盐碱胁迫下金叶接骨木根系K含量模型构建

doi:10.11707/j.1001-7488.20150903

摘要/Abstract

摘要：

[目的] 以金叶接骨木为试验材料,用电阻抗图谱(EIS)法和电解质渗透率法测定根系抗盐碱性并构建其根系K含量模型,为简单快速地预测盐碱胁迫下金叶接骨木根系的K含量提供技术支持。[方法] 研究不同浓度盐碱胁迫下金叶接骨木根系EIS图谱、细胞膜透性以及K含量的变化,将根系EIS参数与相应的K含量进行线性和非线性回归分析,选取最佳的EIS参数构建估测模型,并对模型进行精度检验。[结果] 在胁迫处理期间,各浓度处理金叶接骨木根系K含量均显著低于对照(P≤0.05); 随着盐碱胁迫时间延长,各浓度处理下金叶接骨木根系的相对电导率呈现上升趋势,且均显著高于对照(P≤0.05); 随着盐碱胁迫时间延长,各浓度处理下金叶接骨木根系的电阻抗图谱变化不同; 弛豫时间τ为估测金叶接骨木根系K含量的最佳EIS参数,最佳估测模型为y =0.005 5x³-0.232 8x² +2.638 8+ 15.877,预测精度达到91.73%。[结论] 利用EIS参数构建的金叶接骨木根系K含量模型y =0.005 5x³-0.232 8x² +2.638 8x+ 15.877预测精度高,可以用于快速准确预测盐碱胁迫下金叶接骨木根系K含量的变化。

关键词: 金叶接骨木, 根系, 盐碱胁迫, 电阻抗图谱参数, K含量, 相对电导率, 模型构建

Abstract:

[Objective] Sambucus canadensis was used as experimental material in this study.To simply and fastly predict K content in roots of Sambucus canadensis under salinity and alkalinity stress, salinity and alkalinity resistance of this plant roots was measured by electrical impedance spectroscopy (EIS) and electrolyte permeability methods and the estimation model of K Content of their roots was establish. [Method] The electrical impedance spectroscopy, cell membrane permeability and the change of K content in roots during different concentrations of salinity-alkalinity stress were studied. The corrections between EIS parameters and K content were analyzed using linear and nonlinear regressions. The estimation model was constructed using the best EIS parameter and the model accuracy was tested.[Result] During the salinity and alkalinity stress,compared with control,the K content of Sambucus canadensis roots treated by different concentrations of salinity-alkalinity significantly decreased(P≤0.05). As the extension of salinity-alkalinity stress time, the relative conductivity of the roots under different stress tended to rise and significantly increased compared with control(P≤0.05). The spectra of electrical impedance changed in roots of Sambucus canadensis during salinity-alkalinity stress; The EIS parameter τ was the best for assessing K content of root. The best model was y=0.005 5x³-0.232 8x² +2.638 8x+ 15.877 with the precision accuracy of 91.73%.[Conclusion] The model y=0.005 5x³-0.232 8x² +2.638 8x+ 15.877 with best precision accuracy constructed with EIS parameter τ can be used to fastly and accurately forecast K content in roots of Sambucus canadensis under salinity and alkalinity stress.

Key words: Sambucus canadensis, root, saline-alkali stress, electrical impedance spectroscopy, K content, relative electrolytic leakage, model building

中图分类号:

S158.3

石慧勤, 张罗沙, 邸葆, 李保会, 张芹. 盐碱胁迫下金叶接骨木根系K含量模型构建[J]. 林业科学, 2015, 51(9): 18-23.

Shi Huiqin, Zhang Luosha, Di Bao, Li Baohui, Zhang Qin. Establishment of The Estimation Model of K Content of Sambucus canadensis Root under Saline-Alkali Stress[J]. Scientia Silvae Sinicae, 2015, 51(9): 18-23.

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