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林业科学 ›› 2007, Vol. 43 ›› Issue (02): 43-47.doi: 10.11707/j.1001-7488.20070208

• 论文及研究报告 • 上一篇    下一篇

SSR标记与欧洲黑杨材性性状的关联分析

黄秦军 丁明明 张香华 苏晓华   

  1. 中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091
  • 收稿日期:2006-07-26 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Association Analysis Between SSR Molecular Markers and Wood Properties of Populus nigra

Huang Qinjun Ding Mingming Zhang Xianghua Su Xiaohua   

  1. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091
  • Received:2006-07-26 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要:

对引进的92个1年生和65个4年生欧洲黑杨无性系进行材性测定,并结合171个SSR标记进行材性数量性状位点的关联分析。结果表明:1年生和4年生欧洲黑杨的木材基本密度分别为0.3088g.cm-3和0.3552g.cm-3,纤维长分别为601.3μm和649.4μm,纤维宽分别为21.81μm和23.11μm,微纤丝角分别为26.5°和26.1°。4年生欧洲黑杨苯醇抽提物、综纤维素、α纤维素以及木质素含量分别为2.32%、78.62%、41.48%和20.93%。通过SSR标记位点与性状间的关联分析,共获得与1年生材性性状关联的SSR标记位点10个,其中基本密度3个,微纤丝角5个,纤维长2个,纤维宽4个,贡献率6.43%~23.18%;与4年生欧洲黑杨材性性状关联的SSR标记位点8个,其中基本密度、纤维长、木质素各1个,微纤丝角、综纤维素含量和α纤维素含量各2个,贡献率7.41%~28.56%。

关键词: 欧洲黑杨, SSR标记, 材性性状

Abstract:

In this study, the wood properties of 92 one-year-old and 65 four-year-old Populus nigra clones were measured. QTLs analysis was combined with the measured result with 171 SSR marker loci, and the research showed that the wood basic density (WBD) of one-year-old and four-year-old was 0.308 8 g·cm-3 and 0.355 2 g·cm-3 , the fibre length(FL) was 601.3 μm and 649.4 μm, the fibre width(FW) was 21.81 μm and 23.11 μm and the microfibre angle(MFA) was 26.5° and 26.1° respectively. The contents of benzene-alcohol extraction, holocellulose, α-cellulose and lignin of four-year-old P. nigra were 2.32%, 78.62%, 41.48% and 20.93% respectively. SSR marker loci correlated with WBD, MFA, FL and FW of one-year-old P. nigra were identified to be 3,5,2 and 4 respectively, and their contributions ranged from 6.43% to 23.18%. In four-year-old P. nigra, SSR marker loci correlated with WBD, FL, MFA, holocellulose, α-cellulose and lignin were identified to be 1, 1, 2, 2, 2 and 1 respectively, and the contributions ranged from 7.41% to 28.56%. These markers might be useful for wood property improvement if using them correctly in breeding program.

Key words: Populus nigra, SSR marker, wood property