Table 1

Primers used in this study"

Fig.1

Sequence analysis of CfPmt2"

Fig.2

Construction of the CfPMT2 gene knockout vector and acquisition of mutants and complemented strains A. Schematic diagram of the CfPMT2 gene knockout strategy; B. Electrophoretic verification of mutants and complemented strains; M: 5 000 bp DNA marker; ?: H2O negative control; +: WT positive control; Δ: Mutants (No. 29, 46); CS: Complemented strains (No. 17, 33); C. Analysis of CfPMT2 gene expression levels in various strains; **: Indicates extremely significant difference (P<0.01)."

Fig.3

Analysis of vegetative growth, conidiation, and appressorium formation of ΔCfpmt2 A: Vegetative growth of WT, ΔCfpmt2, and ΔCfpmt2/PMT2 on PDA, CM, and MM plates; B: Statistical analysis of colony size of various strains; C: Conidia and appressoria of various strains, and arrows in white color indicate globular balloon-like structures; Scale=10 μm; D: Statistical analysis of conidiation; E: Statistical analysis of germination rate; F: Statistical analysis of appressorium formation rate; G: Statistical analysis of appressorium malformation rate; **: Indicates extremely significant difference (P<0.01)."

Fig.4

Analysis of conidial adhesion of ΔCfpmt2 A. The states of the culture broth of various strains cultured in PDB liquid medium for 3，5，and 7 days; B. Conidia on hydrophobic glass slides before and after sterile water washing; Upper: Unwashed; Lower: After washing; Scale = 20 μm; C. Statistical analysis of conidial adhesion rate of various strains; D. Conidia stained with crystal violet after PBS washing; Scale = 20 μm; E. Statistical analysis of the number of conidia stained with crystal violet by randomly selecting 3?5 fields of view; F. Statistical analysis of OD values of conidia stained with crystal violet of various strains detected by microplate reader; **: Indicates extremely significant difference (P<0.01)."

Fig.5

Analysis of appressorial adhesion of ΔCfpmt2 A. Appressoria on hydrophobic glass slides before and after sterile water washing; Upper: Unwashed; Lower: After washing; Scale = 20 μm; B. Statistical analysis of appressorial adhesion rate of various strains; C. Appressoria stained with crystal violet after PBS washing; Scale = 20 μm; D. Statistical analysis of the number of appressoria stained with crystal violet by randomly selecting 3?5 fields of view; E. Statistical analysis of OD values of appressoria stained with crystal violet of various strains detected by microplate reader; **: Indicates extremely significant difference (P<0.01)."

Fig.6

qRT-PCR analysis of the relative expression levels of GAA1, GPI8 and MSB2 genes in the ΔCfpmt2"

Fig.7

Sensitivity assay of ΔCfpmt2 under cell wall stress and oxidative stress A. Growth of WT， ΔCfpmt2， and ΔCfpmt2/PMT2 on PDA plates supplemented with 400 μg·mL−1 Congo Red (CR); B. Growth of WT， ΔCfpmt2， and ΔCfpmt2/PMT2 on PDA plates supplemented with 5 mmol·L−1 H2O2 and 10 mmol·L−1 H2O2; C. Statistical analysis of inhibition rate of various strains; **: Indicates extremely significant difference (P<0.01)."

Fig.8

Analysis of mycelium formation and development of ΔCfpmt2 A. Mycelium formation of various strains in shake flask cultures; B. Analysis of CFW staining of mycelia from various strains, and arrows in white color indicate septa; C. Analysis of DHR 123 staining of mycelia from various strains."

Fig.9

Analysis of the distribution of conidia, appressorial chitin and ROS in ΔCfpmt2 A. CFW staining of conidial suspensions from different strains cultured on hydrophobic glass slides for 2，4，6 and 24 h; B. DHR 123 staining of conidial suspensions from different strains cultured on hydrophobic glass slides for 2，4，6 and 24 h."

Fig.10

Pathogenicity assay of ΔCfpmt2 A. Pathogenicity of different strains inoculated on intact Camellia oleifera leaves; B. Statistical analysis of lesion size on intact camellia oleifera leaves; C. Pathogenicity of different strains inoculated on wounded C. oleifera leaves; D. Statistical analysis of lesion size on wounded C. oleifera leaves; **: Indicates extremely significant difference （P<0.01）; *: Indicates significant difference (P<0.05)."