油茶花发育转录组测序及相关基因表达分析

doi:10.11707/j.1001-7488.20140905

Abstract

Abstract:

In this study, total RNA of stem apex and bud in Camellia oleifera was sequenced and its flowering relation genes were analysis. The results showed that transcriptome sequencing revealed a total of 28 448 847 reads, 5 742 023 480 bp data, and 46.52% GC content in whole genome. The Unigenes numbers with more than 200 bp were 94 476, and the Unigenes were very similar with grape's. The N50 length was 806 bp, and 1 kbp or longer Unigenes were 12 643, accounting for 13.38% of total Unigenes database. The annotation number of Unigenes function in each database was:9 095 Unigenes in COG, 27 201 Unigenes in GO, 6 431 Unigenes in KEGG, 24 534 Unigenes in Swissprot, 36 393 Unigenes in TrEMBL, 36 400 Unigenes in Nr, and 30 858 Unigenes in Nt. The expression profile showed that FLC, FCA, FT expression were lower than AP1, AP2, PI expression in the stem apex. The FT gene (ID:Unigene60063) may be a key gene for Camellia flower, and PI genes (ID:Unigene56059) are closely related with the stamen development.

Key words: Camellia oleifera, transcriptome, high-throughout sequencing, flowering genes, flowering way

CLC Number:

S718.46

Hu Yulin, Yao Xiaohua, Ren Huadong, Wang Kailiang, Lin Ping. Sequencing of Transcriptome Relevant to Flowering and Analysis of Floral-Related Genes Expression in Camellia oleifera[J]. Scientia Silvae Sinicae, 2014, 50(9): 36-43.

References

陈英, 江香梅, 张露,等. 2011. 基于油茶组59万条EST序列的转录组学初步分析. 林业科学, 47(2): 161-163.
(Chen Y, Jiang X M, Zhang L, et al. 2011. Transcriptome characterization for camellia sect. Oleifera based on the 592 499 ESTs. Scientia Silvae Sinicae, 47(2): 161-163. [in Chinese] )
高本年. 1981. 普通油茶适宜花期的初步探讨. 江苏林业科技, (4): 41-44.
韩宁林, 赵学民. 2009. 油茶高产品种栽培. 北京: 中国农业出版社.
黎章矩, 施拱生, 吴德晔. 1992. 油茶生育规律及其影响因子研究. 经济林研究, 10(1): 21-26.
(Li Z J, Shi G S, Wu D Y. 1992. Influencing factors on oil content of Oiltea camellia. Economic Forest Researches, 10(1): 21-26. [in Chinese] )
林萍, 曹永庆, 姚小华, 等. 2011. 普通油茶种子4个发育时期的转录组分析. 分子植物育种,9(4): 498-505.
(Lin P, Cao Y Q, Yao X H, et al. 2011. Transcriptome analysis of Camellia oleifera abel seed in four development stages. Molecular Plant Breeding,9(4): 498-505. [in Chinese] )
林少韩, 徐乃焕. 1981. 油茶花期生态及结实力研究. 林业科学, 17(2): 113-122.
(Lin S H, Xu N H. 1981. A study on the ecological factor of blossoming period and fruiting ability of Camellia oleifera. Scientia Silvae Sinicae, 17(2): 113-122. [in Chinese] )
潘瑞炽. 2004. 植物生理学. 北京: 高等教育出版社.
(Pan R C. 2004. Plant Physiology. Beijing: Higher Education Press. [in Chinese] )
唐光旭. 1984. 油茶春梢生长发育与物质转化的研究. 经济林研究, 2(2): 44-51.
(Tang G X. 1984. Studies on the growth of the spring shoots of the tea oil trees and its substance transformation. Economic Forest Researches, 2(2): 44-51. [in Chinese] )
王文杰, 陈长庚, 程剑. 2007. 茶油在医药保健方面的积极作用. 中国食物与营养, 9(4): 48-51.
(Wang W J, Chen C G, Cheng J. 2007. Positive effects of tea-oil in medicine and health-care. Food and Nutrition in China, 9(4): 48-51. [in Chinese] )
王湘南, 蒋丽娟, 马力, 等. 2011. 油茶花芽分化的形态解剖学特征观测. 中南林业科技大学学报, 31(8): 22-27.
(Wang X N, Jiang L J, Ma L, et al. 2011. Observation on morphological and anatomical characteristics of the flower bud differentiation on Camellia oleifera. Journal of Central South University of Forestry & Technology, 31(8): 22-27. [in Chinese] )
王忠. 2002. 植物生理学. 北京: 中国农业出版社.
(Wang Z. 2002. Plant Physiology. Beijing: China Agriculture Press. [in Chinese] )
袁德义, 邹锋, 范晓明, 等. 2011. 油茶花芽分化及雌雄配子体发育的研究. 中南林业科技大学学报, 31(3): 65-70.
(Yuan D Y, Zou F, Fan X M, et al. 2011. Flower bud differentiation and development of male and female gametophytes in Camellia oleifera. Journal of Central South University of Forestry & Technology, 31(3): 65-70. [in Chinese] )
曾燕如, 黎章矩, 戴文圣. 2009. 油茶开花习性的观察研究. 浙江林学院学报, 26(6): 802 - 809.
(Zeng Y R, Li Z J, Dai W S. 2009. Flowering habits in Camellia oleifera. Journal of Zhejiang Forestry College, 26(6): 802 - 809.
查小华, 江国强, 雷小林, 等. 2012. 油茶结果枝与成花相关性研究. 江西林业科技,(2): 12-14.
(Zha X H, Jiang G Q, Lei X L, et al. 2012. Correlation between bearing branch and flower formation in Camellia oleifera. Jiangxi Forestry Science and Technology,(2): 12-14. [in Chinese] )
庄瑞林. 2008. 中国油茶. 北京: 中国林业出版社.
Blazquez M A. 2001. Flower development pathways. Cell Science at a Glance, 113:3547-3548.
Bowman J L, Alvarez J, Weigel D, et al. 1993. Control of flower development in Arabidopsis thaliana by APETALA 1 and interacting genes. Development, 119: 721-743.
Cerdan P D, Chory J. 2003. Regulation of flowering time by light quality. Nature, 423: 881-885.
Hoen P A C, Ariyurek Y, Thygesen H H, et al. 2008. Deep sequencing-based expression analysis shows major advances in robustness, resolution and inter-lab portability over five microarray platforms. Nucleic Acids Research, 36(21): e141.
Howell S H. 1998. Molecular genetic of plant development. London: Cambridge University Press.
Huang T, Bhlenius H, Eriksson S, et al. 2005. The mRNA of the Arabidopsis gene FT moves from leaf to shoot apex and induces flowering. Science, 309(5741): 1694-1696.
Jack T. 2004. Molecular and genetic mechanisms of floral control. Plant Cell, 16(suppl 1): S1-S17.
Lander E S, Linton L M, Birren B, et al. 2001. Initial sequencing and analysis of the human genome. Nature, 409(6822): 860-921.
Mandel M A, Gustafson-Brown C, Savidge B, et al. 1992. Molecular characterization of the Arabidopsis floral homeotic gene APETALA 1. Nature, 360: 273-277.
Mortazavi A, Williams B A, Wold B, et al. 2008. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nature Methods, 5(7): 621-628.
Nagalakshmi U,Wang Z,Waern K, et al. 2008. The transcriptional landscape of the yeast genome defined by RNA sequencing. Science, 320( 5881) : 1344-1349.
Rosenkranz R, Borodina T, Lehrach H, et al. 2008. Characterizing the mouse ES cell transcriptome with Illumina sequencing. Genomics, 92(4) : 187-194.
Velculescu V E, Zhang L, Zhou W, et al. 1997. Characterization of the yeast transcriptome. Cell, 88(2): 243-251.
Weigel D, Alvarez J, Smyth D R, et al. 1992. Leafy controls floral meristem identity in Arabidopsis. Cell, 69(5): 843-859.
Wilhelm B T, Landry J R. 2009. RNA-Seq-quantitative measurement of expression through massively parallel RNA-sequencing. Methods, 48(3): 249-257.
Zeevaart J A D. 2008. Leaf-produced floral signals. Curr Opin in Plant Biol, 11(5):541-547.
Zhou C F, Lin P, Yao X H, et al. 2013. Selection of reference genes for quantitative real-time PCR in six oil-tea camellia based on RNA-seq. Molecular Biology, 47(6): 836-851.

[1]	Han Xiaohong, Lu Ciding, Hua Yin, Lin Haoyu, Shi Yufei, Wu Songqing, Zhang Feiping, Liang Guanghong. Phylogenetic Analysis of Transcriptome and Three Detoxification Enzyme Families Related Genes in Anoplophora chinensis (Coleoptera: Cerambycidae) [J]. Scientia Silvae Sinicae, 2019, 55(5): 104-113.
[2]	Zhang Enliang, Ma Lingling, Yang Rutong, Li Linfang, Wang Qing, Li Ya, Wang Peng. Transcriptome Profiling of IBA-Induced Adventitious Root Formation in Softwood Cuttings of Catalpa bungei ‘Yu-1’ [J]. Scientia Silvae Sinicae, 2018, 54(5): 48-61.
[3]	Mao Weibing, Chen Faju, Wang Changlan, Liang Hongwei. Transcriptome Sequencing and Analysis of Male Sterile Flower Buds in Catalpa bungei [J]. Scientia Silvae Sinicae, 2017, 53(6): 141-150.
[4]	Shi Xiaodong, Zhu Xuehui, Sheng Yuzhen, Zhuang Guoqing, Chen Fang. Development of SSR Markers Based on Transcriptome Sequence of Phoebe zhennan [J]. Scientia Silvae Sinicae, 2016, 52(11): 71-78.
[5]	Zhang Zhen, Zhang Hanguo, Mo Chi, Zhang Lei. Transcriptome Sequencing Analysis and Development of EST-SSR Markers for Pinus koraiensis [J]. Scientia Silvae Sinicae, 2015, 51(8): 114-120.
[6]	Chen Hao, Tan Xiaofeng. Identification of α-Linolenic Acid Metabolism Pathway Based on Transcriptome Data of Vernicia fordii Kernels during Tung Oil Synthesis Stage [J]. Scientia Silvae Sinicae, 2015, 51(3): 41-48.
[7]	Wen Yafeng, Han Wenjun, Zhou Hong, Xu Gangbiao. SSR Mining and Development of EST-SSR Markers for Cunninghamia lanceolata Based on Transcriptome Sequences [J]. Scientia Silvae Sinicae, 2015, 51(11): 40-49.
[8]	Jiang Nan, Tan Xiaofeng, Zhang Lin, Zeng Yanling. Gene Analysis of α-Linolenic Acid Metabolism of Camellia oleifera Seeds Based on RNA-Seq [J]. Scientia Silvae Sinicae, 2014, 50(8): 68-75.
[9]	Sun Ying, Tan Xiaofeng, Luo Min, Li Jian. The Sequencing Analysis of Transcriptome of Vernicia fordii Flower Buds at Two Development Stages [J]. Scientia Silvae Sinicae, 2014, 50(5): 70-74.
[10]	Niu Shihui, Yuan Huwei, Chen Xiaoyang, Li Wei. Microarray Analysis of Large Scale Gene Expression Profiles between Male and Female Cones of Pinus tabulaeformis [J]. Scientia Silvae Sinicae, 2013, 49(9): 46-51.
[11]	Deng Minjie, Dong Yanpeng, Zhao Zhenli, Zhang Xiaoshen, Fan Guoqiang. Illumina-Based de novo Sequencing and Characterization of the Transcriptome of Paulownia Plant [J]. Scientia Silvae Sinicae, 2013, 49(6): 30-36.
[12]	Huang Haiyan;Du Hongyan;Wuyun Tana;Liu Panfeng. Development of SSR Molecular Markers Based on Transcriptome Sequencing of Eucommia ulmoides [J]. , 2013, 49(5): 176-181.
[13]	Wei Zhigang;Qian Tingting;Zhang Kaixuan;Liu Guanjun;Liu Guifeng;Yang Chuanping. Effect of Exogenous GA₃ on Flowering Genes Expression of Betula platyphylla by Real-Time Fluorescent Quantitative PCR [J]. Scientia Silvae Sinicae, 2011, 47(7): 187-192.
[14]	Chen Ying;Jiang Xiangmei;Zhang Lu;Zhang Xinye;Xiang Dongyun;Wen Qiang;Jiang Cong;Wang Guangping;Huang Minren;Xu Li'an. Transcriptome Characterization for Camellia Sect. Oleifera Based on the 592 499 ESTs [J]. Scientia Silvae Sinicae, 2011, 47(2): 161-163.

Sequencing of Transcriptome Relevant to Flowering and Analysis of Floral-Related Genes Expression in Camellia oleifera

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments