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林业科学 ›› 2009, Vol. 12 ›› Issue (11): 50-54.doi: 10.11707/j.1001-7488.20091109

• 论文 • 上一篇    下一篇

云杉种子超干贮藏的生理生化特征

李庆梅1 陈怀梁2 马风云2 刘艳2 张俊风3   

  1. 1.中国林业科学研究院林业研究所 国家林业局林木培育重点实验室 北京 100091; 2.山东农业大学林学院 泰安 271018; 3.北京林业大学森林培育与保护教育部重点试验室 北京 100083
  • 收稿日期:2009-07-10 修回日期:1900-01-01 出版日期:2009-11-25 发布日期:2009-11-25
  • 基金资助:
     

Effects of Ultra-Dry Treatment on Physiological and Biochemical Characteristics of Picea asperata Seeds

Li Qingmei1, Chen Huailiang2, Ma Fengyun2, Liu Yan2, Zhang Junfeng3   

  1. 1.Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry,CAF Beijing 100091;2.Forestry College of Shandong Agricultural University Taian 271018;3.Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University Beijing 100083
  • Received:2009-07-10 Revised:1900-01-01 Online:2009-11-25 Published:2009-11-25

摘要:

采用硅胶室温干燥法,云杉种子含水量由82 g·kg-1降至64,52,41,29 g·kg-1。测定不同含水量种子的发芽势、发芽率、发芽指数,并测定种子老化(50 ℃,100% RH)后的相对电导率、脱氢酶活性和异柠檬酸裂解酶活性等生理生化指标,以研究超干处理对种子活力和种子生理特性的影响。结果表明:云杉种子有较高耐脱水性,含水量降至41 g·kg-1,种子活力仍维持在较高的水平,细胞膜能够保持较好的完整性,脱氢酶活性、异柠檬酸裂解酶活性与对照没有显著差异。利用超干技术保存云杉种子是可行的。

关键词: 云杉, 种子, 超干保存, 生理生化

Abstract:

Seeds of Picea asperata were dried from 82 g·kg-1 to 64,52,41 and 29 g·kg-1 moisture content in a desiccator with silica gel. After the desiccation, seed vigor and physiological indices, including seed germination energy, germination percentage, germination index, relative conductivity, dehydrogenase activity and isocitrate lyase activity were measured. The results showed that the seeds had high desiccation tolerance.When dried to 41 g·kg-1,the seeds still had stable viability,the relatively integral cell membrane structure,the high dehydrogenase and isocitrate lyase activities.To some extent, the seed kept genetic stability by artificial aging test.

Key words: Picea asperata, seeds, ultra-drying storage, physiological and biochemical characteristics

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