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›› 2013, Vol. 49 ›› Issue (2): 54-60.doi: 10.11707/j.1001-7488.20130209

• 论文 • 上一篇    下一篇

核桃根系分泌物化感物质的分离与鉴定

崔翠2, 蔡靖1, 张硕新1   

  1. 1. 西北农林科技大学林学院 杨凌 712100;2. 潍坊工程职业学院园林花卉研究所 青州 262500
  • 收稿日期:2011-10-06 修回日期:2012-12-07 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 张硕新

Isolation and Identification of the Allelochemicals in Walnut (Juglans regia) Root Exudates

Cui Cui2, Cai Jing1, Zhang Shuoxin1   

  1. 1. College of Forestry, Northwest A&F University Yangling 712100;2. Research Institute of Garden and Flowers, Weifang Engineering Vocational College Qingzhou 262500
  • Received:2011-10-06 Revised:2012-12-07 Online:2013-02-25 Published:2013-02-25

摘要: 为了探讨核桃根系分泌物的化感作用,利用乙酸乙酯提取核桃活体收集到的根系分泌物并通过气相色谱和质谱联用技术(GC-MS)进行分离鉴定,研究根系分泌物对萝卜种子发芽、胚芽和胚根伸长的影响。结果表明: 根系分泌物酸性、中碱性组分对萝卜种子发芽、胚芽和胚根伸长都有抑制作用; 核桃根系分泌物酸性组分包括烷烃、烯烃、酸、酯、醇、酚、酮、胺、醌、苯等; 中性组分包括烷烃、烯烃、酸、酯、醇、酚、萜、胺、萘、醌、苯等; 碱性组分包括烷烃、烯烃、酸、醇、酚、胺、醌、酮、苯等。

关键词: 化感作用, GC-MS, 根系分泌物, 核桃

Abstract: Our objective is to investigate the allelopathic effects of walnut(Juglans regia) root exudates. The root exudates of walnut trees were extracted with ethyl acetate and were identified by using the gas chromatography-mass spectrometry (GC-MS). The impact of the exudates investigated on the seed germination and seedling growth of turnip (Brassica rapa). The results indicated that the neutral, acidic, and alkaline components of the root exudates all inhibited the seed germination and seedling growth of turnip. The acidic component mainly included alkanes, alkenes, acidic, aromatic esters, alcohols, phenol, ketones, amines, quinones, and benzene. The neutral component mainly included alkanes, alkenes, acidic, aromatic esters, alcohols, phenols, terpenes, amines, naphthalene, quinones, and benzene. The alkaline component mainly included alkanes, alkenes, acidic, alcohols, phenols, amines, quinones, ketones, and benzene. This study provids important knowledge for improving and revealing the mechanisms of allelopathy effects of walnut trees.

Key words: allelopathy, GC-MS, root exudates, walnut (Juglans regia)

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