醇提取物萃取流分中,乙酸乙酯相的离体抑菌作用整体强于石油醚相和正丁醇相,水相抑菌作用不明显。乙酸乙酯相8个流分中Fr.3的离体抑菌作用最强,在25 mg·mL^-1浓度下,对枯草芽孢杆菌的离体抑菌作用最强,24 h抑菌圈直径为21.05 mm。25 mg·mL^-1浓度下,单体化合物中从组分Fr.4中分得的反式香豆酸对大肠杆菌和金黄色葡萄球菌的离体抑菌作用最强,24 h的抑菌圈直径分别为20.23 mm及18.35 mm。离体试验表明,木犀草素-6-C-洋地黄毒糖苷-4'-O-葡萄糖苷对子宫颈癌HeLa细胞、肝癌HepG2细胞及结肠癌HT-29细胞具有明显的抑制增殖作用,IC₅₀值分别为592,2 058,1 712 mg·L^-1。5 mg·kg^-1和10 mg·kg^-1的木犀草素-6-C-洋地黄毒糖苷-4'-O-葡萄糖苷对H22荷瘤鼠肝肿瘤有体内抑制作用,抑制率分别为2.9%和20.0%。【结论】苦竹叶化学成分具有一定的抑菌及抑制肿瘤增殖活性,在植物源农药及药物的开发利用方面具有一定的潜力。本研究为推进苦竹叶化学成分基础研究和促进苦竹叶资源的综合利用提供了依据。

关键词: 苦竹叶, 化学成分, 抑菌活性, 抗肿瘤活性

Abstract:

【Objective】 The compounds from leaves of Pleioblastus amarus were extracted and isolated. The inhibitive activity of the compounds against bacteria and 3 kinds of tumor cells were tested in vitro. The extract was extracted from the bamboo leaves with 95% ethanol and separated with the column chromatograph. The antibacterial activities of the extract against Escherichia coli, Staphyloccocus aureus and Bacillus subtilis were tested by the inhibition zone method. The inhibitive activity against tumor cells of 5,7,3'-trihydroxy-6-C-β-D-digitoxopyranosyl-4'-O-β-D-glucopyranosyl flavonoside was evaluated by the MTT method and the ascites tumor model of mice H22 hepatoma cells. 【Result】 The results showed that the antibacterial activity of ethyl acetate extracts was stronger than that of petroleum ether extracts and n-butanol extracts, while the water extracts had no obvious antibacterial effect. The antibacterial activity of Fr.3 was strongest in 8 fractional parts of ethyl acetate extracts. At the concentration of 25 mg·mL^-1, Fr.3 showed the strongest antibacterial activity against Bacillus subtilis, and the diameter of inhibition zone was 21.05 mm in 24 h. At the concentration of 25 mg·mL^-1, the Fr. 4 of ethyl acetate extracts had the strongest antibacterial activities against Escherichia coli and Staphyloccocus aureus of β-coumaric acid in all compounds, and the diameters of inhibition zone were 20.23 mm and 18.35 mm respectively in 24 h. High concentration of 5,7,3'-trihydroxy-6-C-β-D-digitoxopyranosyl-4'-O-β-D-glucopyranosyl flavonoside showed antitumor effects on Hela cells, HepG2 cells and HT-29 cells in vitro, and the IC₅₀was 592 mg·L^-1, 2 058 mg·L^-1and 1 712 mg·L^-1, respectively. The doses of 5 mg·kg^-1and 10 mg·kg^-15,7,3'-trihydroxy-6-C-β-D-digitoxopyranosyl-4'-O-β-D-glucopyranosyl flavonoside had antitumor effects on the liver tumors of H22 mice in vivo, and the inhibition rates were 2.9% and 20.0%, respectively. 【Conclusion】 Chemical Constituents from leaves of P. amarus have antibacterial and antitumor activities, and they have potential in the development of plant original pesticide and medicine. This study provides references for promoting the basic research and comprehensive utilization of P. amarus leaves.

Key words: Pleioblastus amarus, chemical components, antibacterial activity, antitumor activity

中图分类号:

S795.9

魏琦, 姚曦, 孙嘏, 荀航, 岳永德, 汤锋. 苦竹叶化学成分对细菌及3种肿瘤细胞的抑制活性[J]. 林业科学, 2015, 51(5): 87-94.

Wei Qi, Yao Xi, Sun Jia, Xun Hang, Yue Yongde, Tang Feng. Inhibitive Activity of Chemical Constituents from Leaves of Pleioblastus amarus against Bacteria and 3 Kinds of Tumor Cells[J]. Scientia Silvae Sinicae, 2015, 51(5): 87-94.

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