Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (3): 48-58.doi: 10.11707/j.1001-7488.20220306
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Zhiyang Li,Xiaolin Chen,Lili Li,Shiping Xu,Yuanhao He*
Received:
2021-01-15
Online:
2022-03-25
Published:
2022-06-02
Contact:
Yuanhao He
CLC Number:
Zhiyang Li,Xiaolin Chen,Lili Li,Shiping Xu,Yuanhao He. Transcriptome Analysis of Camellia olefolia Root and the Endophytic Bacteria Bacillus Subtilis at the Early Stage of Their Interaction[J]. Scientia Silvae Sinicae, 2022, 58(3): 48-58.
Table 1
Primers used for qRT-PCR gene expression analysis"
基因名称 Gene name | 引物 Primer(5′-3′) | 扩增长度 Amplification length/bp | 退火温度 Annealing temperature(Tm)/℃ |
POD | TCATACATTCGGACGGGCTG AGTTGTAAAGGCGTGGGGTC | 104 | 56.9 |
RBOHC | ATCGCTCAACCATGCCAAGA ATCGCTCAACCATGCCAAGA | 109 | 57.0 |
TCH4 | AACTCAAGCTTGTGCCTGGA TCATGTGTTGGCCCTTGTGA | 84 | 56.8 |
TIFY | TTCCCGGCTGATAAGGCAAA TTTTGGACCGGTGTTGAAGC | 105 | 56.3 |
XTH | GGGGAATTTGAGTGGTGATCCT TGGAAATCAGCAGTCGGGTC | 104 | 57.0 |
EF1α | CAAAGAAGGGTGCCAAGTGA ACCAAACAACCGACCTACGA | 128 | 56.2 |
Table 2
Summary of De novo data generated for C. oleifera transcriptomes inoculated with endophytic bacteria at different time points"
时间 Time/hpi | 总序列数 Total reads | 唯一比对读段 Number of uniquely mapped reads | 比对率 Proportion of mapped reads (%) | GC含量 GC percentage(%) | Q30含量 Q30 percentage(%) |
0 | 139 674 846 | 80 575 830 | 69.58 | 43.69 | 92.2 |
6 | 130 079 280 | 78 164 416 | 72.83 | 43.89 | 92.19 |
12 | 124 726 484 | 72 206 074 | 70.34 | 44.21 | 92.18 |
24 | 135 478 312 | 79 027 352 | 70.85 | 43.98 | 92.29 |
N50长度 N50 length/bp | 1 175 866 | ||||
平均长度 Average length/bp | 1 175 866 | ||||
比对率 Unique mapped ratio (%) | 71.13 | ||||
注释的基因数量 Annotated gene number | 67 700 |
Fig.4
Heat map diagram of the expression levels of DEGs A: Heat map diagram of the expression levels of DEGs which are involved in plant hormone signal transduction. B: Heat map diagram of the expression levels of DEGs which are involved in plant-pathogen interaction. C: Heat map diagram of theexpression levels of DEGs which are involved in plant phenylpropanoid biosynthesis."
Table 3
DEGs of plant hormone signal transduction pathway"
家族 Family | 基因ID Gene ID | 表达量变化的log2值 log2 value of fold change | 描述(符号) Description(Symbol) | ||
h6 | h12 | h24 | |||
生长素响应基因家族SAUR The auxin response gene family SAUR | Unigene0224095 | 5.271 017 | -1.797 32 | 0.397 943 | 生长素诱导蛋白 Auxin-induced protein X15(SAUR15) |
Unigene0208543 | -2.391 3 | 0.664 469 | -0.731 89 | 生长素诱导蛋白 Auxin-induced protein 15A(SAUR32) | |
Unigene0224842 | -4.887 15 | 5.342 368 | -3.498 13 | 生长素诱导蛋白 Auxin-induced protein 15A (SAUR50) | |
糖苷水解酶家族GH16 Glycoside hydrolase family GH16 | Unigene0074914 | 4.234 786 | -0.121 78 | -0.231 35 | 木葡聚糖内糖基转移酶/水解蛋白酶 Xyloglucan endotransglucosylase/hydrolase protein 23(XTH25) |
Unigene0115871 | 3.840 573 | -0.004 68 | -0.151 34 | 木葡聚糖内糖基转移酶/水解蛋白酶 Xyloglucan endotransglucosylase/hydrolase protein 22(XTH22) | |
TIFY家族基因 TIFY family gene | Unigene0041523 | 4.115 512 | -1.814 64 | -0.207 01 | 茉莉酮酸酯ZIM结构域蛋白 Jasmonate ZIM domain-containing protein (TIFY9) |
Unigene0098739 | 3.869 918 | -1.349 38 | 0.522 57 | 茉莉酮酸酯ZIM结构域蛋白 Jasmonate-zim-domain protein (TIFY10B) | |
生长素早期响应基因家族AUX/IAA Auxin early response gene family AUX/IAA | Unigene0082739 | 2.984 088 | -2.344 58 | -0.231 92 | 生长素 Auxin-responsive protein IAA(IAA17) |
转录因子bZIP Transcription factor bZIP | Unigene0061614 | -2.504 95 | 1.864 647 | -0.385 94 | 脱落酸应答元件结合因子 ABA responsive element binding factor(ABI5) |
Unigene0118601 | -4.000 41 | 1.824 728 | -1.405 45 | 含碱性亮氨酸拉链结构域的蛋白 Basic-leucine zipper domain-containing protein(TGAL4) | |
GH3基因家族 GH3 gene family | Unigene0061751 | -3.476 34 | 1.027 397 | 1.369 855 | 生长素响应GH3基因家族 Auxin responsive GH3 gene family (GH3) |
DELLA基因家族 DELLA gene family | Unigene0089563 | -7.258 48 | 5.469 323 | -10.347 1 | DELLA蛋白 DELLA protein (GAI) |
Table 4
DEGs of plant-pathogen interaction pathway"
家族 Family | 基因ID Gene ID | 表达量变化的log2值 log2 value of fold change | 描述(符号) Description(Symbol) | ||
h6 | h12 | h24 | |||
CAM | Unigene0154452 | 6.431 758 | 0.194 622 | -1.359 71 | 钙调蛋白 Calmodulin (CAM2) |
MYB | Unigene0006849 | 5.059 191 | -3.267 78 | 2.442 811 | MYB相关蛋白Myb4 Myb-related protein Myb4 (MYB102) |
Unigene0091260 | 3.342 609 | -1.393 12 | 1.400 094 | 转录因子MYB36 Transcription factor MYB36 (MYB36) | |
Unigene0079478 | 2.615 85 | -2.153 92 | 1.069 583 | 转录因子MYB Transcription factor MYB (MYB4) | |
RPM | Unigene0140632 | -3.087 74 | 1.679 16 | 1.575 752 | 抗病蛋白 Disease resistance protein (RPM1) |
Unigene0076986 | -2.419 19 | 1.434 07 | -0.188 74 | 抗病蛋白 Disease resistance protein At4g27220 (RPS5) | |
Unigene0134868 | -3.151 73 | 0.296 655 | 1.008 148 | 核苷酸结合位点-富含亮氨酸重复蛋白 Nucleotide-binding site leucine-rich repeat protein (At4g27190) | |
RBOH | Unigene0041782 | 2.166 077 | 0.104 336 | -1.046 16 | 呼吸爆发氧化酶 Respiratory burst oxidase (RBOHC) |
MAPK | Unigene0176521 | 1.873 724 | -0.689 07 | 0.553 341 | MAPK激酶3 Mitogen-activated protein kinase 3 (NTF4) |
Unigene0008839 | -1.662 43 | 0.789 679 | -0.486 96 | MAPK同源物 Mitogen-activated protein kinase homolog MMK2(MMK2) | |
RPM1互作蛋白 RPM1 interacting protein | Unigene0126538 | 1.872 688 | -0.307 55 | -0.702 38 | RPM1互作蛋白 RPM1-interacting protein (RIN4) |
LysM受体类激酶 LysM receptor kinases | Unigene0043775 | -3.029 47 | -0.579 95 | -0.056 76 | LysM受体类激酶 LysM domain receptor-like kinase (CERK1) |
气味结合蛋白 Odorant binding protein | Unigene0003058 | -2.260 62 | 0.780 021 | -1.123 88 | 气味结合蛋白 Protein ODORANT1 (ODO1) |
Table 5
DEGs of phenylpropanoid biosynthesis pathway"
家族 Family | 基因ID Gene ID | 表达量变化的log2值 log2 value of fold change | 描述(符号) Description(Symbol) | ||
h6 | h12 | h24 | |||
过氧化物酶基因家族 Peroxidase gene family(POD) | Unigene0059080 | 3.777 526 | -0.643 98 | 0.573 304 | 类木质素形成阴离子过氧化物酶 Lignin-forming anionic peroxidase-like (PER5) |
Unigene0163356 | 3.407 953 | -1.814 69 | 0.370 634 | 木质素形成阴离子过氧化物酶 Lignin-forming anionic peroxidase (pod) | |
Unigene0163359 | 1.999 275 | -0.126 23 | 0.149 761 | 类过氧化物酶 Peroxidase A2-like(PER58) | |
糖苷水解酶家族 Glycoside hydrolase family(GH) | Unigene0164751 | -1.465 3 | 1.472 489 | -1.055 36 | 类β-葡萄糖苷酶12 Beta-glucosidase 12-like(BGLU12) |
Unigene0001162 | -1.559 93 | 0.931 72 | 0.399 258 | 类β-葡萄糖苷酶11 Beta-glucosidase 11-like(AA7GT) | |
Unigene0055474 | -2.519 19 | 1.392 39 | -0.277 75 | β-葡萄糖苷酶 Beta glucosidase 11(BGLU11) | |
肉桂醇脱氢酶 Cinnamyl alcohol (CAD) dehydrogenase | Unigene0086215 | -1.826 91 | -0.280 1 | 0.181 386 | 肉桂醇脱氢酶1 Cinnamyl alcohol dehydrogenase 1 (CAD1) |
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