基于油茶组59万条EST序列的转录组学初步分析

doi:10.11707/j.1001-7488.20110222

Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (2): 161-163.doi: 10.11707/j.1001-7488.20110222

Transcriptome Characterization for Camellia Sect. Oleifera Based on the 592 499 ESTs

Chen Ying¹, Jiang Xiangmei², Zhang Lu³, Zhang Xinye⁴, Xiang Dongyun⁵, Wen Qiang^1,2, Jiang Cong¹, Wang Guangping¹, Huang Minren¹, Xu Li'an¹

1. Nanjing Forestry University Nanjing 210037;2. Jiangxi Academy of Forestry Nanchang 330032;3. Jiangxi Agricultural University Nanchang 330045;4. Hubei Academy of Forestry Wuhan 430079;5. Guangxi Academy of Forestry Nanning 530002

Received:2010-10-22 Revised:2010-11-24 Online:2011-02-25 Published:2011-02-25

Abstract

Abstract:

To investigate gene diversity and expression pattern in Camellia Sect. Oleifera, 454 GS FLX platform and Titanium regent were used to produce expressed sequence tags (ESTs) from flower buds of 3 Camellia species. A total of 5 920 499 ESTs were generated. Clustering those sequences twice by Cap3 produced 15 733 contigs and 20 606 singlets in C. oleifera , 19 397 contigs and 26 882 singlets in C. chekiangoleosa , 14 779 contigs and 25 389 singlets in C. brevistyla . The GC gradients in 3 transcriptomes basically followed a normal distribution. In additional all contigs and singlets were functionally categorized into GO(Gene Ontology) categories respectively.

Key words: Camellia, transcriptome, 454 GS FLX, expressed sequence tags

CLC Number:

Chen Ying;Jiang Xiangmei;Zhang Lu;Zhang Xinye;Xiang Dongyun;Wen Qiang;Jiang Cong;Wang Guangping;Huang Minren;Xu Li'an. Transcriptome Characterization for Camellia Sect. Oleifera Based on the 592 499 ESTs[J]. Scientia Silvae Sinicae, 2011, 47(2): 161-163.

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Transcriptome Characterization for Camellia Sect. Oleifera Based on the 592 499 ESTs

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