杨树部分种的AFLP遗传多样性分析

doi:10.11707/j.1001-7488.20071211

林业科学 ›› 2007, Vol. 43 ›› Issue (12): 64-69.doi: 10.11707/j.1001-7488.20071211

杨树部分种的AFLP遗传多样性分析

宋红竹¹ 张绮纹² 周春江³

1.国家林业局科技司,北京100714；2.中国林业科学研究院林业研究所,北京100091；3.中国科学院生物遗传与发育研究所,北京100101

收稿日期:2007-08-06 修回日期:1900-01-01 出版日期:2007-12-25 发布日期:2007-12-25

Analysis of Genetic Diversity of Some Species in Populus by AFLP Marker

Song Hongzhu¹,Zhang Qiwen²,Zhou Chunjiang³

1. Department of Science and Technology, State Forestry Administration Beijing 100714; 2. Research Institute of Forestry, CAF Beijing 100091;3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Beijing 100101

Received:2007-08-06 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要：

利用10对AFLP引物对杨属5个派20个种及杂种的44份杨树材料进行分析。结果表明:在这些材料中,不同引物组合的多态性均为100%,表明杨属种间及无性系间在DNA水平上存在广泛变异。利用UPGMA法对AFLP数据进行聚类分析,相似系数在0.765～0.971之间,派间聚类与经典形态分类完全一致,派内种间及种内无性系间聚类与形态分类基本相同。构建了44份材料的指纹图谱,在该图谱中,每个材料都具有自身独特的条带。最后,探讨根据分子标记结果进行杂交亲本选配及杂种子代早期选择的可行性。

关键词: 杨属, AFLP, 遗传变异, 指纹鉴定, 亲缘关系

Abstract:

Ten pairs of AFLP primers were screened out and used for the analysis of forty-four clones, which came from twenty species and hybrids of five sections in Populus. Percentage of polymorphism fragments produced by each pair of AFLP primer was 100%. All these data indicated that considerable genetic variation existed among species and clones at DNA level. Using UPGMA cluster analysis from AFLP data,molecular genetic distances among sections, species, and clones were calculated, and the relationship among them was described quantitatively. Compared with the classical taxonomy,the result of cluster analysis among sections was consistent completely, and the results of cluster analysis among intrasectional species and intraspecies clones were basically similar. We built DNA fingerprint of the 44 Populus genotypes. In this fingerprint, every plant has its own band. Finally, the feasibility of selecting and mating parents and early selection of progenies were discussed based on the results of molecular markers.

Key words: Populus, AFLP, genetic variation, fingerprints, genetic relationship

宋红竹张绮纹周春江. 杨树部分种的AFLP遗传多样性分析[J]. 林业科学, 2007, 43(12): 64-69.

Song Hongzhu;Zhang Qiwen;Zhou Chunjiang. Analysis of Genetic Diversity of Some Species in Populus by AFLP Marker[J]. Scientia Silvae Sinicae, 2007, 43(12): 64-69.

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杨树部分种的AFLP遗传多样性分析

Analysis of Genetic Diversity of Some Species in Populus by AFLP Marker

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