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›› 2013, Vol. 49 ›› Issue (2): 127-133.doi: 10.11707/j.1001-7488.20130219

• 研究简报 • 上一篇    下一篇

桉树Genomic-SSR和EST-SSR引物的快速筛选与通用性研究

刘果1, 张党权2, 谢耀坚1, 谷振军2, 章怀云2   

  1. 1. 国家林业局桉树研究开发中心 湛江 524022;2. 中南林业科技大学 林业生物技术湖南省重点实验室 经济林育种与栽培国家林业局重点实验室 经济林培育与保护省部共建教育部重点实验室 长沙 410004
  • 收稿日期:2012-07-05 修回日期:2012-08-27 出版日期:2013-02-25 发布日期:2013-02-25
  • 通讯作者: 谢耀坚

Rapid Screening and Transferability Analysis of Genomic-SSR and EST-SSR Primers in Eucalypt

Liu Guo1, Zhang Dangquan2, Xie Yaojian1, Gu Zhenjun2, Zhang Huaiyun2   

  1. 1. China Eucalypt Research Centre Zhanjiang 524022;2. Hunan Provincial Key Laboratory of Forestry Biotechnology Key Laboratory of Non-Wood Forest Products of State Forestry Administration Key Laboratory of Non-Wood Forest Trees and Protection of Ministry of Education Central South University of Forestry and Technology Changsha 410004
  • Received:2012-07-05 Revised:2012-08-27 Online:2013-02-25 Published:2013-02-25

关键词: SSR标记, 桉树, 引物筛选, 基因组DNA混合池

Abstract: Based on the relevant properties of EST-SSR gene function, and the high polymorphism and potential regulatory function of Genomic-SSR, the polymorphism effect and transferability of EST-SSR and genomic-SSR of eucalypt trees was comparatively studied in this article. A genomic DNA pooling method was developed to rapidly screen eucalypt SSR primers. A total of 340 pairs of eucalypt SSR primers were screened from 395 pairs designed by optimizing SSR-PCR system, and the screening ratio was 86.06%. Genomic-SSR and EST-SSR screened 204 pairs (ratio 83.26%) and 136 pairs (ratio 90.67%), respectively. The result indicates that these obtained SSR primers have good polymorphism and transferability in eucalypt trees, and are suitable for SSR marker analysis of various eucalypt species. Compared with the conventional single-template screening method, the first round of screening primers by genomic DNA pooling technology has reduced more than 82% of workload and reagent consumption. The findings lay a foundation for all-aspect, fast and accurate application of SSR marker technology in eucalypt trees.

Key words: SSR marker, Eucalyptus, primer screening, genomic DNA pooling

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