油茶抗性无性系茶皂素含量对茶籽象幼虫肠道菌群结构的影响

doi:10.11707/j.1001-7488.20220712

Abstract

Abstract:

Objective: The aims of this study are to determine whether the content of tea saponin among Camellia oleifera resistant clones affect the composition of C. sinensis' gut microbiota, and their survival rates, and further to clarify the role of tea saponin on C. sinensis resistance, so as to provide theoretical support for the selection and breeding of C. sinensis resistant varieties and the development of new green prevention and control techniques against C. chinensis. Method: C. chinensis was inoculated into five C. oleifera clones with different content of tea saponin. After larvae matured, the survival of larvae on different clones was calculated and the correlation between the larvae survival rate and the content of tea saponin was analyzed. Sequence obtained from 16S rRNA region was used to compare and evaluate the effects of C. oleifera clones on taxonomic composition, alpha diversity, beta diversity and marker species of gut microbiota. Result: The correlative regression analysis showed that the correlation between the survival of C. chinensis and the content of tea saponin was y = 0.252 5x² - 10.096x +107.47 (R² = 0.718 9). The survived number of C. chinensis decreased with the increase of tea saponin content. Through the analysis of the gut microbiota structure of C.chinensis, it was found that alpha and beta diversities of C. chinensis gut microbiota in Changlin 166, and 40 clones with higher content of tea saponin were significantly different from those in Changlin 55, 53 and 3 clones (P < 0.05). The result of marker species analysis showed that 51 differential OTUs were significantly enriched in the gut of C. chinensis with higher tea saponin group, residing in the genera of Flavobacterium, Azospirillum, Crolobacter, Pseudomonas and Acintobacter, and the last two genera accounted for the highest proportion. Conclusion: Colones with high content of tea saponin can significantly affect survival of C. chinensis, its gut microbiota diversity, and microbial composition. The tea saponin content can be considered as an important chemical index for the breeding of insect resistant varieties for C. oleifera.

Key words: Curculio chinensis, Camellia oleifera, constitutive resistance, tea saponin content, gut microbiota, marker species

CLC Number:

S718.7

Shouke Zhang,Zikun Li,Hao Yin,Wei Zhang,Jinping Shu,Haojie Wang,Xudong Zhou,Yangdong Wang. Effects of Saponin Content in Camellia oleifera Resistant Clones on the Gut Microbiome Structure of the Seed Pest Curculio chinensis (Coleoptera: Curculionidae)[J]. Scientia Silvae Sinicae, 2022, 58(7): 120-127.

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分组1 Group 1	分组2 Group 2	样本大小 Sample size	排列数 Permutations	R	P
长林40 Changlin 40	长林55 Changlin 55	36	999	0.935	0.001
长林40 Changlin 40	长林3 Changlin 3	36	999	0.992	0.001
长林40 Changlin 40	长林53 Changlin 53	36	999	0.977	0.001
长林40 Changlin 40	长林166 Changlin 166	36	999	0.194	0.004
长林55 Changlin 55	长林3 Changlin 3	36	999	0.329	0.001
长林55 Changlin 55	长林53 Changlin 53	36	999	0.112	0.026
长林55 Changlin 55	长林166 Changlin 166	36	999	0.955	0.001
长林3 Changlin 3	长林53 Changlin 53	36	999	0.079	0.032
长林3 Changlin 3	长林166 Changlin 166	36	999	0.991	0.001
长林53 Changlin 53	长林166 Changlin 166	36	999	0.984	0.001

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Effects of Saponin Content in Camellia oleifera Resistant Clones on the Gut Microbiome Structure of the Seed Pest Curculio chinensis (Coleoptera: Curculionidae)

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