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林业科学 ›› 2009, Vol. 12 ›› Issue (11): 44-49.doi: 10.11707/j.1001-7488.20091108

• 论文 • 上一篇    下一篇

花楸树天然群体的异交率*

郑健1,2 郑勇奇1 张川红1 宗亦臣1 李伯菁1   

  1. 1. 中国林业科学研究院林业研究所 北京 100091; 2. 中国科学院植物研究所北京植物园 北京 100093
  • 收稿日期:2008-08-19 修回日期:1900-01-01 出版日期:2009-11-25 发布日期:2009-11-25
  • 通讯作者: 郑勇奇
  • 基金资助:
     

Outcrossing Rates in 6 Natural Populations of Sorbus pohuashanensis

Zheng Jian1,2, Zheng Yongqi1, Zhang Chuanhong1, Zong Yichen1, Li Bojing1   

  1. 1. Research Institute of Forestry, CAF Beijing 100091;2. Beijing Botanical Garden, Resarch Institute of Botany, Chinese Academy of Sciences Beijing 100093
  • Received:2008-08-19 Revised:1900-01-01 Online:2009-11-25 Published:2009-11-25
  • Supported by:
     

摘要:

采用水平淀粉凝胶电泳技术,对采自山东、山西、河北、辽宁4个省的6个花楸树天然群体的种子样本进行分析,所用同工酶多态位点分别为Pgm-1, Pgm-2, Pgm-3,Pgi-1,Pgi-2,Pgd-1,Pgd-2,Pgd-3,利用多位点异交率估算程序(MLT)估算6个群体的异交率。结果表明: 花楸树各群体的花粉库与胚珠库中,各位点基因的配子比例差异不显著,基本处于平衡状态; 各群体多位点异交率均在0.981以上,多位点异交率与单位点异交率平均值的差值(tm-ts)显示出花楸树各群体都存在轻度的自交或近交,说明花楸树属于混合交配类型,表现为高度异交。根据电泳数据计算的近交衰退δ=1,表明花楸树天然群体的近交衰退很大。

关键词: 花楸树, 同工酶, 异交率, 近交衰退

Abstract:

Genotypes of six natural populations of Sorbus pohuashanensis were studied using horizontal starch gel electrophoresis and the mating system was analysed at eight loci(Pgm-1, Pgm-2, Pgm-3, Pgi-1, Pgi-2, Pgd-1, Pgd-2, Pgd-3) using MLT software. Results indicated that the gametes ratio of each locus in pollen and ovule pools was mainly in balance in the six populations. The multi-locus outcrossing rates were above 0.981. The margin of multi-locus minus single-locus outcrossing rate(tm-ts) showed that slight inbreeding existed in the populations. Therefore, the mating system of S. pohuashanensis in the 6 populations is predominantly outcrossing. The inferred inbreeding depression(δ=1) from the electrophoresis data indicated severe inbreeding depression in the natural populations of the species.

Key words: Sorbus pohuashanensis, isozyme marker, outcrossing rate, inbreeding depression

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