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林业科学 ›› 2001, Vol. 37 ›› Issue (2): 51-55.doi: 10.11707/j.1001-7488.20010207

• 论文及研究报告 • 上一篇    下一篇

板栗优良品种(无性系)苗木分子标记鉴别研究

翁尧富1 陈源2 赵勇春4 郑康乐3 黄少甫5 张建忠   

  1. 浙江省林业厅林木种苗站,杭州310020;中国水稻研究所,杭州310006;中国林业科学研究院亚热带林业研究所,富阳311400;余杭市长乐林场
  • 收稿日期:2000-02-14 修回日期:1900-01-01 出版日期:2001-03-25 发布日期:2001-03-25

DNA FINGERPRINTING OF CASTANEA MOLLISSIMA ELITE VARIETY (CLONE) SEEDLINGS

Weng Yaofu,Chen Yuan,Zhao Yongchun,Zheng Kangle,Huang Shaofu,Zhang Jianzhong   

  1. China National Rice Research Institute Hangzhou 310006;Subtropical Forestry Institute, CAF Fuyang 311400
  • Received:2000-02-14 Revised:1900-01-01 Online:2001-03-25 Published:2001-03-25

摘要:

利用RAPD标记对浙江省林木良种审定委员会审定(认定)和通过省级鉴定的8个板栗品种及无性系进行指纹分析,鉴别出各板栗品种(无性系)。实验过程中对684个随机寡核苷酸引物进行了重复筛选,经筛选共获得9个稳定的RAPD标记,构建了板栗的DNA指纹图谱。经过大量的实验,确定了鉴别板栗优良品种(无性系)的较为理想的实验程序,获得了RAPD良好的重复性,建立了较完整的板栗RAPD分析的技术体系,用大田苗进行初步验证,结果可靠。

关键词: 板栗, 品种(无性系)鉴别, 随机扩增多态性DNA(RAPD), DNA指纹图谱

Abstract:

RAPD——based fingerprinting was carried out for 8 varieties and clones of Castanea mollissima which had been approved by the Zhejiang Inspection Committee of Superior Tree Species.Each of the varieties (clones) was identified by fingerprints.Nine RAPD markers were obtained from repeated screening of 684 random oligonucleotide primers and DNA fingerprints were made for all the used varieties (clones).Stable amplification was obtained through modification of experimental conditions and a complete technical system was established for fingerprinting of Castanea mollissima.Preliminary results for verification of commericial seedilings showed that these fingerprints were reliable.

Key words: Castanea mollissima, Variety (clone) identification, Random amplified polymorphismic DNA, DNA fingerprinting