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林业科学 ›› 2003, Vol. 39 ›› Issue (3): 48-52.doi: 10.11707/j.1001-7488.20030308

• 论文及研究报告 • 上一篇    下一篇

运用AFLP技术估计毛白杨及其杂种毛新杨的遗传杂合水平

张德强1 张志毅2 宋婉4 杨凯3   

  1. 北京林业大学毛白杨研究所,北京100083;中国农业科学院作物品种资源研究所,北京100081
  • 收稿日期:2002-09-29 修回日期:1900-01-01 出版日期:2003-05-25 发布日期:2003-05-25

ESTIMATION OF THE GENETIC HETEROZYGOSITY OF POPULUS TOMENTOSA AND ITS HYBRID P.TOMENTOSA×P. BOLLEANA USING AFLP TECHNIQUE

Zhang Deqiang1,Zhang Zhiyi2,Song Wan4,Yang Kai3   

  1. Institute of Populus tomentosa, BFU Beijing 100083;Institute of Crop Germplasm, CAA Beijing 100081
  • Received:2002-09-29 Revised:1900-01-01 Online:2003-05-25 Published:2003-05-25

摘要:

首次报道以毛新杨(Populus tomentosa×P.bolleana)无性系TB01×毛白杨(P.tomentosa)无性系LM50的回交子代120株随机个体为材料,利用扩增性片断长度多态性(AFLPs)标记技术研究毛白杨及其杂种毛新杨的遗传平均杂合水平。研究结果表明:30对不同的AFLP引物组合能够检测到毛白杨的遗传平均杂合水平范围为9.10%~30.19% ,平均为18.71% ;而毛新杨的遗传平均杂合范围为1.39%~20.31% ,平均杂和水平约为8.47%。因此,对于分离群体亲本杂合度来说,毛白杨平均杂合度为毛新杨的2.21倍。

关键词: 毛白杨, 毛新杨, 遗传杂合水平, AFLP

Abstract:

The average heterozygosity of male clone “LM 50” (Populus tomentosa) and its hybrid female clone “TB01”(P.tomentosa×P.bolleana) was estimated, using AFLP (Amplified Fragment Length Polymorphisms) technique, based on random samples with 120 progeny. The hybrids were derived from an interspecific backcross between the female hybrid clone “TB01” and the male clone “LM50”. The result showed that the average heterozygosity was 18.71% with a range of 9.10% ~ 30.19% for male clone “LM50” (P. tomentosa), and 8.47% varied from 1.39% to 20.31% for female clone “TB01” (P.tomentosa×P.bolleana), respectively, using 30 different AFLP primer combinations. Therefore, the average heterozygosity of clone “LM50” was 2.21 times larger than the one of clone “TB01”, based on the segregation population.

Key words: Populus tomentosa, Populus tomentosa×, P. bolleana, Genetic heterozygosity, AFLP technique