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林业科学 ›› 2010, Vol. 46 ›› Issue (11): 73-80.doi: 10.11707/j.1001-7488.20101110

• 论文 • 上一篇    下一篇

外源酚酸对杨树幼苗根系生理和形态发育的影响

杨阳1,2王华田1王延平1姜岳忠3王宗芹1   

  1. 1.山东农业大学农业生态环境实验室山东农业大学林学院泰安 271018; 2.山东省淄博市鲁山林场淄博 255205;3.山东省林业科学研究院济南 250014
  • 收稿日期:2010-10-09 修回日期:2010-08-24 出版日期:2010-11-25 发布日期:2010-11-25
  • 通讯作者: 王华田

Effects of Exogenous Phenolic Acids on Root Physiologic Characteristics and Morphologic Development of Poplar Hydroponic Cuttings

Yang Yang1,2Wang Huatian1Wang Yanping1Jiang Yuezhong3Wang Zongqin1   

  1. 1. Agro-Ecoenvironmental Laboratory of Shandong Agricultural UniversityForestry College of Shandong Agricultural UniversityTai’an 271018;2.Forest Park of LushanZibo 255205; 3.Shandong Academy of ForestryJinan 250014
  • Received:2010-10-09 Revised:2010-08-24 Online:2010-11-25 Published:2010-11-25

摘要:

以黑杨派欧美杨I-107 (Populus×euramericana ‘Neva’) 水培幼苗为试材,参照二代杨树人工林根际土壤中的酚酸含量(X),在改良Hoagland营养液中设置5个酚酸浓度梯度(CK,0.5X,1.0X,1.5X,2.0X),测定酚酸处理后第1,5,10,20,30和40天的根系生理生化特性和处理后第40天的根系形态数据。结果表明: 低浓度酚酸(0.5X)可提高杨树根系超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性,而高浓度酚酸(1.0X,1.5X和2.0X)则显著抑制2种酶的活性(Pa<0.05)。根系活力随酚酸浓度的提高而显著降低(Pa<0.05); 根系丙二醛含量以及根系伤害度随酚酸浓度的提高而显著提高(Pa<0.05)。酚酸能显著抑制杨树幼苗根系发育,平均根尖数、平均根长、平均根系表面积、平均根系分枝数以及平均根系分形维数显著降低,而幼苗根系直径则显著增大(Pa<0.05)。酚酸影响下,根尖附近侧根发育受到抑制,根系形状逐渐由柱形向倒金字塔形和伞形过渡,高浓度酚酸导致根尖坏死。

关键词: 酚酸, 杨树幼苗, 化感作用, 根系生理, 根系形态

Abstract:

The degradation of forestland productivity of continuous cropping poplar plantations was closely related to accumulation of phenolic acids in rhizospheric soil. Knowledge of effects of phenolic acids on physiologic characteristics and morphologic developments of poplar roots was essential to reveal the degradation mechanism of poplar forestland productivity. Hydroponic cuttings of black poplar cultivar I-107 (Populus × euramericana ‘Neva’) were cultured in Hoagland nutrient solution added with phenolic acids of five levels(CK, 0.5X, 1.0X, 1.5X, 2.0X) according to actual content in the soil of continuous poplar plantation. Physiologic indexes were studied respectively at the 1st d, 5th d, 10th d, 20th d, 30th d and 40th d after treated with phenolic acids, and data of root morphologic characteristics were obtained at the 40th d. The results showed that: Activity of superoxide dismutase(SOD) and peroxidase(POD) were both enhanced by low level phenolic acid(0.5X) and inhibited significantly by high level phenolic acids(1.0X, 1.5X, 2.0X)(Pa<0.05); and root activity reduced significantly while the level of phenolic acids rose(Pa<0.05); root malonaldehyde(MDA) content and root injury degree were both significantly enhanced by increased phenolic acids concentration (Pa<0.05). Number of root tips, mean root length, mean root area, mean root forks, mean root fractal dimension were all significantly inhibited by phenolic acids while mean root diameter was significantly promoted by phenolic acids(Pa<0.05). Lateral roots near the tip of the main root were obvious inhibited by phenolic acids. With phenolic acids level increased, the general shape of root system was changed from column aspect to inverted pyramid and to umbrella aspect . Root tips necrotized under high level phenolic acid treatments.

Key words: phenolic acids, poplar cuttings, allelopathy, root physiology, root morphology