牡丹秋季开花过程中生理生化变化及DNA甲基化差异

doi:10.11707/j.1001-7488.20210506

Abstract

Abstract:

Objective: Tree peony (Paeonia suffruticosa) has a short and concentrated florescence, in order to explore the mechanism of autumn flowering, we studied the effects of gibberellin and DNA methylation during autumn flowering of tree peony. Method: Before natural defoliation in the middle September, all plants of the variety (P. suffruticosa 'Togawakan', a spring and autumn flowering variety of tree peony planted in the open field in Luoyang, Henan Province) were divided into 4 treatment groups: defoliation, defoliation + 650 mg·L^-1 gibberellin (GA₃), defoliation + 500 mg·L^-1 paclobutrazol (PP₃₃₃) processing, and no treatment. Growth performance of the treatment groups and the control was observed and recorded during autumn bud germination and flowering process, and the physiological and biochemical processes and methylation of genomic DNA of the treatment groups were measured. Result: 1) All of the four treatment groups could naturally flower in the autumn, but applying the treatments could promote 'Togawakan' autumn secondary flowering in advance and increase the effect of the flowering uniformity. Particularly, the treatment group of defoliation + 650 mg·L^-1 GA₃ had the best effect on promoting 'Togawakan' to sprout and flower, with a sprouting rate of 81.4%, 11 days after the treatment, and initial flowering started 42 days after treatment, 30 days earlier than the control group. 2) The defoliation + 650 mg·L^-1 GA₃, defoliation and defoliation + 500 mg·L^-1 PP₃₃₃ all contributed to fast and neat sprout and growth of the variety 'Togawakan', and the acceleration of flowering decreased in turn, suggesting that defoliation could promote sprout and growth of the buds, applying GA₃ has superposition promoting effect, PP₃₃₃ could offset the promoting effect of defoliation to a certain degree. Defoliation exerted an inhibitory effect on GA₃ and GA₄ content for a short time at first, then followed by an increase of GA₃ and GA₄ contents, and a decrease of ABA content in buds. The application of GA₃ enhanced the intensity of increased GA₃ and GA₄ contents and decreased ABA content, and prolonged the rising period of GA₃ and GA₄ contents, while the application of PP₃₃₃ showed the opposite effect. 3) Sprouting and flowering were accompanied by the consumption of a large amount of starch and soluble sugars, and a high concentration of soluble sugar may be beneficial to flowering. 4) MSAP test showed that the variation range of total DNA methylation in 'Togawakan' during the whole experiment period was 57.3%-72.4%, reaching the highest level of total DNA methylation before sprouting, and followed by a gradual decrease. The methylation of the control 1 day after treatments, as the baseline was compared with those of the treatment groups in different treatment periods. It was found that the methylation in treatment groups was more actively changing, and the changes were concentrated in two models, demethylation and hypermethylation. The demethylation rate of each treatment group increased with time and was higher than that of the control group in the same period. It was speculated that the high demethylation rate may be more conducive to the bursting of 'Togawakan' in autumn. Conclusion: P. suffruticosa 'Togawakan' could naturally sprout and flower in autumn, defoliation promoted endogenous GA₃ synthesis, combined with the superposition effect of exogenous GA₃, increased GA₃ and inhibited ABA in vivo, promoted its secondary flowering in autumn, and the flowering period was neat. Exogenous PP₃₃₃ application to some extents counteract the synthesis of endogenous GA₃ caused by defoliation. Sprouting to flowering was accompanied by a large amount of sugar consumption, high concentration of soluble sugar might be conducive to bud sprouting. 'Togawakan' can self-regulate the methylation status at locus CG of relevant DNA sequences, thus inducing flower buds to sprout in autumn, and application of the treatments could trigger this regulation process ahead of time, in which high DNA demethylation rate was more likely to promote autumn germination and flowering.

Key words: Paeonia suffruticosa, autumn flowering, defoliation, gibberellin, paclobutrazol, DNA methylation

CLC Number:

S718.43

Xue Yuan,Tao Yuan,Shaodan Liu. Variation in Physiological and Biochemical Properties and DNA Methylation Patterns during Autumn Flowering of Tree Peony(Paeonia Suffruticosa)[J]. Scientia Silvae Sinicae, 2021, 57(5): 53-67.

Figures/Tables 16

Fig.1

Table 1

Fig.2

Table 2

Table 3

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

Fig.8

Table 4

Table 5

Table 6

Table 7

Table 8

References 0

	陈新露. 2000. 中国秋发牡丹品种资源及秋发机理研究. 北京: 北京林业大学博士学位论文.
	Chen X L. 2000. Studies on germplasm of autumn-flowering tree peonies and its autumn-flowering mechanism in China. Beijing: PhD thesis of Beijing Forestry University. [in Chinese]
	迟东明, 果朋忠, 宋伟, 等. 赤霉素对牡丹促成栽培生长发育的影响. 安徽农业科学, 2007, 35 (22): 6757, 6763.
	Chi D M , Guo P Z , Song W , et al. Effect of gibberellin on growth and development of tree peony in forcing culture. Journal of Anhui Agricultural Sciences, 2007, 35 (22): 6757, 6763.
	盖树鹏, 张风, 张玉喜, 等. 低温解除牡丹休眠进程中基因组DNA甲基化敏感扩增多态性(MSAP)分析. 农业生物技术学报, 2012, 20 (3): 261- 267. doi: 10.3969/j.issn.1674-7968.2012.03.005
	Gai S P , Zhang F , Zhang Y X , et al. Analysis of genomic DNA methylation during chilling induced endo-dormancy release by methylation sensitive amplified polymorphism(MSAP) technology in tree peony (Paeonia suffruticosa). Journal of Agricultural Biotechnology, 2012, 20 (3): 261- 267. doi: 10.3969/j.issn.1674-7968.2012.03.005
	高志民, 王雁, 李振坚, 等. 牡丹开花前后营养变化分析研究. 林业科学研究, 2007, 20 (3): 390- 393. doi: 10.3321/j.issn:1001-1498.2007.03.017
	Gao Z M , Wang Y , Li Z J , et al. Study on the changes of nutrients and mineral elements in tree peony before and after flowering. Forest Research, 2007, 20 (3): 390- 393. doi: 10.3321/j.issn:1001-1498.2007.03.017
	黄睿. 2007. 多效唑对盆栽牡丹生理生化特性的影响研究. 长沙: 湖南农业大学硕士学位论文.
	Huang R. 2007. Study on effects of the physiological and biochemical characteristics of PP₃₃₃ on potted peony. Changsha: MS thesis of Hunan Agricultural University. [in Chinese]
	蒋政, 孙李勇, 刘旭, 等. 常春二乔玉兰春夏季开花节律及营养效应研究. 植物研究, 2019, 39 (2): 192- 199.
	Jiang Z , Sun L Y , Liu X , et al. Nutritional effect and rhythm of spring and summer flowering in Magnolia soulangeana 'Changchun'. Bulletin of Botanical Research, 2019, 39 (2): 192- 199.
	李波, 夏秀英, 刘思. 蓝莓花芽休眠与解除过程中生理生化变化及DNA甲基化差异分析. 植物生理学报, 2015, 51 (7): 1133- 1141.
	Li B , Xia X Y , Liu S . Changes in physiological and biochemical properties and variation in DNA methylation patterns during dormancy and dormancy release in blueberry (Vaccinium corymbosum L). Plant Physiology Journal, 2015, 51 (7): 1133- 1141.
	李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
	Li H S . Principles and techniques of plant physiological biochemical experiment. Beijing: Higher Education Press, 2000.
	刘嘉仪. 2017. '雪球'海棠二次开花试验研究. 长春: 吉林农业大学硕士学位论文.
	Liu J Y. 2017. Study on the secondary blooming of Malus 'Snowdrift'. Changchun: MS thesis of Jilin Agricultural University. [in Chinese]
	吕双庆, 李生秀. 多效唑对旱地小麦一些生理、生育特性及产量的影响. 植物营养与肥料学报, 2005, 11 (1): 92- 98. doi: 10.3321/j.issn:1008-505X.2005.01.015
	Lü S Q , Li S X . Effects of PP₃₃₃ spraying on some physiological, morphological characteristics and yield of wheat on dryland with different plant density. Plant Nutrition and Fertilizer Science, 2005, 11 (1): 92- 98. doi: 10.3321/j.issn:1008-505X.2005.01.015
	潘瑞炽. 植物生理学. 北京: 高等教育出版社, 2012.
	Pan R C . Plant physiology. Beijing: Higher Education Press, 2012.
	曲波, 张微, 陈旭辉, 等. 植物花芽分化研究进展. 中国农学通报, 2010, 26 (24): 109- 114.
	Qu B , Zhang W , Chen X H , et al. Research progress of flower bud differentiation mechanism of plant. Chinese Agricultural Science Bulletin, 2010, 26 (24): 109- 114.
	全璨璨. 2009. "国庆牡丹"露地栽培二次开花技术研究. 北京: 北京林业大学硕士学位论文.
	Quan C C. 2009. Studies on reflowering of "National-flowering Tree Peonia" on open field. Beijing: MS thesis of Beijing Forestry University. [in Chinese]
	任小林, 李海峰, 弓德强, 等. 秋施乙烯利和赤霉素对牡丹萌芽及开花的影响. 西北植物学报, 2004, 24 (5): 895- 898. doi: 10.3321/j.issn:1000-4025.2004.05.027
	Ren X L , Li H F , Gong D Q , et al. Effects of application of ethephon and GA₃ in fall on bud sprouting and flowering of peony. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24 (5): 895- 898. doi: 10.3321/j.issn:1000-4025.2004.05.027
	宋海凤, 李绍才, 孙海龙, 等. 根施不同浓度多效唑对紫穗槐生长特性和相关生理指标的影响. 植物生理学报, 2015, 51 (9): 1495- 1501.
	Song H F , Li S C , Sun H L , et al. Effects of soil-applied paclobutrazol on growth and physiological characteristics of Amorpha fruticosa. Plant Physiological Journal, 2015, 51 (9): 1495- 1501.
	孙晓萍, 樊丽娟, 陈亮. 杭州市紫薇花期调控成果初报. 中国园林, 2016, 32 (12): 32- 37. doi: 10.3969/j.issn.1000-6664.2016.12.007
	Sun X P , Fan L J , Chen L . Primary report on the production of crape-myrtle flowering regulation in Hangzhou. Chinese Landscape Architecture, 2016, 32 (12): 32- 37. doi: 10.3969/j.issn.1000-6664.2016.12.007
	王荣. 2007. 牡丹花芽分化及二次开花特性的研究. 北京: 北京林业大学博士学位论文.
	Wang R. 2007. Researches on bud differentiation and re-blooming characteristic of tree peony. Beijing: PhD thesis of Beijing Forestry University. [in Chinese]
	王子成, 聂丽娟, 何艳霞. 离体条件下5-氮杂胞嘧啶核苷对菊花DNA甲基化和表型性状的影响. 园艺学报, 2009, 36 (12): 1783- 1790. doi: 10.3321/j.issn:0513-353X.2009.12.010
	Wang Z C , Nie L J , He Y X . The effect of 5-azacytidine to the DNA methylation and morphogenesis character of Chrysanthemum during in vitro growth. Acta Horticulturae Sinica, 2009, 36 (12): 1783- 1790. doi: 10.3321/j.issn:0513-353X.2009.12.010
	温璐华, 王真, 庄维兵, 等. 外源GA₄处理解除果梅花芽休眠的生理效应研究. 中国南方果树, 2015, 44 (5): 16- 22.
	Wen L H , Wang Z , Zhuang W B , et al. Physiological effect of exogenous GA₄ on dormancy release of Japanese apricot flower buds. South China Fruits, 2015, 44 (5): 16- 22.
	张华, 聂艳, 王定跃, 等. 乙烯利和多效唑对簕杜鹃生长开花及生理特性的影响. 林业科学, 2018, 54 (10): 46- 55. doi: 10.11707/j.1001-7488.20181006
	Zhang H , Nie Y , W D Y , et al. Effects of ETH and PP₃₃₃ on the growth, florescence and physiological properties of Bougainvillea spectabilis. Scientia Silvae Sinicae, 2018, 54 (10): 46- 55. doi: 10.11707/j.1001-7488.20181006
	张涛, 司福会, 张玉喜, 等. 外源GA₃影响牡丹花芽DNA甲基化水平和相关酶基因的表达分析. 中国农业科学, 2018, 51 (18): 3561- 3569. doi: 10.3864/j.issn.0578-1752.2018.18.012
	Zhang T , Si F H , Zhang Y X , et al. Effect of exogenous gibberellin on DNA methylation level and expression of related enzyme genes in tree peony floral buds. Scientia Agricultura Sinica, 2018, 51 (18): 3561- 3569. doi: 10.3864/j.issn.0578-1752.2018.18.012
	张文娟. 2005. 冷藏和赤霉素(GA₃)处理对牡丹促成栽培影响的研究. 北京: 北京林业大学硕士学位论文.
	Zhang W J. 2005. Study on the effects of chilling and GA₃ treatments on forcing culture of tree peony. Beijing: MS thesis of Beijing Forestry University. [in Chinese]
	张秀新. 2004. 秋发牡丹露地二次开花调控栽培及其开花生理的研究. 北京: 北京林业大学博士学位论文.
	Zhang X X. 2004. Studies on forcing culture of autumn-flowering tree-peonies in field and its reflowering physiology. Beijing: PhD thesis of Beijing Forestry University. [in Chinese]
	郑国生, 盖树鹏, 盖伟玲. 低温解除牡丹芽休眠进程中内源激素的变化. 林业科学, 2009, 45 (2): 48- 52. doi: 10.3321/j.issn:1001-7488.2009.02.009
	Zheng G S , Gai S P , Gai W L . Changes of endogenous hormones during dormancy release by chilling in tree peony. Scientia Silvae Sinicae, 2009, 45 (2): 48- 52. doi: 10.3321/j.issn:1001-7488.2009.02.009
	周华. 2015. 基于转录组比较的牡丹开花时间基因发掘. 北京: 北京林业大学博士学位论文.
	Zhou H. 2015. Discovery of genes associated with flowering time in tree peonies based on transcriptome comparison. Beijing: PhD thesis of Beijing Forestry University. [in Chinese]
	Chandler J , Dean C . Factors influencing the vernalization response and flowering time of late flowering mutants of Arabidopsis thaliana (L) Heynh. Journal of Experimental Botany, 1994, 45 (9): 1279- 1288. doi: 10.1093/jxb/45.9.1279
	Finnegan E J , Genger R K , Kovac K , et al. DNA methylation and the promotion of flowering by vernalization. Proceedings of the National Academy of Sciences of the USA, 1998, 95 (10): 5824- 5829. doi: 10.1073/pnas.95.10.5824
	Hedden P , Phillips A L . Gibberellin metabolism: new insights revealed by the genes. Trends in Plant Science, 2000, 5 (12): 523- 530. doi: 10.1016/S1360-1385(00)01790-8
	Li M H , Hoch G , Korner C . Source/sink removal affects mobile carbohydrates in Pinus cembra at the Swiss treeline. Trees-Structure&Function, 2002, 16 (4/5): 331- 337.
	Lízal P , Relichová J . The effect of day length, vernalization and DNA demethylation on the flowering time in Arabidopsis thaliana. Physiologia Plantarum, 2001, 113 (1): 121- 127. doi: 10.1034/j.1399-3054.2001.1130116.x
	Lukens L N , Zhan S H . The plant genome's methylation status and response to stress, implications for plant improvement. Current Opinion in Plant Biology, 2007, 10 (3): 317- 322. doi: 10.1016/j.pbi.2007.04.012
	Or E. 2009. Grape bud dormancy release the molecular aspect//Roubelakis-Angelakis K A. Grapevine molecular physiology & biotechnology. 2^nd ed. Springer Science, Germany, 1-29.
	Pharis R P , King R W . Gibberellins and reproductive development in seed plants. Annual Review of Plant Physiology, 1985, 36, 517- 568. doi: 10.1146/annurev.pp.36.060185.002505
	Raessler M , Wissuwa B , Breul A , et al. Chromatographic analysis of major non-structural carbohydrates in several wood species-an analytical approach for higher accuracy of data. Anal Methods-UK, 2010, 2 (5): 532- 538. doi: 10.1039/b9ay00193j
	Richards E J . DNA methylation and plant development. Trends in Genetics, 1997, 13 (8): 319- 323. doi: 10.1016/S0168-9525(97)01199-2
	Rinne P L H , Willing A , Vahala J , et al. Chilling of dormant buds hyperinduces FLOWERING LOCUS T and recruits GA-inducible 1, 3-beta-glucanases to reopen signal conduits and release dormancy in Populus. The Plant Cell, 2011, 23 (1): 130- 146. doi: 10.1105/tpc.110.081307
	Xue J , Li T , Wang S , et al. Elucidation of the mechanism of reflowering in tree peony (Paeonia suffruticosa) 'Zi Luo Lan' by defoliation and gibberellic acid application. Plant Physiology and Biochemistry, 2018, 132, 571- 578. doi: 10.1016/j.plaphy.2018.10.004
	Xue J , Li T , Wang S , et al. Defoliation and gibberellin synergistically induce tree peony flowering with non-structural carbohydrates as intermedia. Journal of Plant Physiology, 2019, 233, 31- 41. doi: 10.1016/j.jplph.2018.12.004
	Zhao J , Li G , Yi G X , et al. Comparison between conventional indirect competitive enzyme-linked immunosorbent assay(icELISA) and simplified icELISA for small molecules. Analytica Chimica Acta, 2006, 571 (1): 79- 85. doi: 10.1016/j.aca.2006.04.060

处理 Treatment	日期Date(天数)
处理 Treatment	09 -16 (0 d)	09-17 (1 d)	09-19 (3 d)	09-20 (4 d)	09-21 (5 d)	09-22 (6 d)	09-27 (11 d)	10-03 (17 d)	10-10 (24 d)	10-18 (32 d)	10-28 (42 d)
对照 Control	—	√	√	—	—	√	√	√	√	√	√
处理A Treatment A	脱叶 Defoliation	√	√	—	—	√	√	√	√	√	√
处理B Treatment B	脱叶 Defoliation	√	GA₃ √	GA₃	GA₃	√	√	√	√	√	√
处理C Treatment C	脱叶 Defoliation	√	PP₃₃₃ √	PP₃₃₃	PP₃₃₃	√	√	√	√	√	√

引物类型Primer type	引物名称Primer name	引物序列Primer sequence(5′—3′)
接头Adapter	EcoRⅠ-adaptor top	CTCGTAGACTGCGTACC
	EcoRⅠ-adaptor bottom	AATTGGTACGCAGTCTAC
	HpaⅡ-MspⅠadaptor top	GATCTGAGTCCTGCT
	HpaⅡ-MspⅠadaptor bottom	CGAGCAGGACTCATGA
预扩增引物 Pre-amplification primers	EcoRⅠ-0	GACTGCGTACCAATT
预扩增引物 Pre-amplification primers	HpaⅡ-MspⅠ-0	ATCATGAGTCCTGCTCGG

组合名称 Combination name	引物组合 Primer combination
MSAP18	EcoRⅠ-AT+HpaⅡ-MspⅠ-TTC
MSAP21	EcoRⅠ-AC+HpaⅡ-MspⅠ-AAT
MSAP22	EcoRⅠ-AC+HpaⅡ-MspⅠ-AAC
MSAP23	EcoRⅠ-AC+HpaⅡ-MspⅠ-AAG
MSAP25	EcoRⅠ-AC+HpaⅡ-MspⅠ-ACG
MSAP26	EcoRⅠ-TA+HpaⅡ-MspⅠ-AAT
MSAP27	EcoRⅠ-TA+HpaⅡ-MspⅠ-AAC
MSAP28	EcoRⅠ-TA+HpaⅡ-MspⅠ-AAG
MSAP29	EcoRⅠ-TA+HpaⅡ-MspⅠ-ACC
MSAP30	EcoRⅠ-TA+HpaⅡ-MspⅠ-ACG

采样日期 Sampling date	对照 Control	处理A Treatment A	处理B Treatment B	处理C Treatment C
半甲基化位点数(比例) Hemi-methylation loci(rate)
09-17	119(5.1%)	403(17.3%)	403(17.4%)	403(17.5%)
09-19	154(6.6%)	253(10.9%)	253(10.1%)	253(10.11%)
09-22	158(6.8%)	262(11.2%)	248(10.6%)	260(11.2%)
09-27	169(7.3%)	278(11.9%)	219(9.4%)	304(13.0%)
10-03	163(7.0%)	292(12.5%)	307(13.2%)	216(9.3%)
10-10	182(7.8%)	297(12.7%)	272(11.7%)	319(13.7%)
10-18	215(9.2%)	208(8.9%)	256(11.0%)	268(11.5%)
10-28	207(8.9%)	418(17.9%)	306(13.1%)	327(14.0%)
完全甲基化位点数(比例) Fully methylation loci(rate)
09-17	1 215(52.1%)	959(41.2%)	959(41.3%)	959(41.4%)
09-19	1 235(53.0%)	1 136(48.8%)	1 136(48.9%)	1 136(48.1%)
09-22	1 404(60.3%)	1 323(56.8%)	1 334(57.3%)	1 304(56.0%)
09-27	1 306(56.1%)	1 341(57.6%)	1 441(61.8%)	1 280(54.9%)
10-03	1 419(60.9%)	1 387(59.5%)	1 293(55.5%)	1 472(63.2%)
10-10	1 367(58.7%)	1 321(56.7%)	1 378(59.1%)	1 337(57.4%)
10-18	1 271(54.5%)	1 351(58.0%)	1 298(55.7%)	1 092(46.9%)
10-28	1 262(54.2%)	1 010(43.3%)	1 186(50.9%)	1 267(54.4%)
总甲基化位点数(比例) Total methylation loci(rate)
09-17	1 334(57.3%)	1 362(58.5%)	1 362(58.6%)	1 362(58.7%)
09-19	1 389(59.6%)	1 389(59.6%)	1 389(59.7%)	1 389(59.8%)
09-22	1 562(67.0%)	1 585(68.0%)	1 582(67.9%)	1 564(67.1%)
09-27	1 475(63.3%)	1 619(69.5%)	1 660(71.2%)	1 584(68.0%)
10-03	1 582(67.9%)	1 679(72.1%)	1 600(68.7%)	1 688(72.4%)
10-10	1 549(66.5%)	1 618(69.4%)	1 650(70.8%)	1 656(71.1%)
10-18	1 486(63.8%)	1 559(66.9%)	1 554(66.7%)	1 360(58.4%)
10 -28	1 469(63.0%)	1 428(61.3%)	1 492(64.0%)	1 594(68.4%)

甲基化模式Methylation patterns			位点数(比例) Loci(rate)
带型 Banding patterns	对照 Control (1 d)	对照 Control	3 d	6 d	11 d	17 d	24 d	32 d	42 d
A	不变Same methylation level		1 640 (70.4%)	1 445 (62.0%)	1 569 (67.3%)	1 440 (61.8%)	1 491 (64.0%)	1 488 (63.9%)	1 456 (62.5%)
A1	1, 1	1, 1	751	614	688	597	631	664	651
A2	1, 0	1, 0	27	30	31	26	29	32	35
A3	0, 1	0, 1	26	49	42	58	53	37	45
A4	0, 0	0, 0	836	752	808	759	778	755	725
B	去甲基化 Demethylation		275 (11.8%)	342 (14.7%)	317 (13.6%)	348 (14.9%)	335 (14.4%)	373 (16.0%)	410 (17.6%)
B1	1, 0	1, 1	31	21	33	27	29	34	35
B2	0, 1	1, 1	53	46	65	53	57	67	73
B3	0, 0	1, 1	106	87	69	71	64	79	102
B4	0, 0	1, 0	51	70	70	74	87	91	94
B5	0, 0	0, 1	34	118	80	123	98	102	106
C	超甲基化 Hypermethylation		383 (16.4%)	509 (21.8%)	411 (17.6%)	508 (21.8%)	473 (20.3%)	439 (18.8%)	435 (18.7%)
C1	1, 1	1, 0	53	45	53	52	52	74	62
C2	1, 1	0, 1	57	142	82	143	128	90	94
C3	1, 1	0, 0	135	195	173	204	185	168	189
C4	0, 1	0, 0	86	80	66	66	64	66	54
C5	1, 0	0, 0	52	47	37	43	44	41	36
D	不定型Methylation pattern changed		32 (1.4%)	34 (1.5%)	33 (1.4%)	34 (1.5%)	31 (1.3%)	30 (1.3%)	29 (1.2%)
D1	0, 1	1, 0	23	13	15	11	14	18	16
D2	1, 0	0, 1	9	21	18	23	17	12	13
合计Total			2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)

Variation in Physiological and Biochemical Properties and DNA Methylation Patterns during Autumn Flowering of Tree Peony(Paeonia Suffruticosa)

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 16

References 0

Related Articles 8

Recommended Articles

Metrics

Comments

甲基化模式Methylation patterns			位点数(比例) Loci(rate)
带型 Banding patterns	对照 Control (1 d)	处理A Treatment A	1 d	3 d	6 d	11 d	17 d	24 d	32 d	42 d
A	不变Same methylation level		1 386 (59.5%)	1 476 (63.3%)	1 306 (56.1%)	1 251 (53.7%)	1 209 (51.9%)	1 253 (53.8%)	1 304 (56.0%)	1 179 (50.6%)
A1	1, 1	1, 1	710	712	582	537	507	548	572	600
A2	1, 0	1, 0	35	38	29	30	31	38	29	37
A3	0, 1	0, 1	21	37	37	57	44	60	39	29
A4	0, 0	0, 0	620	689	658	627	627	607	664	513
B	去甲基化 Demethylation		527 (22.6%)	454 (19.5%)	440 (18.9%)	471 (20.2%)	468 (20.1%)	489 (21.0%)	451 (19.4%)	626 (26.9%)
B1	1, 0	1, 1	42	40	25	28	24	21	30	38
B2	0, 1	1, 1	78	76	46	43	44	48	58	74
B3	0, 0	1, 1	138	113	92	103	76	95	111	190
B4	0, 0	1, 0	212	119	147	131	158	145	95	211
B5	0, 0	0, 1	57	106	130	166	166	180	157	113
C	超甲基化 Hypermethylation		368 (15.8%)	366 (15.7%)	537 (23.0%)	553 (23.7%)	608 (26.1%)	541 (23.2%)	538 (23.1%)	468 (20.1%)
C1	1, 1	1, 0	116	76	65	89	81	93	70	127
C2	1, 1	0, 1	66	92	148	199	162	185	120	110
C3	1, 1	0, 0	104	116	201	171	246	170	234	159
C4	1, 0	0, 0	33	27	39	34	41	34	37	30
C5	0, 1	0, 0	49	55	84	60	78	59	77	42
D	不定型Methylation pattern changed		49 (2.1%)	34 (1.5%)	47 (2.0%)	55 (2.4%)	45 (1.9%)	47 (2.0%)	37 (1.6%)	57 (2.4%)
D1	0, 1	1, 0	40	20	21	28	22	21	14	43
D2	1, 0	0, 1	9	14	26	27	23	26	23	14
合计Total			2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)

甲基化模式Methylation patterns			位点数(比例)Loci(rate)
带型 Banding patterns	对照 Control (1 d)	处理B Treatment B	1 d	3 d	6 d	11 d	17 d	24 d	32 d	42 d
A	不变Same methylation level		1 386 (59.5%)	1 476 (63.3%)	1 254 (53.8%)	1 256 (53.9%)	1 260 (54.1%)	1 266 (54.3%)	1 284 (55.1%)	1 313 (56.4%)
A1	1, 1	1, 1	710	712	557	525	549	528	578	614
A2	1, 0	1, 0	35	38	26	29	31	28	34	34
A3	0, 1	0, 1	21	37	43	49	35	42	37	42
A4	0, 0	0, 0	620	689	628	653	645	668	635	623
B	去甲基化 Demethylation		527 (22.6%)	454 (19.5%)	485 (20.8%)	439 (18.8%)	467 (20.0%)	433 (18.6%)	485 (20.8%)	501 (21.5%)
B1	1, 0	1, 1	42	40	32	23	30	26	32	33
B2	0, 1	1, 1	78	76	54	42	55	48	61	64
B3	0, 0	1, 1	138	113	105	80	96	78	105	127
B4	0, 0	1, 0	212	119	139	122	156	136	125	152
B5	0, 0	0, 1	57	106	155	172	130	145	162	125
C	超甲基化 Hypermethylation		368 (15.8%)	366 (15.7%)	548 (23.5%)	591 (25.4%)	557 (23.9%)	595 (25.5%)	523 (22.4%)	474 (20.3%)
C1	1, 1	1, 0	116	76	60	51	90	91	77	98
C2	1, 1	0, 1	66	92	173	192	126	139	131	152
C3	1, 1	0, 0	104	116	206	228	231	238	210	132
C4	1, 0	0, 0	33	27	41	40	42	46	35	32
C5	0, 1	0, 0	49	55	68	80	68	81	70	60
D	不定型Methylation pattern changed		49 (2.1%)	34 (1.5%)	43 (1.8%)	44 (1.9%)	46 (2.0%)	36 (1.5%)	38 (1.6%)	42 (1.8%)
D1	0, 1	1, 0	40	20	23	17	30	17	20	22
D2	1, 0	0, 1	9	14	20	27	16	19	18	20
合计Total			2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)	2 330 (100.0%)

[1]	Li Yuanyuan, Zhang Kai, Li Shuangwen, Yan Shanchun. Effects of Defoliations on the Chlorophyll Contents and Biomass of the Poplar (Populus simonii×P. nigra) and Larix gmelinii Seedlings [J]. Scientia Silvae Sinicae, 2015, 51(3): 93-101.
[2]	Cao Xibing, Zhao Zhenli, Fan Guoqiang, Deng Minjie, Dong Yanpeng. Effect of Methyl Methanesulphonate on DNA Methylation of Witches’Broom Seedlings of Paulownia tomentosa [J]. Scientia Silvae Sinicae, 2014, 50(3): 99-108.
[3]	Zhao Hua;Zhou Tiancang;Cheng Jingjing;Li Xiaohu;Huang Lili. Control Effect of Triazole Fungicides in controlling Marssonina coronaria in Vitro and in Field [J]. Scientia Silvae Sinicae, 2009, 12(3): 68-73.
[4]	Li Ming;Zhai Xiaoqiao;Fan Guoqiang;Zhang Bianli;Liu Fe. Effect of Oxytetracycline on the Morphology of Seedling with Witches' Broom and DNA Methylation Level of Paulownia tomentosa×P. fortunei [J]. Scientia Silvae Sinicae, 2008, 44(9): 152-156.
[5]	Jiang Zhongzhu;Chen Xiangwei. Effect of Paclobutrazol on Drought-Resistance of Populus alba × Populus berolinensis, Ulmus pumila, and Betula platyphylla [J]. Scientia Silvae Sinicae, 2006, 42(8): 130-134.
[6]	Zhou Zhang;yi Li Jinghui. EFFECT OF DEFOLIATION ON RESISTANCE OF CHINESE PINE TO DENDROLIMUS SPECTABILIS AND THE PEST CONTROL STRATEGY [J]. , 1993, 29(3): 220-226.
[7]	Tong Benqun;Hao Zhongying. THE PATTERN OF ENDOGENOUS HORMONES OF PISTILLATE FLOWER DIFFERENTIATION IN WALNUT (JUGLANS REGIA L.) [J]. , 1991, 27(4): 401-409.
[8]	Shen Xihuan;Wang Qing;P. C. Oden. FLOWER-INDUCTIVE TREATMENT AND QUANTITATIVE ANALYSIS OF GIBBERELLINS IN GRAFTING PLANTS OF NORWAY SPRUCE (PICEA ABIES) [J]. , 1991, 27(2): 168-172.