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林业科学 ›› 2010, Vol. 46 ›› Issue (8): 120-124.doi: 10.11707/j.1001-7488.20100818

• 论文 • 上一篇    下一篇

美国白蛾种群的遗传多样性与遗传分化

高宝嘉1,2,杜娟1,3,高素红4,刘军侠1   

  1. 1.河北农业大学林学院 保定 071000; 2.河北北方学院 张家口 075000;3.廊坊师范学校 廊坊 065000; 4.河北科技师范学院 秦皇岛 066600
  • 收稿日期:2009-04-24 修回日期:2009-05-18 出版日期:2010-08-25 发布日期:2010-08-25

Genetic Diversity and Differentiations of Fall Webworm (Hyphantria cunea) Populations

Gao Baojia1,2;Du Juan1,3;Gao Suhong4;Liu Junxia1   

  1. 1.Forestry College, Agricultural University of Hebei Baoding 071000; 2.North University of Hebei Zhangjiakou 075000; 3.Langfang Teachers College Langfang 065000; 4.Hebei Normal University of Scicence & Technology Qinhuangdao 066600
  • Received:2009-04-24 Revised:2009-05-18 Online:2010-08-25 Published:2010-08-25

摘要:

采用微卫星技术对入侵中国的5个美国白蛾种群进行遗传多样性和遗传分化及影响因素的研究。用10对微卫星引物对300个样品DNA进行PCR扩增,并进行聚丙烯酰胺凝胶电泳分析。5个美国白蛾种群的多态位点百分率P=98.36%; 多态位点百分率的大小顺序为: 沧州种群、烟台种群、秦皇岛种群、丹东种群、石家庄种群; 美国白蛾整个群体的遗传分化: 34.38%的变异存在于种群间,65.62%的变异存在于种群内,遗传距离变化范围为0.138 6~0.322 4。用UPGMA法对Nei's遗传距离作聚类分析的结果表明: 种群的分化程度与分化时间和入侵来源密切相关。影响美国白蛾种群遗传多样性与遗传分化的主要因素是经度、平原比率、年降水量、纬度、入侵时间。美国白蛾种群现有遗传结构的形成与其生活史特性及入侵生态学特性有关。

关键词: 美国白蛾, 遗传多样性, 遗传分化, 影响因素, SSR

Abstract:

Genetic diversity,genetic differentiation and factors affected of invasive fall webworm (Hyphantria cunea) populations in China were investigated using micro-satellite technology. DNA extracted from 300 individuals were amplified using 10 SSR primers. The percentage of polymorphic loci showed that the order in the five populations was Cangzhou population,Yantai population,Qinhuangdao population,Dandong population,Shijiazhuang population. The genetic differentiation of fall webworm: 34.38%variation derived from different populations,65.62% of the variation existed within the same population,the genetic distances ranged from 0.138 6 to 0.322 4. The result of UPGMA clustering showed that population differentiation closely related to the time of differentiation and the sources of invasion. The major factors affecting the genetic diversity of fall webworm populations were longitude,the ratio of plain,annual precipitation,latitude,the invasion of time. The formation of genetic structure was correlated with characteristics of the life cycle and invasion ecology.

Key words: Hyphantria cunea, genetic diversity, genetic differentiation, factors, SSR