关键词: 白皮松, 等位酶, 遗传多样性, 保护策略

Abstract:

Ten populations with 210 families of Pinus bungeana were collected in its natural stands by delamination sampling method. Sixteen enzyme systems had been analyzed and 31 loci were measured. The results showed that the genetic diversity of P. bungeana was slight lower than the average of Pinus species. The parameters of genetic diversity of P. bungeana at species level were given as follows: mean number of alleles per locus A_S=1.742, mean number of effective alleles per locus Ae_S=1.49, percentage of polymorphic loci P_S=54.8%, Hardy-weinberg expected heterozygosity He_S=0.162. The parameters of genetic diversity of P. bungeana at population level were given as follows: A_P=1.39±0.11, Ae_P=1.30±0.12, P_P=34.85%±8.46%, He_P=0.099±0.049,directly counted mean heterozygosity Ho_P=0.095±0.042. The genetic differentiation among populations of P. bungeana was obvious(F_ST=0.133). The genetic distance between populations was large(D=0.128). Ten populations were divided into three groups according to Nei's genetic identity. The genetic diversity of natural population of P. bungeana showed as central distribution, which was accordant with its natural resources distribution and its morphology diversity distribution, and its implication for conservation strategy was discussed.

Key words: Pinus bungeana, allozyme, genetic diversity, conservation strategy