毛竹PheFT1基因的表达、蛋白互作及生物学功能分析

doi:10.11707/j.1001-7488.LYKX20240069

Abstract

Abstract: Objective OsFTL1 in rice is a flowering regulator which can also affect leaf size, plant height and spike structure. At the early stage, 18 FT genes were identified from Phyllostachys edulis (moso bamboo), one of which exhibited the closest genetic relationship with OsFTL1. This study aims to clarify the biological function of this gene in moso bamboo in order to provide a theoretical basis for revealing the mechanism of flowering in moso bamboo. Method Real-time quantitative PCR was used to detect the tissue-specific expression of PheFT1 and its response to photoperiod. The subcellular localization of PheFT1 protein was analyzed by PEG-mediated assay. Agrobacterium-mediated transfer of PheFT1 gene into Arabidopsis thaliana was used to produce overexpression plants and ft mutant complemented plants. The phenotypic differences between overexpression plants and wild-type plants as well the differences between ft mutant complementation plants and ft mutant plants were compared to analyze PheFT1 biological function. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation assays were used to analyze the interacting proteins of PheFT1. Result The results of bioinformatics analysis showed that the CDS of PheFT1 gene was 537 bp in full length, encoding 178 amino acids, belonging to the PEBP protein family. The results of subcellular localization showed that PheFT1 protein was localized in the nucleus and cytoplasm. The real-time quantitative PCR showed that the PheFT1 gene was expressed in root, stem, leaf and lateral bud, with higher expression in lateral bud and stem, and lower expression in leaf. PheFT1 exhibited a strong circadian rhythm under long-day. Ectopic expression showed that the PheFT1 gene induced earlier flowering, thinner stem and shorter plant height in A. thaliana. Moreover, the results of protein interaction showed that PheFT1 was able to interact with PheGF14 and PheFD proteins. Conclusion PheFT1 is a flowering promoting factor in moso bamboo, and also participates in the stem development and height growth. This study provides a reference for further revealing the molecular mechanism of the PheFT1 gene involved in flowering and development in moso bamboo.

Key words: Phyllostachys edulis, FLOWERING LOCUS T gene, PEBP protein family, protein interaction, flowering

CLC Number:

S795.7

Yan Xiaoling, Hao Qin, Shen Zi, Zhang Yujia, Guo Xiaoqin. Expression, Protein Interaction and Biological Function Analysis of PheFT1 Gene in Moso Bamboo[J]. Scientia Silvae Sinicae, 2025, 61(4): 140-152.

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Expression, Protein Interaction and Biological Function Analysis of PheFT1 Gene in Moso Bamboo

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