贺兰山地区青海云杉外生菌根的形态类型及分子鉴定

doi:10.11707/j.1001-7488.20110616

林业科学 ›› 2011, Vol. 47 ›› Issue (6): 108-113.doi: 10.11707/j.1001-7488.20110616

贺兰山地区青海云杉外生菌根的形态类型及分子鉴定

樊永军¹, 闫伟², 王黎元¹

1. 包头师范学院生物科学与技术学院包头 014030;2. 内蒙古农业大学林学院呼和浩特 010018

收稿日期:2009-11-18 修回日期:2010-05-19 出版日期:2011-06-25 发布日期:2011-06-25
通讯作者: 闫伟

Morphological Type and Molecular Identification of Ectomycorrhizae on Picea crassifolia in Helan Mountain

Fan Yongjun¹, Yan Wei², Wang Liyuan¹

1. Department of Biology Science and Technology, Baotou Teachers College Baotou 014030;2. Forest College, Inner Mongolia Agricultural University Huhhot 010018

Received:2009-11-18 Revised:2010-05-19 Online:2011-06-25 Published:2011-06-25

摘要/Abstract

摘要：

从形态学、解剖学角度并利用对外生菌根真菌rDNA的ITS区段的PCR扩增产物分析,对内蒙古段贺兰山地区青海云杉外生菌根形态多样性进行初步研究,并对其外生菌根真菌物种多样性进行分子鉴定。结果表明: 1)与青海云杉共生的外生菌根有11种不同的类型; 2)经对11种不同类型的菌根真菌rDNA的ITS区段碱基序列测定并运用GenBank的序列局部相似性查询系统(BLAST)软件比对,共鉴定到种水平7株,属水平2株,科水平2株,其中子囊菌为2株,其余都为担子菌; 3)在形态、解剖学分类的基础上对外生菌根根尖进行分子鉴定,二者鉴定结果一致。

关键词: 青海云杉, 外生菌根, 形态解剖学特征, 分子鉴定

Abstract:

A preliminary study on diversity of ectomycorrhizas associated with Picea crassifolia at Helan Mountain area was investigated using morphological, anatomical and molecular characteristics, of PCR amplified product analysis of the ITS region of rDNA, and then identification results were obtained according to that the sequences were BLASTed using the GenBank. The results indicated that there were 11 different ECM types on Picea crassifolia in Helan Mountain. ITS regions of rDNA of the 11 different ECM fungi were amplified and sequenced, and the sequences were compared with those registered in DNA sequence database on Internet, identified to family level of 2, genus level of 2 and species level of 7, respectively. Among the 11 identified ectomycorrhizal types, 2 species belong to Ascomycotina and the rest species belong to Basidiomycotina. It was found that using sequences of rDNA ITS regions confirmed consistently the classification of these species based on morphological and anatomic ways.

Key words: Picea crassifolia, ectomycorrhizae, morphological and anatomical characteristics, molecular identification

中图分类号:

S718.81

樊永军;闫伟;王黎元. 贺兰山地区青海云杉外生菌根的形态类型及分子鉴定[J]. 林业科学, 2011, 47(6): 108-113.

Fan Yongjun;Yan Wei;Wang Liyuan. Morphological Type and Molecular Identification of Ectomycorrhizae on Picea crassifolia in Helan Mountain[J]. Scientia Silvae Sinicae, 2011, 47(6): 108-113.

[1]	胡兴波, 芦新建, 于洋, 贺康宁. 基于热扩散法的青海云杉冠层导度模拟[J]. 林业科学, 2018, 54(3): 8-18.
[2]	张宁, 杨云天, 王晓勤, 刘萌萌, 魏美才. 危害青海云杉的腮扁蜂属(膜翅目:扁蜂科)一新种[J]. 林业科学, 2018, 54(2): 126-130.
[3]	武星煜, 辛恒, 刘海秀, 杨启青, 魏美才. 危害青海云杉的阿扁蜂属(膜翅目:扁蜂科)一新种[J]. 林业科学, 2016, 52(9): 103-106.
[4]	张雷, 于澎涛, 王彦辉, 王顺利, 刘贤德. 祁连山北坡青海云杉中龄林生物量随海拔的变化[J]. 林业科学, 2015, 51(8): 1-7.
[5]	李倩, 吴小芹, 叶建仁. 一种马尾松菌根辅助细菌——短芽孢杆菌的筛选及鉴定[J]. 林业科学, 2015, 51(5): 159-164.
[6]	王琳, 陈展, 尚鹤. 外生菌根真菌在酸雨胁迫下对马尾松土壤微生物代谢功能的影响[J]. 林业科学, 2014, 50(7): 99-104.
[7]	欧阳芳群, 王军辉, 贾子瑞, 李悦, 仲永芳, 祁生秀. 补光对青海云杉家系幼苗生物量和矿质元素的影响[J]. 林业科学, 2014, 50(11): 188-196.
[8]	陈展, 尚鹤. 接种外生菌根菌对模拟酸雨胁迫下马尾松营养元素的影响[J]. 林业科学, 2014, 50(1): 156-163.
[9]	王琴;郭良栋. 油松外生菌根的形态解剖特征[J]. , 2013, 49(2): 100-107.
[10]	郭涛, 戴景程, 朱敏. 钾胁迫条件下Ca²⁺信号抑制剂对外生菌根真菌分泌H⁺的影响[J]. 林业科学, 2013, 49(12): 97-100.
[11]	张庆贺;马建海;赵丰钰;史全顺;王国仓. 青海云杉(拟)齿小蠹聚集信息素研究进展[J]. 林业科学, 2012, 48(6): 118-126.
[12]	王明霞;袁玲;周志峰;杨红军;黄建国. 铝对外生菌根真菌草酸分泌及氮磷钾吸收的影响[J]. 林业科学, 2012, 48(2): 82-88.
[13]	张立杰;刘鹄. 祁连山林线区域青海云杉种群对气候变化的响应[J]. 林业科学, 2012, 48(1): 18-21.
[14]	商靖;刘雪峰;阿地力·沙塔尔;潘存德;赵震宇;马红霞. 核桃基腐病的病原鉴定[J]. 林业科学, 2010, 46(12): 97-100.
[15]	李永徐启聪田国忠郭民伟朴春根. 猪屎豆丛枝病植原体的分子检测与鉴定[J]. 林业科学, 2010, 46(1): 163-168.

贺兰山地区青海云杉外生菌根的形态类型及分子鉴定

Morphological Type and Molecular Identification of Ectomycorrhizae on Picea crassifolia in Helan Mountain

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式