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林业科学 ›› 2007, Vol. 43 ›› Issue (02): 37-42.doi: 10.11707/j.1001-7488.20070207

• 论文及研究报告 • 上一篇    下一篇

次氯酸钠水浴处理对板栗呼吸和几种酶活性的影响

杨小胡1,2 梁丽松1 王贵禧1 石雪晖3   

  1. 1.中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091;2.湖南省林业科学院长沙410004;3.湖南农业大学园艺园林学院,长沙410128
  • 收稿日期:2006-05-09 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Effects of Sodium Hypochlorite Solution Water-Curing Treatment on Respiration and Amylase Activity of Chestnut

Yang Xiaohu1,2 Liang Lisong1 Wang Guixi1 Shi Xuehui3   

  1. 1. The Research Institute of Forestry, The Chinese Academy of Forestry Key Laboratory of Forest Silviculture of State Forestry Administration Beijing 100091;2. Hunan Forestry Academy Changsha 410004; 3. Horticulture and Landscape College of Hunan Agriculture University Changsha 410128
  • Received:2006-05-09 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要:

以‘燕昌’板栗为试材,研究采后不同浓度NaClO水浴处理对板栗贮藏生理代谢的影响。结果表明:NaClO水浴处理使板栗呼吸强度和淀粉酶活性降低,乙醇脱氢酶(ADH)活性升高;NaClO水浴处理使板栗内部PPO活性降低,对外部PPO活性的影响与NaClO浓度相关,1000mg·L-1NaClO处理使板栗外部PPO活性一直高于对照,而100mg·L-1NaClO和500mg·L-1NaClO处理使外部PPO活性降低;NaClO水浴处理使板栗内部POD活性降低,对外部POD活性的影响与NaClO浓度相关,1000mg·L-1NaClO处理使板栗外部POD活性增加,其他处理在贮藏前期降低了外部POD活性,但在后期使外部POD活性升高;100mg·L-1NaClO和500mg·L-1NaClO处理使板栗SOD活性升高,而1000mg·L-1NaClO处理则使SOD活性降低;NaClO处理板栗的CAT活性变化趋势与对照相似,但提高了CAT活性。

关键词: 板栗, 次氯酸钠, 水浴处理, 呼吸强度, 酶活性

Abstract:

In this paper, the effects of different concentrations (100, 500, 1 000 mg·L-1 ) of sodium hypochlorite solution water-curing treatment on physiological changes of chestnut (Castanea mollissima cv. Yanchang)were studied during postharvest MA storage. The results were as follows: 1) NaClO water-curing treatment could decrease respiratory intensity and amylase activity, but increase ADH activity of chestnut; 2) NaClO water-curing treatment could inhibit intra-PPO activity, extra-PPO activity was related with concentrations of NaClO solution. The extra-PPO activity of chestnut treated with 1 000 mg·L-1 NaClO solution was higher than that of CK at all times. However, 100 mg·L-1 or 500 mg·L-1 NaClO solution water-curing treatment made extra-PPO activity lower than CK. 3) NaClO water-curing treatment could restrain intra-POD activity, extra-POD activity was related with concentrations of NaClO solution. Extra-POD activity of chestnut treated with 1 000 mg·L-1 NaClO solution was higher than that of CK. However, extra-POD activity was lower in the early storage and higher in the later treated with 100 mg·L-1 or 500 mg·L-1 NaClO solution. 4) SOD activities were higher than CK by 100 mg·L-1 NaClO or 500 mg·L-1 NaClO solution treatment, but SOD activity was lower than CK by 1 000 mg·L-1 NaClO solution treatment. 5) There were no differences between NaClO water-curing treatments and CK on the changing trends of CAT activities, but CAT activity was improved by NaClO water-curing treatment.

Key words: chestnut, sodium hypochlorite, water_curing treatment, respiratory intensity, enzyme activity