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林业科学 ›› 2010, Vol. 46 ›› Issue (12): 171-175.doi: 10.11707/j.1001-7488.20101228

• 研究简报 • 上一篇    下一篇

差不嘎蒿自然种群的遗传多样性与遗传分化

王铁娟, 李伟琼, 张淑艳, 孟翔飞   

  1. 内蒙古师范大学生命科学与技术学院 呼和浩特 010022
  • 收稿日期:2009-10-26 修回日期:2010-06-14 出版日期:2010-12-25 发布日期:2010-12-25

Genetic Diversity and Differentiation of Five Natural Populations of Artemisia halodendron

Wang Tiejuan, Li Weiqiong, Zhang Shuyan, Meng Xiangfei   

  1. College of Life Science and Technology, Inner Mongolia Normal University Hohhot 010022
  • Received:2009-10-26 Revised:2010-06-14 Online:2010-12-25 Published:2010-12-25

关键词: 差不嘎蒿, ISSR, 遗传多样性, 遗传分化

Abstract:

Artemisia halodendron is a dominant species of desert vegetation and an important sand-fixed plant in northeastern China. In this study, inter-simple sequence repeat (ISSR) markers were applied to investigate the genetic diversity and differentiation of five natural populations of A. halodendron . A total of 230 bands were amplified by 12 ISSR primers, of which 226 were polymorphic. Percentage of polymorphic bands (PPB) and Shannon's phenotypic diversity index ( Ho ) were respectively 98.26% and 0.329 6 at the species level,and 61.22% and 0.285 3 at the population level, showing a high level of genetic diversity. Analysis of molecular variance (AMOVA) revealed that 90.1% of the total genetic diversity occurred within populations. Based on the clustering analysis(UPGMA), the five populations were divided into two groups: the Hulunbuir Sandy Land group and the Horqin Sandy Land group. The Mantel test showed that genetic distance was significantly positively correlated with geographical distance.

Key words: Artemisia halodendron, ISSR, genetic diversity, genetic differentiation

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