欧洲黑杨优质核心种质库的初步构建

doi:10.11707/j.1001-7488.20140907

摘要/Abstract

摘要：

对159 份欧洲黑杨种质资源的生长、育种值、材性、养分利用率和水分利用率等 13 个性状进行分析,采用 13 种表型选优的抽样方法构建欧洲黑杨优质核心种质库。表型值评价表明:当抽样群体比例达到 21.4%以上即能满足构建核心种质要求,而只有抽样群体比例达到30.2%以上SSR分子遗传多样性才能满足构建核心种质要求。最终确定的欧洲黑杨优质核心种质库包含48份种质资源,占原群体的30.2%；与原群体相比,均值和方差不存在显著差异,极差符合率和变异系数变化率分别为 97.07%和89.72%；优质核心种质库的有效等位基因数、Nei's遗传多样性系数和 Shannon's 信息指数与原群体之间没有显著差异,保持了原群体的遗传多样性。

关键词: 欧洲黑杨, 优质核心种质, 抽样方法, 遗传多样性

Abstract:

In this study, we analyzed 13 traits of 159 Populus nigra germplasm such as growth, breeding value, material properties, efficiency of water use efficiency and efficiency of nutrient utilization. A high-quality core collection of P. nigra was constructed by utilizing 13 kinds of sampling methods of phenotypic selection. Phenotypic value evaluation showed that the sampling rate reached to more than 21.4% can meet the requirement of building core collection. However, the proportion of the sample population over 30.2% was required for building the core collection in terms of SSR molecular genetic diversity. Ultimately, the determined high-quality core collection of P. nigra contained 48 germplasm resources, accounting for 30.2% of the original germplasm. Compared with the original germplasm, there were no differences in the aspects of the mean and the variance, and the coincidence rate of range and the changeable rate of coefficient of variation were 97.07% and 89.72%. The high-quality core collection was no significantly different from the original group in the aspects of the effective number of alleles, Nei’s genetic diversity coefficient and Shannon's information index, suggesting that the high-quality core collection should maintain the genetic diversity of the original group.

Key words: Populus nigra, high-quality core collection, sampling method, genetic diversity

中图分类号:

S722.8

曾宪君, 李丹, 胡彦鹏, 黄秦军, 苏晓华. 欧洲黑杨优质核心种质库的初步构建[J]. 林业科学, 2014, 50(9): 51-58.

Zeng Xianjun, Li Dan, Hu Yanpeng, Huang Qinjun, Su Xiaohua. A Preliminary Study on Construction of High-Quality Core Collection of Populus nigra[J]. Scientia Silvae Sinicae, 2014, 50(9): 51-58.

参考文献

陈亮,杨亚军,虞富莲.2004.中国茶树种质资源研究的主要进展和展望.植物遗传资源学报,5(4): 389-392.
(Chen L, Yang Y J, Yu F L. 2004. Tea Germplasm research in China: recent progresses and prospects. Journal of Plant Genetic Resources,5(4): 389-392. [in Chinese] )
崔艳华,邱丽娟,常汝镇,等.2004.黄淮夏大豆(G.max)初选核心种质代表性检测. 作物学报,30(3): 284-288.
(Cui Y H, Qiu L J, Chang R Z, et al. 2004. Representative test for primary core collection of summer sowing soybeans in Huanghuai region of China. Acta Agronomica Sinica,30(3): 284-288. [in Chinese] )
丁明明,苏晓华,黄秦军.2006.欧洲黑杨基因资源稳定碳同位素组成特征.林业科学研究, 19(3): 272-276.
(Ding M M, Su X H, Huang Q J. 2006. Study on Stable carbon isotopic composition of Populus nigra L.Gene resource. Forest Research, 19(3): 272-276. [in Chinese] )
丁明明,黄秦军,苏晓华.2008.欧洲黑杨基因资源材性关联基因的SNP分析.遗传,30(6): 795-800.
(Ding M M, Huang Q J, Su X H. 2008. Analysis on SNPs linked with wood properties of Populus nigra L.gene resources. Hereditas,30(6): 795-800. [in Chinese] )
董英山.2000.中国野生大豆遗传多样性及核心种质构建.长春: 东北师范大学博士学位论文.
高书燕,董文轩,梁敏.2011.辽宁省山楂资源微核心种质的构建方法和评价.中国果树,(5): 14-20.
李秀兰,贾继文,王军辉,等.2013.灰楸形态多样性分析及核心种质初步构建.植物遗传资源学报,14(2): 243-248.
(Li X L, Jia J W, Wang J H, et al. 2013. Morphological diversity analysis and preliminary construction of core collection of Catalpa fargesii bureau. Journal of Plant Genetic Resources,14(2): 243-248. [in Chinese] )
李自超,张洪亮,孙传清,等.1999.植物遗传资源核心种质研究现状与展望.中国农业大学学报,4(5): 51-62.
(Li Z C, Zhang H L, Sun C Q, et al. 1999. Status and prospects of core collection in plant Germplasm resource. Journal of China Agricultural University,4(5): 51-62. [in Chinese] )
李自超,张洪亮,曾亚文,等.2000.云南地方稻种资源核心种质取样方法研究.中国农业科学,33(5): 1-7.
(Li Z C, Zhang H L, Zeng Y W, et al. 2000. Study on sampling schemes of core collection of local varieties of rice in Yunnan, China. Scientia Agricultura Sinica,33(5): 1-7. [in Chinese] )
李自超,张洪亮,曹永生. 2003.中国地方稻种资源初级核心种质取样策略研究,作物学报,(1): 20-24.
(Li Z C, Zhang H L, Cao Y S. 2003. Studies on the sampling strategy for primary core collection of chinese ingenious rice. Acta Agronomica Sinica,(1): 20-24. [in Chinese] )
刘希华,丁昌俊,张伟溪,等.2010.不同基因型欧洲黑杨幼苗氮素利用效率差异及其机理初探.林业科学研究,23(3): 368-374.
(Liu X H, Ding C J, Zhang W X, et al. 2010. Study on difference and primarily mechanism of nitrogen use efficiency(NUE) in Populus nigra seedlings genotypes. Forest Research,23(3): 368-374. [in Chinese] )
明军,张启翔,兰彦平.2005.梅花品种资源核心种质构建.北京林业大学学报,27(2): 65-69.
(Ming J, Zhang Q X, Lan Y P. 2005. Core collection of Prunus mume Sieb. et Zucc. Journal of Beijing Forestry University,27(2): 65-69. [in Chinese] )
倪茂磊.2011.美洲黑杨遗传多样性分析与核心种质库构建.南京: 南京林业大学硕士学位论文.
(Ni M L. 2011. Analysis of genetic diversity and construction of primary core collection of Populu deltoids. Nanjing: MS thesis of Nanjing Forestry University. [in Chinese] )
邱丽娟,曹永生,常汝镇,等.2004.中国大豆(Glycine max)核心种质构建 I.取样方法研究.中国农业科学,36(12): 1442-1449.
(Qiu L J, Cao Y S, Chang R Z, et al. 2004. Establishment of Chinese Soybean (G.max) Core Collection Ⅰ. Sampling Strategy. Scientia Agricultura Sinica,36(12): 1442-1449. [in Chinese] )
沈进,朱立武,张水明,等.2008.中国石榴核心种质的初步构建.中国农学通报,24(5): 265-271.
(Shen J, Zhu L W, Zhang S M, et al. 2008. The construction of primary core collection of pomegranate (Punica granatum L.) Germplasm resources cultivated in China. Chinese Agricultural Science Bulletin,24(5): 265-271. [in Chinese] )
孙传清,李自超,王象坤.2001.普通野生稻和亚洲栽培稻核心种质遗传多样性的检测研究.作物学报,27(3): 313-318.
(Sun C Q, Li Z C, Wang X K. 2001. Studies on evaluation of the genetic diversity of core collection of common wild rice (Oryza rufipogon Griff.) and asian cultivated rice (Oryza sativa L.). Acta Agronomica Sinica,27(3): 313-318. [in Chinese] )
王新超,杨亚军,陈亮,等.2004.构建茶树资源核心种质的设想.亚热带植物科学,33(4): 52-56.
(Wang X C, Yang Y J, Chen L, et al. 2004. Establishment of core collection in tea Germplasm. Subtropical Plant Science,33(4): 52-56. [in Chinese] )
魏志刚,高玉池,刘桂丰,等.2009.白桦核心种质初步构建.林业科学,45(10): 74-80.
(Wei Z G, Gao Y C, Liu G F, et al. 2009. Preliminary construction of core collection of Betula platyphylla Germplasm. Scientia Silvae Sinicae,45(10): 74-80. [in Chinese] )
徐海明,李晓玲,李金泉,等.2005.整合质量数量性状构建作物核心种质的策略研究.浙江大学学报: 农业与生命科学版,31(4): 362-367.
(Xu H M, Li X L, Li J Q, et al. 2005. Study on the strategies of integrating qualitative and quantitative traits for constructing core collection. Journal of Zhejiang University: Agric.& Life Sci.,31(4): 362-367. [in Chinese] )
余萍,李自超,张洪亮,等.2003.中国普通野生稻初级核心种质取样策略.中国农业大学学报,8(5): 37-41.
(Yu P, Li Z C, Zhang H L, et al. 2003. Sampling strategy of primary core collection of common wild rice (Oryza rufipogon Griff.) in China. Journal of China Agricultural University,8(5): 37-41. [in Chinese] )
袁海涛,董玉芝,王肇延.2012.用最小距离逐步取样法构建野核桃核心种质.浙江农业科学,(7): 972-974.
张洪亮,李自超,曹永生,等.2003.表型水平上检验水稻核心种质的参数比较.作物学报,29(2): 252-257.
(Zhang H L, Li Z C, Cao Y S, et al. 2003. Comparison of parameters for testing the rice core collection in phenotype. Acta Agronomica Sinica,29(2): 252-257. [in Chinese] )
张维瑞,袁王俊,尚富德.2012.基于AFLP分子标记的桂花品种核心种质的构建.西北植物学报,32(7): 1349-1354.
(Zhang W R, Yuan W J, Shang F D. 2012. Development of core collection of Osmanthus fragrans Lour. cultivars based on AFLP molecular Markers. Acta Botanica Boreali-Occidentalia Sinica,32(7): 1349-1354. [in Chinese] )
张香华,苏晓华,黄秦军,等.2006.欧洲黑杨育种基因资源SSR多态性比较研究.林业科学研究, 19(4): 477-483.
(Zhang X H, Su X H, Huang Q J, et al. 2006. Comporison of genetic variations of black poplar (Populus nigra L.) gene resource by microsatellite markers. Forest Research, 19(4): 477-483. [in Chinese] )
Brown A H D.1989.Core collections: a practical approach to genetic resources management.Genome,31(2): 818-824.
Diwan N,Mclntosh M S,Bauchan G R.1995.Methods of developing a core collection of annual Medicago species. Theoretical and Applied Genetics,90(6): 755-761.
El Mousadik A,Petit R J.1996.High level of genetic differentiation for allelic richness among populations of the argan tree(Argania spinosa(L.) Skeels)endemic to Morocco.Theoretical and Applied Genetics,92(7): 832-839.
FAO.1996.Report on the state of world’s plant genetic resources for food and agriculture.Prepared for the International Technical Conference on Plant Genetic Resources.Leipzig,Germany.
FAO.2010.The second report on the state of world’s plant genetic resources for food and agriculture.FAO,Rome.
Frankel O H,Brown A H D.1984.Plant genetic resources today: a critical appraisal//Genetics: New Frontiers(Vol.Ⅳ).New Delhi,India: Oxford and IBH Publishing.
Hu J, Zhu J, Xu H M.2000.Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops.Theoretical and Applied Genetics,101(1/2): 264-268.
Igartua E,Gracia M P,Lasa J M, et al.1998.The Spanish barley core collection.Genetic Resources and Crop Evolution,45(5): 475-481.
Kimura M,Crow J F.1964.The number of alleles that can be maintained in a finite population.Genetics,49: 725-738.
Petit R J,El Mousadik A,Pons O.1998.Identifying populations for conservation on the basis of genetic markers.Conservation Biology,12(4): 844-855.
Thachuk C,Crossa J,Franco J,et al.2009.Core hunter: an algorithm for sampling genetic resources based on multiple genetic measures.BMC Bioinformatics,10(1): 243.
Upadhyaya H D,Ortiz R.2001.A mini core subset for capturing diversity and promoting utilization of chickpea genetic resources in crop improvement.Theoretical and Applied Genetics,102(8): 1292-1298.
Vanden B A.2003.EUFORGEN technical guidelines for genetic conservation and use for European black poplar(Populus nigra L.).International Plant Genetic Resources Institute, Rome,1-6.
Yonezawa K,Nomura T,Morishima H.1995.Sampling strategies for use in stratified germplasm collections//Hodgkin T,Brown A H D,van Hintum T H L,et al.Core collections of plant genetic resources.International Plant Genetic Resoursces Institute(IPGRI): A Wiley-Sayce Publication,35-54.

[1]	Shahzad Muhammad, 韩斐斐, 姜立春. 不同抽样方法对兴安落叶松立木材积方程预测精度的影响[J]. 林业科学, 2018, 54(8): 99-105.
[2]	金玲, 刘明国, 董胜君, 吴月亮, 张欣. 97个山杏无性系的遗传多样性及SSR指纹图谱[J]. 林业科学, 2018, 54(7): 51-61.
[3]	包文泉, 乌云塔娜, 杜红岩, 李铁柱, 刘慧敏, 王淋, 白玉娥. 基于SSR标记的西藏光核桃群体遗传多样性和遗传结构分析[J]. 林业科学, 2018, 54(2): 30-41.
[4]	杨汉波, 张蕊, 王帮顺, 徐肇友, 陈焕伟, 周志春. 木荷优树无性系种质SSR标记的遗传多样性分析[J]. 林业科学, 2017, 53(5): 43-53.
[5]	毛秀红, 郑勇奇, 孙百友, 张元帅, 韩丛聪, 位晓, 荀守华. 基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建[J]. 林业科学, 2017, 53(10): 80-89.
[6]	冯源恒, 李火根, 杨章旗, 黄永利, 罗群凤, 张远. 广西马尾松第2代育种群体的组建[J]. 林业科学, 2017, 53(1): 54-61.
[7]	宋志姣, 杨合宇, 翁启杰, 周长品, 李发根, 李梅, 卢万鸿, 罗建中, 甘四明. 细叶桉群体的遗传多样性和受选择位点[J]. 林业科学, 2016, 52(9): 39-47.
[8]	廖柏勇, 王芳, 陈丽君, 刘明骞, 欧阳昆唏, 阙青敏, 惠文凯, 李培, 陈晓阳. 基于SRAP分子标记的苦楝种质资源遗传多样性分析[J]. 林业科学, 2016, 52(4): 48-58.
[9]	杨汉波, 张蕊, 周志春. 木荷种子园的遗传多样性和交配系统[J]. 林业科学, 2016, 52(12): 66-73.
[10]	李培, 阙青敏, 欧阳昆唏, 李俊成, 湛欣, 朱芹, 张俊杰, 邓小梅, 陈晓阳. 不同种源红椿SRAP标记的遗传多样性分析[J]. 林业科学, 2016, 52(1): 62-70.
[11]	张振, 张含国, 莫迟, 张磊. 红松转录组SSR分析及EST-SSR标记开发[J]. 林业科学, 2015, 51(8): 114-120.
[12]	梁玉琴, 张嘉嘉, 梁晋军, 李芳东, 韩卫娟, 傅建敏. 河南省柿种质资源的遗传多样性[J]. 林业科学, 2015, 51(6): 71-80.
[13]	占明明, 杨毅, 程子彰, 苏光灿, 胡伟, 陈华萍, 黄乾明. 基于SRAP标记的油橄榄品种遗传多样性分析[J]. 林业科学, 2015, 51(1): 157-164.
[14]	刘政捷, 林源, 周明兵, 汤定钦. 应用微卫星分子标记估算毛竹的异交率[J]. 林业科学, 2014, 50(8): 76-81.
[15]	赵亚琦, 成铁龙, 施季森, 王新民, 徐阳, 刘伟东, 陈金慧. 鹅掌楸属SRAP分子标记体系优化及遗传多样性分析[J]. 林业科学, 2014, 50(7): 37-43.