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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (6): 30-36.doi: 10.11707/j.1001-7488.20130605

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Illumina-Based de novo Sequencing and Characterization of the Transcriptome of Paulownia Plant

Deng Minjie1,2, Dong Yanpeng1,2, Zhao Zhenli1,2, Zhang Xiaoshen1,3, Fan Guoqiang1,2   

  1. 1. Institute of Paulownia, Henan Agricultural University Zhengzhou 450002;
    2. College of Forestry, Henan Agricultural University Zhengzhou 450002;
    3. Zhengzhou Research Institute of Agriculture and Forestry Sciences Zhengzhou 451400
  • Received:2013-01-29 Revised:2013-04-20 Online:2013-06-25 Published:2013-07-16

Abstract:

The transcriptome Paulownia plant was sequenced by using Illumina technology, and the functional annotations and metabolic pathways analyses of some unigenes were conducted in this present paper. The results indicated that 188 019 unigenes with an average length of 1 410 bp and N50 value of 2 278 bp were obtained with de novo assembly. Comparison with NCBI and Swiss-Prot protein databases showed that 120 808 and 85 880 unigenes had homology with the genes from other species, respectively. The 41 914 unigenes were able to be assigned to 25 categories with the COG (Clusters of Orthologous Groups) database. Functional annotation against KEGG (Kyoto Encyclopedia of Genes and Genomes) database identified 43 553 unigenes which were mapped to 215 metabolic pathways. Moreover, some Paulownia unigenes related to lignin biosynthesis were found using this information platform. The result provides reference for future excavations of new genes, as well as screening and identifying miRNA of Paulownia plants.

Key words: Paulownia, transcriptome, Illumina sequencing, de novo assembly

CLC Number: