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林业科学 ›› 2009, Vol. 12 ›› Issue (6): 22-26.doi: 10.11707/j.1001-7488.20090605

• 论文 • 上一篇    下一篇

马尾松实生种子园交配系统分析*

张薇 龚佳 季孔庶   

  1. (南京林业大学 林木遗传与生物技术省部共建教育部重点实验室 南京210037)
  • 收稿日期:2008-04-25 修回日期:1900-01-01 出版日期:2009-06-25 发布日期:2009-06-25
  • 通讯作者: 季孔庶

Analyses on the Mating System in Seedling Seed Orchard of Pinus massoniana

Zhang Wei,Gong Jia,Ji Kongshu   

  1. (Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037)
  • Received:2008-04-25 Revised:1900-01-01 Online:2009-06-25 Published:2009-06-25

摘要:

以福建白砂林场马尾松实生种子园自由授粉子代为研究对象,从215对微卫星(SSR)引物中筛选出6对多态性比较理想者作为分子标记对种子园进行交配系统分析。结果表明:1)该种子园的异交率较高,多位点异交率2006和2007年分别为1.200(标准差SD=0.000)和1.072(SD=0.066); 年度间异交率相似。2)不同冠层和方位上的异交率大小为:上层>中层>下层; 东面<南面=西面=北面,与其花量的调查结果相吻合。3)从坡向来看,种子园西坡与北坡的异交率无显著差异。

关键词: 马尾松, 实生种子园, 交配系统, SSR标记

Abstract:

A mating system of the seedling seed orchard of Pinus massoniana in Baisha Forest Farm,Fujian Province, was studied by microsatellite (SSR) markers. Six SSR primers selected from 215 primers were used for the mating system analysis. The results were as follows: 1) The outcrossing rate of the seed orchard was very high. The multilocus outcrossing rate was similar between 2006 and 2007, and was respectively 1.200(standard deviation, SD=0.000) and 1.072 (SD=0.066). 2)The outcrossing rate in the top canopy of the crown was higher than those from the middle and bottom position of the crown. Eastside of the crown had a lower outcrossing rate than the other sides. 3) There was not significant difference in the north and west hillside in the seed orchard.

Key words: Pinus massoniana, seedling seed orchard, mating system, SSR marker