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林业科学 ›› 2007, Vol. 43 ›› Issue (12): 70-75.doi: 10.11707/j.1001-7488.20071212

• 论文及研究报告 • 上一篇    下一篇

不同处理对欧榛硬枝扦插生根的影响及生根过程中相关氧化酶活性的变化

扈红军1 曹帮华1 尹伟伦2 翟明普2 唐全1 贾波1   

  1. 1.山东农业大学林学院,泰安271018;2.北京林业大学资源与环境学院,北京100083
  • 收稿日期:2007-05-15 修回日期:1900-01-01 出版日期:2007-12-25 发布日期:2007-12-25

Effects of Different Treatments on Hardwood-Cutting Rooting and Related Oxidase Activity Changes during Rooting of Corylus avellana

Hu Hongjun1,Cao Banghua1,Yin Weilun2,Zhai Mingpu2,Tang Quan1,Jia Bo1   

  1. 1.Forestry College of Shandong Agricultural University Tai'an 271018; 2.Environment and Resources College of Beijing Forestry University Beijing 100083
  • Received:2007-05-15 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

摘要:

以欧榛品种巴塞罗娜为材料,进行基质和生长素的对比试验,研究生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)酶活性的动态变化。结果表明:在基质试验中,只有混合基质能够提高插穗生根率;在生长素试验中,IBA处理的插穗生根率达到60%,α-NAA和ABT1处理的插穗生根率只有15%和20%,而对照不生根。榛子硬枝扦插生根过程可分为诱导期、表达期和伸长期3个阶段。POD活性在诱导期和表达期呈上升趋势,伸长期下降,在第20天和60天各有1个高峰;PPO活性在诱导期和表达期上升,伸长期下降;IAAO活性在诱导期缓慢上升,表达期急剧上升,伸长期下降。

关键词: 欧榛, 硬枝扦插, 生根, 氧化酶活性

Abstract:

Cuttings propagation technology and related oxidase changes of Corylus avellana Barcelona were studied with media and auxin comparison experiments. The dynamic change of activities of peroxidase (POD), polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO) during rooting were also investigated. The results showed that only the mixed media can enhance the percentage of cuttings rooting in the media experiment. Rooting percentage of cutting with 200×10-6 IBA treatment was 60%, rooting percentage of cutting with 200×10-6 α-NAA or ABT1 treatment was 15% and 20%, the cuttings of control did not root. The adventitious rooting displayed three distinct phases I.e. induction, expression and elongation. The peroxidase activity in IBA-treated cuttings increased during induction and expression phase declined in the elongation phase. There were two peaks of the POD activity respectively on the 20th and 60th during rooting, Polyphenol oxidase activity in IBA-treated cuttings increased during induction phase, and then declined slowly during expression and elongation phases. Indoleacetic acid oxidase activity of IBA-treated cuttings increased slowly during induction phase and then increased sharply during expression phases and decreased during elongation phases.

Key words: Corylus avellana, hardwood cutting, rooting, oxidases