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林业科学 ›› 2005, Vol. 41 ›› Issue (1): 42-48.doi: 10.11707/j.1001-7488.20050109

• 论文及研究报告 • 上一篇    下一篇

毛新杨×毛白杨叶片表型和春季萌芽时间QTL分析(英文)

张德强 张志毅 杨凯 李百炼   

  1. 1.北京林业大学毛白杨研究所,北京100083;2.中国农业科学院品种资源研究所,北京100081;3.美国北卡罗莱纳州立大学林学系,北卡罗莱纳州NC27695-8203
  • 收稿日期:2004-04-16 修回日期:1900-01-01 出版日期:2005-01-25 发布日期:2005-01-25

QTL Analysis of Leaf Morphology and Spring Bud Flush in (Populus tomentosa×P. bolleanaP. tomentosa

Zhang Deqiang1,Zhang Zhiyi1,Yang Kai2,Li Bailian3   

  1. 1.Institute of Populus tomentosa, Beijing Forestry University Beijing100083; 2.Institute of Crop Germplasm, Chinese Academy of Agriculture Beijing100081; 3.Department of Forestry, North Carolina State University North Carolina State27695-8203
  • Received:2004-04-16 Revised:1900-01-01 Online:2005-01-25 Published:2005-01-25

摘要:

以毛新杨无性系TB0 1×毛白杨无性系LM50的回交子代120株个体为作图群体,对控制叶片表型性状如叶长、叶宽、叶面积和叶柄长以及春季萌芽时间共5个性状的数量性状位点(quantitativetraitloci,QTLs)进行分析。运用AFLP标记技术结合拟测交作图策略构建了含有393个AFLP标记的毛白杨及毛新杨的遗传连锁框架图。毛白杨遗传图上共含有247个AFLP标记位点,连锁位点覆盖毛白杨基因组总长约3265.1cM ,而毛新杨遗传连锁图上共含有146个标记位点,连锁位点覆盖毛新杨基因组总长约1992cM ,这些连锁图的每一连锁群上含有的标记数为4~30个。在此基础上,利用区间作图软件共检测到控制叶片表型性状的QTLs14个,位于9个连锁群上;而对于春季萌芽时间共检测到3个QTLs,分别位于3个不同的连锁群上。在检测到的17个QTLs中,每一QTL可解释表型变异的7.6%~15.8%。此外,发现控制叶长、叶宽和叶面积等相关性状的QTLs位于相同的基因组区域,这些QTLs主要位于毛白杨遗传连锁图的TLG2和TLG11以及毛新杨连锁图的TBLG1连锁群上。据控制叶片表型和春季萌芽时间的QTLs所处的基因组区域,可推测叶片表型和春季萌芽时间这两类性状是由各自相应的基因控制。

关键词: 毛新杨×, 毛白杨, 数量性状定位, 叶片表型, 春季萌芽时间

Abstract:

One hundred and twenty progeny of Populus were derived from a cross between female clone “TB01" (Populus tomentosa×P. bolleana) and the male clone “LM50" (P. tomentosa). This population was used to detect quantitative trait loci (QTLs) affecting leaf morphology and spring bud flush. A total of 393 AFLP markers were identified and used to construct a parental specific genetic map using pseudo-test-cross mapping strategy. The total genome length corresponding to 3 265.1 cM for the clone “LM50" map and 1 992 cM for the clone “TB01" map with 4~30 markers per linkage group was obtained. Fourteen QTLs controlling leaf morphology were identified on nine linkage groups, and 3 QTLs affecting spring bud flush were detected on three linkage groups with interval mapping software. The phenotypic variance explained by each QTL ranged from 7.6% (on TBLG14) to 15.8% (on TLG9). Co-localization of QTLs controlling correlated traits such as leafblade length, leafblade width and leafblade area were mainly found on linkage groups TLG2 and TLG11 in the genetic map of clone “LM50", and on linkage group TBLG1 in the genetic map of clone “TB01". Based on the genomic regions of QTLs for leaf morphology and spring bud flush, these two traits seem to be controlled by separate genes.

Key words: Populus tomentosa×, P. bolleana)×, P. tomentosa, QTLs mapping, leaf morphology, spring bud flush