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林业科学 ›› 2004, Vol. 40 ›› Issue (5): 110-115.doi: 10.11707/j.1001-7488.20040518

• 论文及研究报告 • 上一篇    下一篇

部分野生与栽培牡丹种质资源亲缘关系的RAPD研究

孟丽 郑国生   

  1. 山东农业大学生命科学学院,泰安271018北京林业大学园林学院;莱阳农学院生命科学学院,青岛266109
  • 收稿日期:2003-01-13 修回日期:1900-01-01 出版日期:2004-09-25 发布日期:2004-09-25

Phylogenetic Relationship Analysis among Chinese Wild Species and Cultivars of Paeonia sect. Moutan Using RAPD Markers

Meng Li,Zheng Guosheng   

  1. College of Life Science,Shandong Agricultural University Tai'an271018;School of Life Science,Laiyang Agricultural College Qingdao266109
  • Received:2003-01-13 Revised:1900-01-01 Online:2004-09-25 Published:2004-09-25

摘要:

利用RAPD技术对芍药属牡丹组的11个野生牡丹类群和12个栽培牡丹品种间的亲缘关系进行了研究。筛选出的17个单引物和1个组合引物共扩增出191条谱带,其中153条表现多态性(占80.1% ) ,揭示了牡丹组植物丰富的遗传多样性。对扩增结果进行UPGMA聚类分析表明:革质花盘亚组的野生类群与栽培品种聚为一类,革质花盘亚组各野生种与栽培品种间的亲缘关系都比较近。5个野生种与受试牡丹品种间的平均遗传距离值为0.48~0.57,与栽培牡丹间亲缘关系从近到远依次为:杨山牡丹、四川牡丹、卵叶牡丹、紫斑牡丹和矮牡丹。另外,本次RAPD试验扩增出的2 5个特异标记可用于牡丹组内种间、品种间的分类与鉴定。

关键词: 牡丹, 亲缘关系, RAPD

Abstract:

RAPD markers was used to study the phylogenetic relationship among Chinese wild species and cultivars of Paeonia sect. Moutan. 18 primers selected from 210 decamer primers with clear and reproducible patterns generated 153 polymorphic bands(80.1%) among a total of 191 that revealed abundant genetic diversity among sect. Moutan. The number of the RAPD bands was recorded for every accession and 25 special makers could promote classification and identification of sect. Moutan. The cluster analysis based on the RAPD results was performed by UPGMA. The 23 OTUs (Operational taxonomic units) were divided into 3 groups. 5 wild species ( P. rockii, P. jishanensis, P. ostii, P. decomposita, P. qiui) and 11 cultivars got into one group. The genetic distances between every wild species and cultivars were also calculated, and the sequence based on genetic distance (0.48~0.57) from big to small was as follows: P. ostii, P. decomposita, P. qiui, P. rockii and P. jishanensis.

Key words: Paeonia sect. Moutan, Phylogenetic relationship, RAPD