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林业科学 ›› 2000, Vol. 36 ›› Issue (zk): 116-119.doi: 10.11707/j.1001-7488.2000S117

• 研究简报 • 上一篇    下一篇

CpTI基因毛白杨的获得

郝贵霞 朱祯 朱之悌   

  1. 中国科学院遗传研究所,北京100101;北京林业大学,北京100083
  • 收稿日期:1998-11-09 修回日期:1900-01-01 出版日期:2001-01-25 发布日期:2001-01-25

OBTAINING OF COWPEA PROTEINASE INHIBITOR TRANSGENIC POPULUS TOMENTOSA

Hao Guixia,Zhu Zhen,Zhu Zhiti   

  1. Institute of Genetics,Chinese Academy of Sciences Beijing 100101;Beijing Forestry University Beijing 100083
  • Received:1998-11-09 Revised:1900-01-01 Online:2001-01-25 Published:2001-01-25

关键词: 毛白杨, 根农杆菌介导法, 豇豆胰蛋白酶抑制剂(CpTI)基因, 转基因植株

Abstract:

In this study,cowpea trypsin inhibitor (CpTI) gene, an insecticidal gene,was introduced into two Populus tomentosa clones by gene transformation mediated by Agrobacterium tumefaciens. The transformed regeneration shoots were obtained directly by leaf discs.In order to established selection condition,leaf discs were cultured in the medium with increasing concentration kanamycin.Kanamycin resistant(kmr)plantlets were obtained by 3~4 cycles screening in selective condition.Then transformed shoots were rooted in the medium containing kanamycin 50mg/L and transferred to greenhouse.The presence of CpTI gene in transgenic plants were confirmed by PCR and PCR-Southern blot.

Key words: P.tomentosa, Agrobacterium tumefaciens, Cowpea trypsin inhibitor gene (CpTI), Transgenic plants