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林业科学 ›› 2006, Vol. 42 ›› Issue (6): 37-39.doi: 10.11707/j.1001-7488.20060607

• 论文及研究报告 • 上一篇    下一篇

香樟未成熟合子胚体胚发生及植株再生研究

杜丽1,2 叶要妹1 包满珠1   

  1. 1.华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,武汉430070;2.南阳师范学院中英南阳洛桑昆虫生物学联合实验室南阳473000
  • 收稿日期:2004-11-19 修回日期:1900-01-01 出版日期:2006-06-25 发布日期:2006-06-25

Study on Somatic Embryogenesis and Plant Regeneration of Immature Zygotic Embryo of Cinnamomum camphora

Du Li1,2,Ye Yaomei1,Bao Manzhu1   

  1. 1.Key Lab of Horticultural Plant Biology of Ministry of Education College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan 430070;2.China-UK Nanyang Normal University_Rothamsted Research Joint Lab of Insect Biology Nanyang Normal University Nanyang 473000
  • Received:2004-11-19 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

摘要:

以香樟的未成熟幼胚为外植体进行诱导体胚发生影响因素的研究。成功地从香樟幼胚中诱导出胚状体并获得再生植株,MS为基本培养基,蔗糖是最佳的碳源。经2年对初生胚的继代培养,获得胚性愈伤组织;胚性愈伤组织经体胚诱导、体胚成熟、体胚萌发3个过程的诱导后能够获得再生植株。

关键词: 香樟, 未成熟合子胚, 胚状体, 胚性愈伤组织, 植株再生

Abstract:

Somatic embryogenesis was successfully induced from immature embryos of Cinnamomum camphora. The best carbohydrate form was sucrose under the condition of MS+1.0 mg·L-1 BA + 0.1 mg·L-1 2,4-D + 700 mg·L-1 CH. After subculture of 2 years, embryogenic calli were observed on the somatic embryos. Embryogenic calli were transferred onto induction medium for somatic embryos initiation, maturation medium for somatic embryo maturing, and then to germination medium for shoot induction. The embryogenic calli could be induced to develop into plantlet and successfully transferred to outdoor environment.

Key words: Cinnamomum camphora, immature zygotic embryo, somatic embryo, embryogenic calli, plant regeneration